Moxi Flow
™
User Guide
Page 27
Cell clumping
Ensure the cells are in a single-cell suspension.
Break clumps by pipetting up and down with a
standard pipette. Protease treatment can also be
used to dissociate clusters. ORFLO recommends
Accutase or equivalent.
Improper gating
Size measurements are reported as the mean
size for a gated range. If a cell population is not
properly gated, contributions of other cell types,
system noise, or debris particles can contribute to
the mean size calculation.
No clear histogram peak on size
histogram
Improper scaling
This is usually associated with very-low-
concentration samples. For histogram overlay
use finger swipes (left - right) for the fluorescence
histogram and (top – bottom) for the size
histogram. For size histograms (not overlaid),
use the white (black box background) arrows to
adjust the vertical scale to properly scale the
population.
Small Particles
A smaller particle population might have a left tail
that extends into the system noise (below the
specified range of the cassette). Re-scaling the
x-axis (can only be done after a test, prior to
saving) can increase the binning resolution
around these populations, helping to better view
the peak.
Debris-filled sample or size-
overlapping cell samples
(polydisperse samples).
Overlapping size populations can obscure the
downward slopes of cell peaks. Often debris
populations can obscure the left side of a cell
peak.
Overly Long Test Times
Insufficient fluid (<75
µ
L)
Adjust pipette to ensure sufficient volume for the
test. For improperly calibrated pipettes, this
might require a setting >75
µ
L
Cells/beads too large
Make certain that the particle type is within the
specified size range of the cassettes being used
Highly/strongly aggregated cells
clogging filter and blocking flow
Try breaking apart cells using pipette trituration
and/or protease treatment.
Red Screen – “Software
Exception”
System firmware has detected a
malfunction.
Please take a photo and send to
[email protected]. Reset the instrument
by pressing and holding the power button for at
least 5 seconds.
System shuts off during
alignment/test
System is unplugged
Plug the system in to the AC wall adapter and re-
try.
