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Moxi Flow
™
User Guide
Page 6
Part
Function
USB Cable
Connects instrument to PC/Mac or power adapter
Power Adapter, US Style
Connects USB cable to a 110 VAC outlet
(US kit only)
Power Adaptor, UK Style
Connects USB cable to a 230 VAC outlet
(UK kit only)
Power Adaptor, EU Style
Connects USB cable to a 220 VAC outlet
(EU kit only)
Getting Started
The Moxi Flow
TM
is shipped in a condition ready for initial use. To begin, plug the USB Cable
into the instrument at one end (mini-USB). Plug the opposite end of the USB Cable (standard
USB) into the Power Adapter. Plug the Power Adapter into an AC outlet that matches the rating
of the Power Adapter.
CAUTION
Use only an Orflo supplied USB Cable and Power Adapter. Use of other
products may result in inaccurate test results or damage to the
instrument.
NOTE
Tests should only be performed with the instrument powered by an AC
source. Although the instrument has an internal, rechargeable battery,
Orflo strongly recommends that tests not be run on battery power alone.
General Guidelines
The Moxi Flow
TM
system is used with Moxi Type MF-M and Type MF-S Cassettes. Refer to the
Specifications section for information on the operating size and concentration ranges for the
system.
The sample volume for a test should be 75
µ
L.
Cells should be suspended in 0.9% salt solution (e.g. PBS or equivalent) to ensure proper
conductivity for unit operation and to ensure proper particle sizing. Water, hypotonic, or
hypertonic solutions are
not
acceptable diluents.
Cells need to be prepared as single-cell suspensions. Clusters/aggregates should be broken
apart with mechanical trituration and/or protease dissociation (e.g., Accutase - MXA020)
As the Moxi Flow™ uses a 532nm laser with a 590/40nm detection filter, dye/fluorophore