Olympus IX51 Instructions Manual Download | Manualshive

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IX71/IX51

IMPORTANT

This microscope employs a UIS2/UIS (Universal Infinity System) optical design, and should be used with
modules designed for the IX2 or BX2 series (as well as some of the modules designed for the Olympus
IX or BX series).
For the applicable modules, please consult Olympus or the latest catalogues. Less than optimum per-
formance may result if inappropriate module combinations are used.
If you want to use one or more motorized modules, you should prepare the IX2-UCB control box, U-
HSTR2 hand switch, a PC and the IX2-BSW (Ver. 01.03) software (which runs on Windows 2000 or Win-
dows Me).

Configuration of Instruction Manuals

Since these microscopes are expandable to a variety of systems, separate instruction manuals are prepared
so that the user has to read only the manuals according to the user’s own system.

Manual Name

Main Contents

IX71/IX51

Observation procedures including transmitted light brightfield,
phase contrast and DIC observations

Fluorescence System for IX2

Reflected light fluorescence observation

IX2-GCP

Glass Center Plate

IX2-MLWCD

Mid-Long Working Distance Condenser

IX2-DICD

DIC Condenser

U-FWT/FWR/FWO

Motorized filter wheels (The U-FWT cannot be used with these
microscopes.)

IX2-UCB/U-HSTR2

Functions of the Control Box (incorporating the power supply)
and Hand Switch

IX2 Software for PC (CD-ROM)
IX2-BSW (Ver. 01.03 or later)

Methods of PC control of microscope functions

Precautions When Unpacking the Microscope

Releasing the Transport Lock of the Revolving Nosepiece

# Never attempt to rotate the coarse or fine adjustment knob @ without

removing the clamping rod. Otherwise, the focusing mechanism may
be damaged.

1. Loosen the screw ³ of the clamping rod ² using the Allen screwdriver

provided with the microscope frame.

2. Rotate the coarse and fine adjustment knobs @ in the direction of the

arrow and remove the clamping rod ².

3. Attach the provided seal (10 mm dia., black) on the hole made after remov-

ing the transport lock knob to prevent penetration of dust through the hole.

}Retain the clamping rod ² and screw ³ carefully because they will be

used again the next time the microscope is transported.

Releasing the Transport Lock of the Light Path Selector

# Never attempt to operate the light path selector without removing the

transport lock knob |. Otherwise, the light path selector mechanism
may be damaged.

· Rotate the knob counterclockwise to remove it.
· To prevent penetration of dust through the hole made after removing the

transport lock knob, stop the hole by attaching the provided seal (10 mm
dia., black).

}Retain the knob carefully because it will be used again the next time the

microscope is transported.

Stage (IX2-SFR, IX-MVR)

· Before transporting the stage, fix the flexible knobs with pieces of

adhesive tape so that they will not move.

Fig. 1

@

²

³

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4
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Summary of Contents for IX51

Page 1: ...verted Microscopes Models IX71 and IX51 To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of the microscope we recommend that you study this manual thoroughly before operating the microscope Retain this instruction manual in an easily accessible place near the work desk for future reference ...

Page 2: ......

Page 3: ...et U BI90CT Only 7 Adjusting the Tilt U TBI90 Only 1 Rotation Direction of the Coarse Fine Adjustment Knobs 2 Adjusting the Coarse Adjustment Knob Tension 3 Detaching the Fine Adjustment Knob 4 Pre focusing Lever 1 4 1 Placing the Specimen 2 Moving the Specimen 3 Connecting the Grounding Wire 4 Adjusting the X Axis Y Axis Knob Rotation Tension 4 7 Condenser 27 29 1 Centering the Condenser 2 Using ...

Page 4: ...FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential ar...

Page 5: ...upply and Hand Switch IX2 Software for PC CD ROM IX2 BSW Ver 01 03 or later Methods of PC control of microscope functions Precautions When Unpacking the Microscope Releasing the Transport Lock of the Revolving Nosepiece Never attempt to rotate the coarse or fine adjustment knob without removing the clamping rod Otherwise the focusing mechanism may be damaged 1 Loosen the screw of the clamping rod ...

Page 6: ... any consequence of user modification including introduction of the use of laser beam 5 The surfaces of the lamp housing will become extremely hot during operation When installing the microscope make sure to allow ample free space 10 cm or more around and in particular above the lamp housing 6 When installing the microscope route the power cord away from the lamp housing Should the power cord come...

Page 7: ...U EPA2 eye point adjuster With the combination of U TR30H U FWO the full optical performance may not be able to be manifested with the objectives listed in below and it is not possible to use the objectives listed in below This also applies to objectives other than Series 3 objectives UIS2 Series 1UPlanSApo4X 10X2 UPlanFLN4X 10X2 20X CPlanFLN10X LUCPlanFLN20X UPlanSApo100XO 2PlanN40X PlanApoN60XO ...

Page 8: ...ially available absolute alcohol Since the absolute alcohol is highly flammable it must be handled carefully Be sure to keep it away from open flames or potential sources of electrical sparks for example electrical equipment that is being switched on or off Also remember to always use it only in a well ventilated room 2 Be sure to clean the oil immersion objective after use Leaving immersion oil o...

Page 9: ...r with IX MVR IX2 KSP Can be used together with CK40 MVR Eyepieces WHN10X WHN10X H Observation Tube U BI90CT U BI90 U TBI90 U TR30H 2 Used together with IX ATU U MO 6 Position Revolving Nosepiece Fixed on the microscope frame Microscope Frame IX71S1F 3 IX71S8F 3 IX51S1F 3 IX51S8F 3 Fluorescence Mirror Unit Cassette IX2 RFACA IX2 RFAC Hand Switch U HSTR2 Transmitted Light Lamp Housing U LH100L 3 U ...

