Promega Maxwell 16 Forensic Technical Manual Download Page 1

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Maxwell

16 Forensic

Instrument Technical Manual

Printed in the USA

Revised

Instructions for Use

of Product

AS3060

Part# TM321

Summary of Contents for Maxwell 16 Forensic

Page 1: ...Maxwell 16 Forensic Instrument Technical Manual Printed in the USA Revised 4 15 Instructions for Use of Product AS3060 Part TM321...

Page 2: ...ic Instrument 13 3 A Unpacking 13 3 B Removing the Magnetic Rod Assembly Plunger Bar Platform Shipping Anchors 14 3 C UV Bulb Installation 14 3 D Maxwell Sample Track 15 3 E Operational Setup 16 4 Har...

Page 3: ...D Decontamination Using the UV Lamp 39 8 E Replacing the UV Bulb 40 8 F Periodic Maintenance 40 9 Troubleshooting 40 10 Warranties Service Agreements and Related Products 46 10 A Warranty Information...

Page 4: ...DNA from a wide variety of sample types The Maxwell 16 Forensic Instrument is a particle handler that uses paramagnetic particles as a purification medium to bind and wash nucleic acids It has two mod...

Page 5: ...mately 30 minutes Number of Samples up to 16 Weight 19 4kg 42 7lb Dimensions W D H 325 5 438 2 326 5mm 12 8 17 3 12 9 inches Power Requirements 100 240VAC 50 60Hz 2 1A Fuse 3A time lag fuse UV Bulb Av...

Page 6: ...mega representative regarding instrument disposal Consulter un repr sentant Promega local concernant l limination de l appareil Catalog number Num ro de catalogue 61 Serial number Num ro de s rie 1 D...

Page 7: ...orensic Instrument SEV components Components shown include Bar Code Reader Cable Bar Code Reader SEV and LEV Cartridge Racks SEV Elution Rack Power Cord UV Bulb SD Card Reader and cable SD Card RS 232...

Page 8: ...ers are securely inserted in their correct positions and orientation Failure to do so may result in damage to the instrument After each run verify that the Plungers were completely removed from the ma...

Page 9: ...isque lors du soul vement 6422MA Warning Biohazard Avertissement Risque biologique Warning UV light hazard Do not look directly at the UV light Avertissement Risque de lampe UV Ne regardez pas directe...

Page 10: ...an of the instrument install in a location that meets the following criteria Locate on a sturdy level surface Avoid dusty areas Choose a location that has good air circulation and is not exposed to di...

Page 11: ...ic Instrument The same button selections are available on the keypad and screen Both sets of buttons can be used to navigate within and between screens The Back and Run Stop buttons allow navigation b...

Page 12: ...Adaptor is included and can be used to connect the Maxwell 16 Forensic Instrument to a USB port on the computer The USB connector port is for a bar code reader and only allows bar code data to be impo...

Page 13: ...16 Platform Linear slides Linear slides Standard magnetic rods Plunger bar LEV magnetic rods Plunger bar Magnetic rod assembly C Maxwell 16 Platform Heated elution tube slots Reagent cartridge slots...

Page 14: ...is retained 3 Unpacking and Setting Up the Maxwell 16 Forensic Instrument 3 A Unpacking 1 Remove the accessory box power cord and literature package from the shipping container Slide the instrument ou...

Page 15: ...mediately turn off and unplug the instrument and remove the shipping anchors This will not result in permanent damage to the instrument 3 Open the instrument door and locate the Magnetic Rod Assembly...

Page 16: ...well Sample Track software you need to load the software onto the computer that will be connected to the Maxwell 16 Forensic Instrument See the Technical Manual supplied with the software for more inf...

Page 17: ...back of the instrument Figure 4 Connect the power cord to the back of the Maxwell 16 Forensic Instrument and plug the power cord into a wall outlet See Section 1 F for power requirements Switch the i...

Page 18: ...wish to change the language choose from the list in the Select Language screen You may need to scroll down to the option needed Once you have selected a language press the Run Stop button 8285TA Figu...

Page 19: ...ypad If they do not all change color press the back arrow button and repeat the calibration and confirmation The Touch Screen Configuration Successful message indicates that the calibration was succes...

Page 20: ...treatment for at least 1 hour The UV lamp also can be turned on from the Setup menu see Section 8 D 8208TA Figure 15 UV Settings screen Select the desired option on the UV screen then set the time for...

Page 21: ...be used to identify the instrument on reports 8210TA Figure 17 Unit Name screen Touching the screen to name an instrument will open a keyboard to add a unique identifier 7 User and PIN Setup This opti...

Page 22: ...tings screen selecting Exit will take you to the User Setup Complete screen where you can choose to add more users Selecting Yes will return you to the Select User screen where the added user is highl...

Page 23: ...sfer of run data from the instrument to a computer if you are using the Maxwell Sample Track software see Section 6 or it allows deletion of run data from the instrument Selecting Setup opens the Maxw...

Page 24: ...dware mode are appropriate for the purification kit being used If the installed hardware is not correct for the kit being used remove the installed Magnetic Rod Assembly and replace it with the approp...

Page 25: ...d users a list of user names will be shown Select the appropriate user and enter the matching PIN if appropriate Press the Run Stop button 2 Press the Run Stop button A Verification screen indicates w...

