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Oxford Instruments ANDOR Mosaic3, Hardware Manual

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Oxford Instruments ANDOR Mosaic3 Hardware Manual Download Page 45

Mosaic3

45

Version 3.5 rev 8 May 2019

OPERATION

5.4.3 m

ask

 r

eferencing

 m

odes

If masks are loaded onto the Mosaic’s on-board memory then there are two ways of referencing which mask to select 
from memory to expose:

Software

A software command from the control PC identifies the required mask for Exposure.

Hardware

The required mask is referenced by an 8 bit binary address input through the D-SUB External Interface Connector.

5.4.4 t

rigger

 m

odes

There are multiple ways to trigger the exposure of an illumination pattern. The method available depends on the 
operational and referencing modes selected.

Mirror On

A software command Exposes the selected mask and leaves it exposed indefinitely.

Mirror Off

A software command turns all of the DMD Mirrors ‘Off’ and leaves them in this state indefinitely.

Expose

A software command Exposes the currently selected mask for the defined exposure time.

Sequence Start

A software command triggers the Mosaic head to execute a predefined sequence of masks.

External Expose

A hardware signal is received via the External Trigger connector (SMA or D SUB) which exposes the currently selected 
mask for the defined Exposure time.
In Sequencing Mode the end of the exposure increments to the next mask, and awaits the next External Trigger to initiate 
its exposure.

External Bulb Mode

A hardware signal is received via the External Trigger connector (SMA or D SUB) which begins to expose the mask on the 
rising edge of the Trigger Signal and ends the exposure on the falling edge.
In Sequencing Mode the falling edge of the of the trigger signal increments to the next mask and awaits the next rising 
edge of the Trigger Signal to initiate its exposure.

External Sequence Start

 

A hardware signal is received via the External Trigger connector (SMA or D SUB) which triggers the Mosaic head to 
execute a predefined sequence of masks.

Compatible Trigger Modes

Operational Modes

Mask 

Reference 

Mode

Mirror On

Mirror Off

Expose

Sequence 
Start

External 
Expose

External 
Bulb

External 
Sequence 
Start

Single Shot Mode

N/A

-

-

Mask 

Memory 

Mode

Referencing

Software

-

-

Hardware

-

-

Sequencing

Software

-

-

*

*

*

Summary of Contents for ANDOR Mosaic3

Page 1: ...Hardware Guide covers Mosaic 3 and Infinity USB 3 0 models Mosaic3 andor com Andor Technology Ltd 2019 Version 3 5 revised 8 May 2019 ...

Page 2: ...TION 2 PRODUCT OVERVIEW 29 2 1 GENERAL 29 2 2 MOSAIC INFINITY CONFIGURATION 30 2 3 OVERVIEW OF DMD ARRAY TECHNOLOGY 31 2 4 SYSTEM COMPONENTS 32 2 4 1 USB 405 nm Laser Controller 32 2 4 2 Laser Homogenizer 32 2 4 3 Light Sources 32 2 4 4 Input Shutters 32 2 4 5 USB 3 0 Cable 32 2 4 6 USB 3 0 Interface PCI Express PCIe Card Optional 32 2 5 SYSTEM OVERVIEW 33 2 6 OPTICAL LAYOUT 34 2 6 1 Mosaic Intern...

Page 3: ...ONTROL 37 2 8 SOFTWARE REQUIREMENTS 37 2 9 INTERLOCK SAFETY SYSTEM 38 SECTION 3 HARDWARE DESCRIPTION 39 3 1 MOSAIC DMD HEAD 39 3 1 1 Power Switch 39 3 1 2 Power 39 3 1 3 DMD Active 39 3 1 4 EXT Trigger 39 3 1 5 External Interface 40 3 1 6 Communications Link 40 3 1 7 Connector Signal Electrical Specification 40 ...

