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3 First Steps  |  

3.7 Adjusting the Resolution and Depth of Field

24

Primotech  |  Document 430055-9044-008 V.1.0; 03 / 2014

Procedure

1

To adjust the aperture diaphragm for transmitted light, slide the lever left 

1

(open) and right 

2

 (close).

1

2

2

To adjust the aperture diaphragm for reflected light, turn the wheel up 

1

(open) and down 

2

 (close).

1

2

3

To achieve an optimal image, the size of the cone should be matched to the
aperture of the objective.

INFO

¢

Altering the diaphragm also affects the brightness of the sample but it
should not be used for this purpose.

To change the brightness, adjust the illumination or insert a filter. For more
information, see Adjusting the Illumination Properties [

}

 21].

¢

If you select a different objective you need to subsequently re-adjust the
corresponding aperture diaphragm.

Summary of Contents for D/A MAT

Page 1: ...Instruction Manual Primotech Upright Microscope...

Page 2: ...olations will be prosecuted The use of general descriptive names registered names trademarks etc in this document does not imply even in the absence of a specific statement that such names are exempt...

Page 3: ...ling the Microscope 15 3 3 Inserting the Sample 17 3 4 Adjusting the Eyepieces 18 3 5 Selecting Objectives and Focusing 20 3 6 Adjusting the Illumination Properties 21 3 6 1 Adjusting the Illumination...

Page 4: ...4 Replacing the Light Source 43 6 5 Changing the Microscope Network Adapter 44 7 Maintenance and Disposal 45 7 1 Routine Cleaning and Care 45 7 2 Corrective and Preventive Maintenance 46 7 3 Support...

Page 5: ...Its design and intuitive controls result in a simple robust easy to use yet powerful microscope that helps you inspect a wide range of samples Primotech microscopes can also be connected to an iPad t...

Page 6: ...tech MAT Primotech D A MAT Primotech D A POL Primotech D POL Conoscopy Reflected light Yes Yes Yes Transmitted light Yes Yes Yes Yes Condenser K hler K hler K hler Stage X Y X Y ESD Rotatable Rotatabl...

Page 7: ...he majority of the documentation applies to all Primotech microscopes General exceptions Chapter 4 only applies to Primotech D A MAT Primotech D A POL Primotech D POL Conoscopy Chapter 5 only applies...

Page 8: ...areas in the presence of volatile anesthetics or in the presence of combustible solvents such as alcohol benzene or similar chemicals INFO The Safety Information document is also considered to be part...

Page 9: ...mple The light passes through the sample before being focused into the eyepieces Transmitted light is particularly suitable for the following scenarios Thin samples Polarization examinations Conoscopy...

Page 10: ...following main components For a detailed list of the components of your microscope see Scope of Delivery 56 9 1 2 4 6 5 3 7 8 1 Eyepieces 2 Intermediate tube 3 Objectives 4 Stage 5 Condenser and aper...

Page 11: ...e stand contains the following components and controls 1 DIN 6x20 slot for compensators 2 Objective centering screws 3 Reflected light intensity indicators 4 Condenser limit screw 5 Reflected light in...

Page 12: ...imotech MAT Primotech D A MAT Primotech D A POL 1 Tube mounting screw 2 Slot for analyzer 3 Slot for polarizer reflected light 4 Slot for filters e g color 5 Aperture diaphragm reflected light 6 Obliq...

Page 13: ...X Y stage is supplied with the following microscopes Primotech MAT Primotech D A MAT 1 Object guide 2 Move sample along y axis 3 Move sample along x axis 1 3 2 The rotatable stage is supplied with the...

Page 14: ...condenser is supplied with the following microscopes Primotech D A MAT Primotech D A POL Primotech D POL Conoscopy 1 Adjust condenser position 2 Rotatable polarizer transmitted light Not available fo...

Page 15: ...Tube Stage rotatable or X Y stage as applicable Light sources transmitted or reflected as applicable Condenser if available Procedure 1 Place the microscope stand on a stable flat and smooth surface...

Page 16: ...e power socket of the tube and the rear of the microscope stand This does not apply to Primotech D POL Conoscopy 9 Plug the microscope into a power socket and press the power switch The lowest LED on...

Page 17: ...together with the stage Ensure that the stage is low enough so that none of the objectives collide with the sample when rotating the objective nosepiece 3 Place the sample on the center of the stage Y...

Page 18: ...en you see one round image when looking through the two eyepieces Viewing height Each separation has a high and a low viewing height Swing the eyepieces to their upper or lower position at the desired...

Page 19: ...at the sample through the left eyepiece with your left eye only 4 Raise or lower the stage until the sample is in focus For more information see Selecting Objectives and Focusing 20 5 Look at the sam...

