Basic Installation
31
3. Move the milled posts a little to locate the threaded holes in the baseplate, and
then tighten the milled posts.
The tube ports located at the front of the sample compartment allow you to lead
tubes from flowcells, water-thermsotatted cell holders, etc. in and out of the
sample compartment.
When not in use, you should always insert the caps into the ports.
Aligning the Single Cell Holder
Coarse alignment of the single cell holder is carried out as follows:
1. Open the sample compartment cover.
2. Fill matching cells with a low-absorbing solvent (deionized water or ethanol).
3. Insert one cell into the sample cell holder and one into the reference cell holder.
Make certain that the cell is pushed down fully.
NOTE:
The alignment procedure is for a given cell in a given holder. After alignment, the
cell should always be used in the same holder.
4. Block the sample and reference beam window on the right hand side of the
sample compartment with a card to prevent white light from saturating the
detector.
5. Return to standby display.
6. Using the [GOTO] key, slew the monochromator to 0 nm to obtain a beam of
visible (zero order) radiation in the sample compartment.
7. By holding a piece of matt white paper behind each cell holder, visually
examine the light spot to see that the radiation beam is passing through the cell
sample area.
Diffraction patterns become apparent if the radiation beam impinges on the cell
wall.
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Summary of Contents for Lambda 20
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