Page 4: ... 42 4 6 MOSAIC OPTICAL FIBRE 42 4 7 SOFTWARE INSTALLATION 42 4 8 REMOTE INTERLOCK 42 4 9 SYSTEM COMPONENTS AND ACCESSORIES 42 SECTION 5 OPERATION 43 5 1 EMERGENCY DISCONNECTION 43 5 2 POWER UP SEQUENCE 43 5 3 POWER DOWN SEQUENCE 43 5 4 USING THE MOSAIC 44 5 4 1 Control 44 5 4 2 Operational Mode 44 5 4 3 Mask Referencing Modes 45 5 4 4 Trigger Modes 45 5 4 5 Invert Mode 46 5 4 6 Sequencing 46 ...

Page 5: ...on Power Density Control 49 5 6 2 Centration of the Laser Illumination on the DMD 50 5 6 3 Centration of the Illumination to the Microscope 51 5 6 4 Software Registration Calibration 51 5 7 CHANGING THE DICHROIC POSITION MOSAIC INFINITY 52 5 8 RISK MITIGATION 52 5 8 1 Interlock Safety System Testing 52 5 8 2 Mechanical Housing 52 SECTION 6 MAINTENANCE 53 6 1 OPTICAL ELEMENTS 53 6 2 REGULAR CHECKS ...

Page 6: ... SUPPLY REQUIREMENTS 57 8 2 POWER CONSUMPTION 57 8 3 ELECTRICAL SPECIFICATIONS I O 57 8 4 OPERATING CONDITIONS 58 8 5 STORAGE AND TRANSPORTATION 58 8 6 PERFORMANCE SPECIFICATIONS 58 8 7 LASER SOURCES 58 APPENDIX A MECHANICAL DRAWINGS 59 APPENDIX B GLOSSARY 61 APPENDIX C OTHER INFORMATION 62 ...

Page 7: ... and updated information to match latest Mosaic3 model features and functionality all sections 3 1 11 Feb 2015 Updated to include to cover the Mosaic Infinity models 3 2 18 Feb 2016 Updated to cover USB 3 0 functionality 3 3 09 Mar 2016 Updated power consumption values Section 8 2 3 4 27 Sep 2016 Corrected DMD image diagram Section 2 3 Changed references of active illumination to Photostimulation ...

Page 8: ...anufactured for the medical diagnosis of patients 10 Do not attempt to bypass any system safety interlocks They are provided to comply with the safety requirements of various regulatory agencies and must be employed to protect the operator 11 Protective earth is an integral part of the protection against electric shock in this product and is provided via the earth pin of the external power supply ...

Page 9: ...ation should provide their own protection 21 Your product is a precision scientific instrument containing fragile components Always handle it with care 22 Ensure fibre cables are not bent tightly as this may damage the internal optical fibre For transport or storage fibre cables should be coiled with a diameter of 300mm or greater 23 If noise patterning or optical fluctuations are seen contact And...

Page 10: ...ble and that this laser product is not fully safe as you usually have access to hazardous laser radiation Laser safety for this type of product is about reducing risk rather than being able to eliminate it because access is required for the performance of this product It is important to remember to beware reflections from objects such as tools or clips placed close to the laser beam emitted from t...

Page 11: ...properties of lasers especially that laser safety hazards differ from those of normal light sources as they are highly concentrated very low divergence beams of radiation 3 Ensure the equipment is used in a controlled area by trained end users in accordance with national guidelines 4 Ensure end users are familiar with the operation of the laser s key switch control interlocks emission LEDs and oth...

Page 12: ...d indicate the output of invisible wavelengths at all times 9 Additional precautions may need to be implemented as the necessary precautions will be specific to each system installation configuration and typical mode of use The responsible Laser Safety Officer must assess and implement the necessary precautions to avoid possible exposure to hazardous radiation during use Eye Protection Eye Protect...

Page 13: ...his product meets the requirements of the applicable CE marking directives of the EU 3rd Party Products The laser sources incorporated in this product are supplied from 3rd party suppliers Reproductions of their labels and their positions on these laser sources are included in their manuals which are supplied along with this user guide These labels will include laser powers that are only output di...