Page 26: ...ed 3 the sample position within the Maxwell 16 Forensic Instrument where the cartridge was placed and 4 the Elution Tube used to collect the eluate Enter the sample and kit information into the Maxwel...

Page 27: ...next to the Eluate ID option Review the scanned data All three codes should match If they do not match you can reselect the option to change and rescan the information 4 For LEV methods scan the cart...

Page 28: ...5TA Figure 24 Verify Scanned Data screen 10 If any incorrect data are identified for a sample you can use the navigation buttons to return to the Bar code Input screen where Position 01 will be shown...

Page 29: ...change the information 5 Select the Kit Info option and select Manual Add the kit information using the keyboard The data will appear on the screen next to Kit Info Check that the kit expiration date...

Page 30: ...placed in the correct starting position If the instrument goes through a run without Plungers the magnetic rod assembly must be removed and cleaned see Section 8 B The cartridges containing samples m...

Page 31: ...more information If you are in the middle of a run you can choose to continue the purification procedure or abandon the run Continuing the run will not damage the Maxwell 16 Forensic Instrument but ma...

Page 32: ...To avoid particle transfer after SEV runs use a pipette tip to aspirate samples from the captured particles on the side of the blue elution tube 5 D Post Run UV Light Treatment If you have configured...

Page 33: ...ou will be notified that the file was sent to the printer Once printing is complete press the Run Stop button to close the File Sent screen 6 B Data File Transfer at a Later Time Files may be exported...

Page 34: ...the instructions in Section 8 before removing the existing hardware Wear gloves when changing the hardware to avoid exposure to any potential contaminants Changing from SEV to LEV Hardware 1 Select Ha...

Page 35: ...Plunger Bar Adaptor onto the bottom of the remaining arm Make sure that the side containing the screws faces upward Several magnets will click and hold the LEV Plunger Bar Adaptor in place once it is...

Page 36: ...physical hardware installed turn the instrument off install the appropriate hardware and restart the machine The hardware indicated on screen should match the physical hardware installed Important Whe...

Page 37: ...and remove it Pull down on the LEV Plunger Bar Adaptor to release the magnets that hold it in place Note Pull down at an angle so that the back of the magnet is released first 6 Remove the black LEV...

Page 38: ...nstalled Running the instrument when the hardware mode shown does not match the physical hardware installed will damage the instrument 7 B Changing the Instrument Firmware Configuration Resetting the...

Page 39: ...he User Name Setup screen select a name and press the Run Stop button Select Add Edit User a keyboard will be displayed and a new user name can be added Follow the on screen instructions to add the ne...

Page 40: ...uire multiple wipes 3 If the Magnetic Rod Assembly cannot be cleaned contact Promega for assistance 4 Replace the Magnetic Rod Assembly and firmly tighten the three thumbscrews 5 Ensure that the hardw...

Page 41: ...nto UV lamp socket and twist a quarter turn until it is firmly in place The UV bulb contains mercury and must be disposed of properly To dispose of a bulb please follow your institutional requirements...

Page 42: ...mega representative for service Wrong language is displayed If the wrong language is displayed the Language Setup screen can be accessed by switching the instrument off and on In the Home screen press...

Page 43: ...accumulated lamp time the following error message appears 8225TA Figure 40 UV Lamp Time This message means that the UV Bulb has reached the limit of its rated life span and should be replaced See Sec...

Page 44: ...resentative for service A heater problem may affect yield Installed hardware does not match the displayed Turn the machine off and install the appropriate hardware hardware mode When the machine is po...

Page 45: ...ative for service Calibration error Error Code 2 Error code 2 indicates a Plunger Bar calibration error The Plunger Bar is shown in Figure 6 Verify that there are no solid particulates inside well 1 o...

Page 46: ...a point where the magnetic particles were captured on the outside of the Plungers manually move the Plungers up and down in the wells to wash the particles off Switch the instrument on Note the UV li...

Page 47: ...n to the standard limited warranty that comes with the Maxwell 16 Forensic Instrument a premier warranty and various service agreement options are available for purchase If you purchased a premier war...

Page 48: ...u can continue to receive the same comprehensive service and support from Promega as you did when your system was under warranty The Standard Service Agreement covers all parts labor and shipping to a...

Page 49: ...ified by turning the machine off then on again The initial screen will display the version number of the firmware loaded on the instrument Please note the firmware version currently installed on your...

Page 50: ...igure 42 Utilities Menu screen In the Utilities Menu screen select Service Report to view and print a current service report The report will list the report date instrument serial number date when las...

Page 51: ...ittle maintenance If a problem arises with your instrument contact your local Promega representative for support Visit the Promega Web site at www promega com for contact information for the Promega b...

Page 52: ...pping Anchor thumbscrews Figure 9 and tighten by hand to anchor the platform in place for shipment By hand gently lower the Plunger Bar and Magnetic Rod Assembly Replace the Magnetic Rod Plunger Bar s...

Page 53: ...updated to correct the labeling of the Back and Front of the Magnetic Rod Assembly a Patent Pending Maxwell is a registered trademark of Promega Corporation DNA IQ is a trademark of Promega Corporati...

Page 54: ...from the instrument surfaces Repeat the procedure as many times as required to effectively disinfect and decontaminate the instrument Failure to confirm disinfection and decontamination will result i...

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