Page 20: ...tly far below the objective that they do not collide 2 For Primotech D A POL and Primotech D POL Conoscopy ensure the objective is centered For more information see Centering the Objectives 33 Focusin...

Page 21: ...tted illumination Brightness of reflected illumination Color of reflected illumination Oblique illumination for reflected illumination INFO Reflected illumination is not available for Primotech D POL...

Page 22: ...a halogen light Procedure 1 To use a conversion filter with reflected light insert the filter slider into the vertical slot on the intermediate tube 2 To use a conversion filter with transmitted light...

Page 23: ...aphragm controls the size and shape of the cone of light from the condenser This in turn affects the resolution depth of field and contrast of the image Resolution refers to the size of object that ca...

Page 24: ...wheel up 1 open and down 2 close 1 2 3 To achieve an optimal image the size of the cone should be matched to the aperture of the objective INFO Altering the diaphragm also affects the brightness of t...

Page 25: ...r each objective This is performed using the calibration slide For more information see www zeiss com matscope installation The microscope network adapter MNA is used to connect the microscope to the...

Page 26: ...iPad to the wireless network Ensure the iPad is within range 3 Download and install the Matscope app from the Apple App Store For more information see www zeiss com matscope installation 4 Open the M...

Page 27: ...g microscopes have a condenser Primotech D A MAT Primotech D A POL Primotech D POL Conoscopy 4 2 Specifying the Condenser Position The condenser is located beneath the stage It concentrates the light...

Page 28: ...liding with the bottom of the sample by specifying the maximum height Z position of the condenser Procedure 1 Insert the 0 5 mm hex key in the condenser clamp screw 2 Loosen the condenser clamp screw...

Page 29: ...wing is only a guide the exact settings depend on your preferences Prerequisites The area of the sample in the field of view has high contrast The illumination brightness is low The upper limit of the...

Page 30: ...the luminous field diaphragm are in focus 6 Turn the condenser centering screws until the bright area is centered in the field of view 7 Open the luminous field diaphragm until the edge just disappear...

Page 31: ...ument 430055 9044 008 V 1 0 03 2014 31 9 Close the aperture diaphragm until the image has the optimal contrast This is typically the case when the bright area fills approximately 2 3 of the field of v...

Page 32: ...noscopy examinations as well as providing an introduction to the basic principles of such examinations Polarization and conoscopy can be performed with the following microscopes Primotech D A POL Prim...

Page 33: ...the center of rotation of the stage Therefore the objectives of Primotech D A POL and Primotech D POL Conoscopy can be centered The centering procedure should also always be performed after changing...

Page 34: ...2014 3 Insert the 0 5 mm hex key into the screws on either side of the objective 4 Turn the screws to move the object half the way back to the center of the crosshair 5 Repeat the steps above until th...

Page 35: ...th on either side of the sample The polarizing elements only allow light that oscillates in a certain direction to pass Polarizers and Analyzers The polarizing elements are referred to as either Polar...

Page 36: ...der Compensators Compensators enhance the intensity of colors in a sample making it easier to identify and differentiate samples Various compensators are available for Primotech microscopes Each incre...

Page 37: ...termine the fracture direction of a material 3 Insert the fixed analyzer into the horizontal slot of the intermediate tube Alternatively insert the rotatable analyzer set to the 0 position into the sl...

Page 38: ...ains a Bertrand lens which can be inserted into the beam path The Bertrand lens causes the interference patterns to be in focus when looking through the eyepieces rather than the sample itself 5 7 Per...

Page 39: ...he condenser to the 0 position 6 Insert the rotatable analyzer into the horizontal slot of the intermediate tube 7 Turn the lever 2 to the left to insert the Bertrand lens into the beam path You can a...

Page 40: ...me of the objective e g Epi plan 5x 0 13 W0 8 contains the following information Series Epi Plan Magnification 5x Numerical aperture 0 13 Screw connection W0 8 Procedure 1 Lower the stage 2 Rotate the...

Page 41: ...also adjust the aperture diaphragm For more information see Adjusting the Resolution and Depth of Field 23 6 3 Changing the Tube Procedure 1 Unplug the cables between the tube and the rear of the mic...

Page 42: ...h with the intermediate tube 4 Rotate the tube 90 clockwise so that the eyepieces are at the front of the microscope Alternatively rotate the tube 90 counter clockwise to save space 5 Use the 2 5 mm h...

Page 43: ...working INFO The reflected illumination light source cannot be changed To change the transmitted illumination light source Procedure 1 Use the 1 5 mm hex key to loosen the two hexagonal bolts of the l...