Page 14: ...at distances less than 13 cm between the diffusing surface and the cornea can exceed the MPE The following terms are defined as follows Intrabeam ocular exposure Getting your eye in the way of a laser beam Nominal Ocular Hazard Distance NOHD Distance from the output aperture beyond which the beam irradiance or radiant exposure remains below the appropriate corneal maximum permissible exposure MPE ...

Page 15: ...must be additionally labelled The maximum optical power stated is the worst case possible and will normally be greater than the maximum actually accessible There are many different versions of this label due to the product variants but the following are two examples The acronym CW refers to Continuous Wave and means that the laser can output the power shown continuously as opposed to the case of p...

Page 16: ...d from an aperture either deliberately at the microscope objective or potentially as a result of disassembly during installation or servicing International Laser Aperture Labels Microscope Objective Output This is located at the nearest practical point to the actual emission Potential Laser Apperture Label No 1 Laser Diode Module Output ...

Page 17: ...Mosaic3 17 Version 3 5 rev 8 May 2019 Potential Laser Aperture Label No 2 Covered Uncovered Adalaser Output Potential Laser Aperture Label No 3 Homogeniser Optical Fibre Output ...

Page 18: ...18 Version 3 5 rev 8 May 2019 Potential Laser Aperture Label No 4 Covered Uncovered Fibre Collimator Output This is the part that through which laser radiation from the optical fibre enters the main Mosaic unit ...

Page 19: ...ser Aperture Label No 5 Standard Epi Port Adaptor Output The standard or non infinity versions of Mosaic connect to the microscope via its epi port and have a potential laser aperture if installers service personnel disconnect the system at this point ...

Page 20: ...l Laser Aperture Label No 6 Infinity Microscope Adaptor Lower Transmission Port There is a potential laser aperture where the Infinity versions of Mosaic connect to the microscope if installers service personnel disconnect the system at this point ...

Page 21: ...to open these and only service personnel will need to gain access but the laser standards direct us to warn you of access using the following labels Access Panel Label No 1 Fibre Collimator Adjustment Slots When the cover is removed from the Fibre Collimator where the fibre enters the main unit the screws for illumination control are exposed As tools could accidentally access laser light there is ...

Page 22: ...2 Version 3 5 rev 8 May 2019 Access Panel Label No 2 Optical Body Light Input Block Interlock Cover There is a tool accessible panel to allow access to an interlock for installation service personnel as follows ...

Page 23: ...Mosaic3 23 Version 3 5 rev 8 May 2019 Access Panel Label No 3 Optical Body Light Input Block Secondary Port Blanking Plate Access Panel Label No 4 Optical Body Focus Adjustment ...

Page 24: ...ptor Upper Emission Port There is a potential access point where the Infinity versions of Mosaic connect to the microscope if installers service personnel disconnect the system at this point CDRH Certification Label This self certifies that we comply with the U S CDRH laser regulations ...

Page 25: ... executed with Hard Real Time accuracy under software control or following external Trigger signals for complete experimental flexibility Figure 1 The Mosaic Mosaic 3 It has the following features Supports a range of light sources such as LED laser and arc to match a wide range of techniques from optogenetics to photobleaching Unlimited flexibility in shape size and complexity within the 800 x 600...

Page 26: ...28 9023 7126 Fax 44 0 28 9031 0792 Andor Technology 300 Baker Avenue Suite 150 Concord MA 01742 USA Tel 1 860 290 9211 Fax 1 860 290 9566 Asia Pacific China Andor Technology Japan 5F IS Building 3 32 42 Higashi Shinagawa Tokyo 140 0002 Japan Tel 81 0 3 6732 8968 Fax 81 0 3 6732 8939 Andor Technology Unit 1 Building A 66 Zhufang Rd Haidian Dist Beijing 100085 China Tel 86 0 10 8271 9066 Fax 86 0 10...

