Perkin-Elmer Lambda 20, Installation And Maintenance Manual, Page: 35 / 108

Lambda 20, Lambda 40 UV/Vis Spectrometers
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When the cell holder has been aligned once, you can take it out and reinstall it
without aligning it again.
Minimum Volume Applications
To measure minimum sample volumes, use microcells (offered by PerkinElmer).
The minimum sample volume required is a function of the cell internal width or
volume and is specified below.
Cell Type Cell
Internal
Width
Pathlength Minimum
Volume
Required
Part
Number
2 mm 1 cm 200 µL B0631071
(pair) Height of
liquid slightly
more than
height of
beam
4 mm 1 cm 400 µL B0631064
(pair)
Cell
Volume
Pathlength Minimum
Volume
Required
Part
Number
0.5 µL 0.01 cm 2 µL B0631082
2.5 µL 0.5 cm 5 µL B0631080
5 µL 0.1 cm 10 µL B0631083
5 µL 1.0 cm 10 µL B0631081
Cell window
completely
filled with
liquid
30 µL 1.0 cm 50 µL B0631079
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