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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM321 · Revised 4/15
www.promega.com
5.B. Setting Up Samples and Collecting Input Data (continued)
3.
For SEV methods, add the sample to the associated cartridge, and place the cartridge in the instrument position
corresponding to the
Position
number on the screen. Select the
Eluate ID
option, and select
Manual
. Use the
keyboard to type in the data required. The data will appear on the screen next to
Eluate ID
. Place the tube in the
appropriate Elution Tube slot at the front of the platform next to the cartridge. Review the data. All data should
match. If they do not match, you can reselect the option to change the information.
4.
For LEV methods, select the
Cart. ID
option and the cartridge to which the sample will be added. Select
Manual
. Use the keyboard to type in the data required. The data will appear on the screen next to
Cart. ID
;
place the cartridge into the Cartridge Rack. Add the sample to the cartridge. Select the
Eluate ID
option, and
select
Manual
. Use the keyboard to type in the data required. The data will appear on the screen next to
Eluate ID
. Place the tube in the appropriate Elution Tube slot at the front of the LEV Cartridge Rack next to the
cartridge. Review the data. All data should match. If they do not match, you can reselect the option to change the
information.
5.
Select the
Kit Info
option, and select
Manual
. Add the kit information using the keyboard. The data will appear
on the screen next to
Kit Info
. Check that the kit expiration date has not passed.
6.
On-screen data can be removed from each option by highlighting the option and selecting the
Clear Selection
option. All data can be removed by selecting the
Clear All
option.
7. Select
Next Sample
to move to the next position. The appropriate
Position
number will be displayed, and the kit
box information will be carried over to the next position screen. You will not need to add the kit box data each
time. If more than one kit lot is used, the new kit lot number can be added once the cartridge and Elution Tube
from the new kit are added to the rack.
8.
Repeat Steps 2 to 5 for the next sample and associated cartridge and Elution Tube, and place in the required
position. Select
Next Sample
. Repeat until all samples are loaded.
9.
If there are less than 16 samples, select
Final Sample
when all samples are loaded. This will open the
Verify
Scanned Data
screen. This screen allows you to review the data. If any incorrect data are identified for a
sample, you can use the navigation buttons to return to the
Bar code Input
screen where Position 01 will be
shown. Use
Next Sample
to navigate to the required sample, and add the appropriate information.
10. For SEV runs, once all data have been verified, press the Run/Stop button to continue.
11. For LEV runs, once all data have been verified, place the LEV Cartridge Rack into the instrument, and press the
Run/Stop button to continue.