Page 27: ...gs are the property of Andor Technology Ltd and all rights are reserved This document and the associated drawings are issued on condition that they are not copied reprinted or reproduced nor their contents disclosed The publication of information in this documentation does not imply freedom from any patent or proprietary right of Andor Technology Ltd or any third party 1 4 Trademarks and Patent In...

Page 28: ...plied with your system and inspect against the components specified at time of ordering 1 6 Optional Components There are a range of optional components available for the Mosaic 3 Please contact your nearest Andor Sales Representative for further information on these and other system components available ...

Page 29: ...the laser source if one is present Laser Zoom Collimator This expands and collimates the input laser beam to match it to the DMD Array Flange for Second Light Source An optional second light source may be connected e g LED source second laser source halogen lamp This can allow illumination with two different wavelengths simultaneously or fast switching by hardware control of the light sources Flan...

Page 30: ... mosaic to be integrated into an upright microscopy set up The features and functionality of the infinity version are the same as the Mosaic 3 Section 2 1 apart from as detailed in the following sections Figure 3 The Mosaic Infinity for upright microscopes Dichroic Slider Enables selection between three positions Dichroic Slider ...

Page 31: ...of a small area of a DMD Mirror array face on view ON Mirror 10 OFF Mirror 10 Tilt Axis Incident light 90 to Tilt axis Reflected light from ON Mirror coming out of page Reflected light from OFF Mirror going to the right In the DMD power off state the mirrors lie flat at 0 to the beam direction When any mirror is switched ON the tilt angle is 10 to this flat state and 10 in the OFF powered on state...

Page 32: ...tative for any compatibility queries 2 4 4 Input Shutters The Mosaic may be supplied with one or more fast mechanical shutters on the light source inputs which are used to blank the light input for increased extinction ratio Please refer to the User Manual supplied with the device for operation of the Shutter Driver unit A shutter will be supplied with any laser based system and acts as a User Con...

Page 33: ...a Mosaic 3 system Camera Eyepieces Diode Laser Controller Light Source Lamp house tube Beam Splitter Plug Camera Port Safety Interlock EPI port Shutter Controller Homogenizer Light Source Microscope Computer DMD Head Mosaic3 Optical Body Fibre Fibre Collimator Fibre Coupler 405 Diode Laser for example Lamp house tube PS 12 EPS Shutter USB RS232 USB etc 12V USB 3 0 cable USB 3 0 Dual Lamp House Blo...

Page 34: ...Light Paths There are two main variants of the Mosaic device 1 Standard Single Input Block 2 Duet Dual Input Blocks The Internal Light Paths in this example for Mosaic 3 are shown below Figure 7 Internal light path of Mosaic 3 Standard version Figure 8 Internal light path of Mosaic 3 Duet version ...

Page 35: ...osaic3 35 Version 3 5 rev 8 May 2019 PRODUCT OVERVIEW 2 6 2 System Light path Upright Microscope An example for an upright microscope is shown below Figure 9 Upright Microscope light path Mosaic Infinity ...

Page 36: ...esignated aperture The laser is coupled into the Mosaic via an armoured fibre optic cable This fibre optic forms part of the protective housing and care should be taken not to pull or damage the fibre The fibre uses FC couplings the connection points are shielded as the fibre is not user removable Camera Mosaic3 Eyepiece Interlock Sensor Activated Laser Excitation Light and or Widefield Light Fluo...

Page 37: ...ice an SDK is available for system integrators or advanced users please contact your Andor Sales Representative to apply for access 2 8 Software Requirements To control the Mosaic you will need one of the following software packages and a Windows based PC Andor iQ 3 0 onwards previous versions compatible but offer limited functionality Molecular Devices MetaMorph 7 7 1 onwards and NX 2 5 275 0 In ...

Page 38: ...detect the state and configuration of the Microscopy System including the Microscope Eyepiece prism the transmitted light arm on inverted microscopes and the presence of a stage cover if one is installed Additionally the Beam Splitter Dichroic plugs are interlocked This is designed to prevent the user from being accidentally exposed to the potentially high level of laser radiation emitted by the M...