Page 44: ...g the MNA To remove the MNA Procedure 1 Unplug the Ethernet cables connected to the MNA 2 Use the 2 5 mm hex key to undo the four hexagonal bolts of the MNA 1 2 3 Carefully remove the MNA from the sta...

Page 45: ...Surfaces To clean the external optical surfaces of components Procedure 1 Switch the device off completely and pull the mains plug 2 Remove the component from the microscope or move the stage so that...

Page 46: ...and Preventive Maintenance To maintain the availability and performance of your Primotech system at a fully predictable budget we recommend you enter into a Protect service agreement with your ZEISS r...

Page 47: ...ys place the dust cover over it If it is not going to be used for a longer period of time disassemble and store the microscope Procedure 1 Press the power switch to turn off the microscope 2 Remove an...

Page 48: ...the instrument has no material or production defects when delivered You must inform us of any defects immediately and do everything to minimize any damage If the manufacturer is informed of such a de...

Page 49: ...stand is plugged in see Assembling the Microscope 15 Replace the light source see Replacing the Light Source 43 The field of view is not completely visible Ensure the nosepiece is rotated into a clic...

Page 50: ...he condenser see Specifying the Condenser Position 27 Stage moves downwards by itself Adjust the torque of the focus wheel see Selecting Objectives and Focusing 20 Tube 30 20 with int 3 MP camera Came...

Page 51: ...accordance with DIN EN 61010 1 IEC 61010 1 including CSA and UL regulations Overvoltage category II Radio interference suppression In accordance with EN 55011 Resistance to interference In accordance...

Page 52: ...according to DIN EN 62471 Color temperature 5000 K Homogenous field illumination 20 mm diameter Suitable for objectives with magnification 5x 63x for transmitted light 5x 100x for reflected light Ana...

Page 53: ...al 73 of eyepiece field of view sensor cropped for performance reasons Sensor Micron MT9P031 1 2 5 7 13 mm diag CMOS 24 bit color 2560 x 1920 pixels 2 2 m pixel size Spectral sensitivity without IR fi...

Page 54: ...pieces network adapter not including base plate Approx 192 x 467 x 442 mm incl eyepieces network adapter not including base plate Approx 192 x 467 x 442 mm incl eyepieces network adapter not including...

Page 55: ...be installed and operated in an enclosed space Category Property Value Transport in packaging Permissible ambient temperature 40 to 70 C Storage Permissible ambient temperature 10 to 40 C Permissible...

Page 56: ...dapters Base plate Calibration slide for Matscope App Dust cover to protect the microscope when not in use Zeiss toolkit including 1 5 mm hex key 2 5 mm hex key Documentation Safety information printe...

Page 57: ...filter slider 3200 K d 25 mm 9 Objectives Epiplan 5x 0 13 W0 8 442020 9902 000 Epiplan 20x 0 4 W0 8 442040 9902 000 Epiplan 50x 0 65 W0 8 442060 9902 000 10 Microscope network adapter 430055 9100 000...

Page 58: ...illumination 9 Conversion filter slider 3200 K d 25 mm 10 Objectives Epiplan 5x 0 13 W0 8 442020 9902 000 Epiplan 20x 0 4 W0 8 442040 9902 000 Epiplan 50x 0 65 W0 8 442060 9902 000 11 Microscope netw...

Page 59: ...ider for oblique illumination 8 Conversion filter slider 3200 K d 25 mm Conversion filter 3200 K d 45 mm 9 Analyzer slider D A rotat 360 Polarizer slider A fixed 10 Compensator 6x20 11 Objectives Epip...

Page 60: ...erture diaphragm and polarizer 360 4 Tube 30 20 5 Intermediate tube Bertrand system 6 Microscope stand 7 Analyzer slider D A rotat 360 8 Compensator 6x20 9 Objectives A Plan 5x 0 12 Pol W0 8 441023 99...

Page 61: ...MNA 44 Objectives 40 Tube 41 Cleaning 45 Collisions Preventing 28 Color of reflected light 22 Compensators 36 Components Overview 10 Scope of delivery 56 Condenser Adjusting 27 Focus 29 Height 27 K hl...

Page 62: ...nce By the Customer 45 Corrective and preventative 46 Replacement parts 46 Support 47 Matscope app 25 Maximum height Condenser 27 Microscope Assembling 15 Disassembling 47 Overview 10 Stands 6 Microsc...

Page 63: ...18 Stands 6 56 Storage 47 Support 47 System overview 10 T Technical data Dimensions 54 Environmental requirements 55 Operating Data 51 Power Requirements 51 Specifications 54 Transmitted light Adjust...

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