Mosaic3
51
Version 3.5 rev 8 May 2019
OPERATION
5.6.3 c
entration
of
the
i
LLumination
to
the
m
icroscope
When a laser illumination source is installed, the microscope will have a Fluorescent Target installed in the nosepiece
turret. Rotate the turret to the fluorescent target’s position. In the case of an inverted microscope, remove any slide
carrier or shield such that the entire window of the fluorescent target may be viewed.
If an upright microscope is employed, place a mirror (provided) down on the stage, such that the fluorescent target
window can be viewed. The fluorescent target safely shows the location of the laser illumination beam as it reaches the
nosepiece plane, by converting the laser radiation entirely to non-coherent fluorescence emission.
Figure 18: Laser Spot on Fluorescent Target
Select the appropriate dual illuminator beam splitter plug and wide field illuminator beam splitter plug for your intended
application and install these assemblies in to the Mosaic optical head. Place the provided Mosaic fluorescence filter
cube into the active position.
Configure the Mosaic software to illuminate the entire Mosaic field of view. Set the laser to a low power setting and view
the spot pattern on the target with the laser illuminator source active. An orthogonal grid of 2 to 3 spots across and
down will be visible on the target and one spot will be brighter than the rest. Employ the joystick in the Angle Socket
(Figure 21) in pitch and yaw axes to bring the bright spot to the cross line on the target. This alignment assures that the
laser illumination propagates down the optical axis of the objectives. Turn off the laser.
Because the two adjustments, position (5.6.2) and angle (5.6.3), are not quite independent, it may be necessary to do a
second iteration of these two adjustments for ideal alignment.
5.6.4 s
oftWare
r
egistration
/c
aLibration
The imaging system may need Mosaic registration calibration with the objectives and fluorescence filter cubes desired
for the work planned. This maps the requested Region of Interest drawn for illumination onto the DMD’s pixels to
accurately target the sample.
This will be a function specific to the selected control software, please consult the appropriate User Manual for
guidance.