Page 39: ...e switch will be illuminated 3 1 2 Power The AC DC external power supply input supplies the Head with 12V power 3 1 3 DMD Active This output is asserted high when the Mosaic is displaying an image on its mirrors and not global dark all mirrors off and is supplied via an SMA coaxial connector This may be asserted but an image may not be displayed if no light source is illuminating the DMD mirrors 3...

Page 40: ...to external triggering DMD Active and Ext Trigger signals are copies of the signals mentioned above and perform the same functions 3 1 6 Communications Link This connects the Mosaic DMD Head to the PC using a USB 3 0 cable 3 1 7 Connector Signal Electrical Specification Connector Connector Type Signal Signal Type Specification DMD Active SMA Digital Output VIH 2 0 V across a 50 Ω load Can drive 50...

Page 41: ...peration 4 3 Assembly This product itself requires no assembly and will be installed by an Andor Technician There are no user serviceable parts 4 4 Power Connection and Protective Earthing See Section 5 1 Emergency Disconnection Before connection check that the mains power socket used can provide the power as specified in Section 7 1 Power Supply Always switch off power before connecting disconnec...

Page 42: ...nical Support 4 7 Software Installation If the device is supplied with Andor s iQ Software then the Installation Technician will ensure that the software is installed and operating correctly For all other software programs please see the applicable User Guide for details Future upgrades can be installed by the customer The latest Andor software releases are available through MyAndor http my andor ...

Page 43: ...s fully upright position 2 Power on the workstation 3 Power on the Mosaic the rocker switch shall illuminate green 4 Start the control software program and ensure that it has connected to the device successfully 5 Ensure that the USB laser controller is turned Off if in Manual control mode or if under Software Control ensure that it is set to Zero power 6 Power on the homogenizer 7 Power on all li...

Page 44: ...0 fps dependent on the workstation s capabilities 2 Mask Memory Mode Up to 139 masks are uploaded to the device s on board memory and then are referenced and exposed individually or in a defined sequence The masks their exposure specification and any sequences need to be generated in advance and uploaded to the device This allows the fastest control of masks and sequences as it is under full Hardw...

Page 45: ...to execute a predefined sequence of masks External Expose A hardware signal is received via the External Trigger connector SMA or D SUB which exposes the currently selected mask for the defined Exposure time In Sequencing Mode the end of the exposure increments to the next mask and awaits the next External Trigger to initiate its exposure External Bulb Mode A hardware signal is received via the Ex...

Page 46: ... applicable User Manual for guidance The following shows how the sequences are implemented internally with the Mosaic The Masks are loaded into the Mask Memory The sequence Memory is populated as a series of entries up to 1023 entries each can specify one or more consecutive masks to display for a specified period and up to 65536 repetitions A sequence command specifies one or more consecutive Seq...

Page 47: ...s 3 µs after the DMD finishes exposing 5 4 11 Beam Splitter Plug Operation The beam splitters are each contained in an interchangeable kinematic type mount that we call a Beam Splitter Plug A Beam Splitter Plug can contain optical elements to Completely block transmission of light from the primary light source while allowing all the light from the secondary source to pass into the microscope Provi...

Page 48: ...the lamp house filter grip the holder and pull upwards as shown in figure 17 below Figure 14 Heat Shield Filter Warnings The system should not be operated without a filter holder in position The Lamp House Filter should not be removed while the Mosaic is in operation Before changing the Lamp House Filter ensure that the laser and lamp are turned off Do not insert any objects into the Filter s Aper...

Page 49: ...sh to alter the size of the illumination spot and therefore power density of the laser onto the DMD 5 6 1 Illumination Magnification Power Density Control The laser illuminator subsystem fibre collimator is configured to illuminate a nearly circular spot on the DMD that corresponds to the user s desired work area For users that wish to use the highest power densities the area should be as small as...

