Moxi Flow
™
User Guide
Page 20
are divided into upper and lower regions based on the fluorescent gating (red gates).
These regions are labeled appropriately for the tests:
•
Live Cells and Dead Cells for the “Viability Assay”
•
Apoptotic and Non-Apoptotic for the “Apoptosis Assay”
•
Lower Population and Upper Population for the “Open Flow Cytometry” and “Fluorescence
Bead Assay”
The particle concentration for each region, as well as the mean effective diameter for each
region, is displayed in separate labeled black boxes beneath the data plot.
Note: This is an
“effective” diameter that is based on a precise volumetric measurement (Coulter Principle)
combined with a spherical assumption.
The total particle population within the size (blue) gates
is displayed at the top of the data plot. The ratio of region of interest to the total size-gated
population is displayed in the yellow box at the top of the data plot. For “Size Histogram” tests,
the black boxes provide the gated particle concentration (left black box beneath the histogram,
see right image on the bottom of page 19) and effective mean particle diameter and mean
particle volumes (right black box beneath the histogram). For “Size Histogram” tests, an MPI
value is also provided in the yellow box at the top right of the display. This value is a measure
of the purity of the sample by providing a ratio of the Curve Fit or Gated population to the total
particle population (not including the automatically grayed out noise region). This metric has
shown utility in tracking culture health by highlighting increases in small particle components
that inevitably result from cell necrosis or microbial growth.
File/Test Operations
Test Naming and Renaming Tests
By default, tests are named with an automated prefix based on the test type (“Via” for Viability
Counts, “Open” for Open Flow Cytometry, “Bead” for Fluorescent Bead Assay, “Apop” for
Apoptosis, and “Size” for Size Histogram). Files within test types are auto-enumerated by the
system (e.g. “Via-001”, “Via-002”,
…
) with the lowest available number. Tests can be renamed
at any point (immediately after the run or through editing a saved test) by touching the green
test name box at the top of the scatter plot or histogram (see image below/left). The default
name is presented and can be deleted by hitting the “Clear” key or successive “Backspace”
keys. Capital letters can be achieved by hitting the “ABC” button. Symbols and special
characters can be accessed by hitting the “ABC” button first, followed by the “?123” button.
Select
Save
to assign the newly entered name to the test.