Page 50: ... used with the Mosaic and focus on the fluorescence sample provided Select the provided Mosaic fluorescence filter cube and imaging camera port for viewing This will engage the eyepiece laser interlock and allow the laser to turn on when requested Configure the Mosaic software or software toolbar installed in the imaging system refer to supplied software guide to illuminate the entire Mosaic field...

Page 51: ...c fluorescence filter cube into the active position Configure the Mosaic software to illuminate the entire Mosaic field of view Set the laser to a low power setting and view the spot pattern on the target with the laser illuminator source active An orthogonal grid of 2 to 3 spots across and down will be visible on the target and one spot will be brighter than the rest Employ the joystick in the An...

Page 52: ... Testing If a laser system is present then the operation of the Interlock safety system should be tested prior to use of the system When the system is operational partially remove a Beam Splitter plug and ensure that the Emission LED on the front of the diode lasers controller extinguishes If a fault is ever observed please report it immediately to your local Andor Support representative and stop ...

Page 53: ...l be given by the Andor Installation Technician No other optical elements are user serviceable Only attempt to clean if particulates are causing visible non uniformities in the illumination field Visually inspect the optic and do not attempt cleaning if particulates are not visible to the naked eye Use disposable optical grade swabs or lint free tissue and an optical solvent Work with single passe...

Page 54: ...instructions outlined below WARNINGS Turn off lasers before beginning this procedure Take care handling optical components following standard practices for good optical handling techniques 1 Ensure that lasers are powered off 2 Remove the 4 M3x8mm screws on the access panel on the dichroic module shown below 3 Remove the 4 M3x12mm screws which secure the required dichroic mount to the dichroic sli...

Page 55: ...OOTING SECTION 7 TROUBLESHOOTING 7 1 Troubleshooting Examples Refer also to the operation and troubleshooting information provided with your other system components Contact your Andor customer support representative if you require further assistance ...

Page 56: ...Please have the following information when connecting customer support Owner Information Institution Name Vendor Information Vendor Contact Equipment Information Model Serial No Check List Cable Connections Mains Power Summary What is the nature of the problem Any other observations ...

Page 57: ... Voltage Output Connector Tyco 3 1437719 3 4 pin Triad Female Cable Plug which uses UL 94 V 0 insulators Pins are shown looking into the connector from the insertion side General Safety Requirements The power supply must be certified to an appropriate IEC standard e g IEC 60950 1 and meet the reinforced insulation from mains requirement of IEC 61010 1 Power Supply Overvoltage Category CAT II An ov...

Page 58: ...20 to 70 C Relative Humidity 90 non condensing 8 6 Performance Specifications Parameter Specification Potential Wavelength Range 360 nm to 800 nm minimum 5 at all wavelengths Laser Wavelengths See laser source s labels and or explanatory label on microscope Maximum Power at Microscope Stage See explanatory label on microscope Intensity Stability Absolute dependent on illumination source Extinction...

Page 59: ...Mosaic3 59 Version 3 5 rev 8 May 2019 MECHANICAL DRAWINGS APPENDIX A MECHANICAL DRAWINGS All Dimensions mm inches Mosaic 3 Mosaic 3 Duet Mosaic 3 Head all models ...

Page 60: ...Mosaic3 60 Version 3 5 rev 8 May 2019 MECHANICAL DRAWINGS Mosaic Infinity 1 0X Mosaic Infinity 0 5X ...

Page 61: ...rol microscopy systems LED Light Emitting Diode MEMS Micro Electro Mechanical Systems Devices made up of components between 1 to 100 micrometres microns in size Optogenetics A neuromodulation technique employed in neuroscience that uses a combination of techniques from optics and genetics to control the activity of individual neurons in living tissue Optophysiology The study of the response of bio...

Page 62: ...conditions of sale including warranty conditions will have been made available during the ordering process The current version may be viewed at www andor com pdfs literature Andor_Standard_Warranty pdf Waste Electronic and Electrical Equipment Regulations 2006 WEEE The company s statement on the disposal of WEEE can be found in the Terms and Conditions ...

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