Page 10: ...ent knob tension adjustment ring Page 16 IX71 Filter pocket Page 23 Field iris diaphragm lever Condenser centering knobs Aperture iris diaphragm lever Indicator plate pockets Option button Page 16 Light intensity control knob Page 14 Transmitted light ON OFF button Page 14 Fine adjustment knob Page 17 Detachable Universal Condenser IX2 LWUCD Light path selector lever Page 15 Observation Side port ...

Page 11: ...ent knob Page 17 Detachable Phase Contrast Slider IX2 SL Applicable halogen bulb 6V30WHAL Lamp Socket U LS30 3 Y axis knob Page 19 X axis knob Page 19 Light intensity control knob Page 14 Main switch Page 14 Power Supply Unit TL4 Light path selector lever Page 15 Observation Side port Binocular Observation Tube U BI90 Microscope Frame IX51S1F 3 IX51S8F 3 Illumination Column IX2 ILL30 Plain Stage I...

Page 12: ... adjustment knob Page 17 Detachable Pre focusing lever Page 17 Eyepieces WHN10X WHN10X H Binocular Observation Tube U BI90CT Allen wrench and optical element centering knob x 2 storage position Rear panel Binocular Observation Tube U BI90CT Allen screwdriver storage position Rear panel Side port Transport lock knob Be sure to remove this before use Diopter adjustment ring Page 20 Interpupillary di...

Page 13: ...crew holes CK40 MVR CK2 SS mounting screw holes Plain Stage IX2 SP IX MVR CK2 SS mounting screw holes On both sides Manipulator mounting screw holes M6 screws x 6 Cross Stage IX SVL2 Y axis knob Can be mounted on the IX2 KSP Y axis knob X axis knob Can be mounted on the IX2 SP Y axis knob X axis knob Intermediate Tube IX ATU Mechanical Stage CK40 MVR Mechanical Stage IX MVR M6 screws x 6 On both s...

Page 14: ...Centering knob insertion hole UCD Adapter IX ADUCD Condenser height fine adjustment knob Page 28 This is a manually controlled condenser Refer to the separate instruction manual for details Motorized Long WD Universal Condenser IX2 LWUCDA2 This is a motorized condenser Index mounting holes Aperture iris diaphragm lever Page 27 Centering knob insertion holes Aperture iris diaphragm lever Page 27 In...

Page 15: ...control knob POWER LED REMOTE LED Hand Switch TH4 HS The IX71 does not need this module because its functions are incorporated in the microscope frame Light intensity control knob Lamp ON OFF switch Power Supply Unit TL4 The applicable halogen bulb is the 6V30WHAL Light intensity control knob Lamp socket connector Main switch ON OFF Main switch ON OFF ...

Page 16: ...with the light path selector lever ƒLight path selector lever P 15 Place the specimen on the stage X axis and Y axis knobs P 19 Engage a 10X objective in the light path Revolving nosepiece Bring the specimen into focus Coarse fine adjustment knobs P 16 Adjust the brightness Light intensity control knob P 14 Adjust the interpupillary distance Adjust the diopter Adjust the light axis ŠBinocular obse...

Page 17: ... IX51 With the IX71 Power Supply Unit TH4 Hand Switch TH4 HS With the IX51 When the TL4 power supply unit is used Make a photocopy of the observation procedure pages and post it near your microscope ƒ Š Œ š ƒ ...

Page 18: ...entical function With the TL4 the position marked is not provided and even the maximum light intensity 6 V may sometimes be unsuitable for photomicrography With the IX71 Frame Fig 6 If the TH4 connection cable provided with the microscope frame is connected to the TH4 the REMOTE LED lights in this case the light intensity control knob on the power supply unit is defeated and only the light intensi...

Page 19: ...0 light path with the IX71S1F 2 IX51S1F 2 frame Side port light path 80 Observation 20 light path with the IX71S8F 2 IX51S8F 2 The light path to the lower back port can also be set by the manufacturer s part replacement operation IX2 LBPC When the magnification selector knob is pulled out the magnification will be 1 6X When the knob is pushed in the magnification will be 1X The 1X 1 6X magnificati...

Page 20: ...ont in the direction of the arrow raises the objective and toward the rear opposite direction lowers the objective Always use the rotation tension adjustment ring to control the rotation tension of the coarse adjustment knob The tension of the coarse adjustment knob has been pre adjusted to optimum tension but this can be changed as required Turn the rotation tension adjustment ring in the directi...

Page 21: ...pproximate focus with the coarse adjustment knob turn the pre focusing lever in the direction of the arrow to lock it Hereafter the upper limit of the coarse adjustment will be limited at the position where the lever is locked When bringing a specimen in focus approximate focus can be obtained by simply raising the coarse adjustment to the stop position so all you have to do more is control the fi...

Page 22: ... plates 72 well or 60 well When using this it is necessary to replace the stage scales with those provided with the plate holder Petri dish holder is available for 35 mm 54 mm and 65 mm diameter petri dishes and a slide glass holder is available for holding slide glass and the IX2 BCTP ƒ is available for a blood cell test plate holder Fig 15 A blood cell test plate or other calculating chamber for...

Page 23: ...age travel area is 130 mm X axis x 85 mm Y axis with the IX MVR or 120 mm X axis x 78 mm Y axis with the CK40 MVR 3 Connecting the Grounding Wire Fig 18 With the IX2 SFR or IX SVL2 Stage A grounding wire can be attached to the stage for electrophysiological experiments etc Prepare a grounding wire and one M4 screw and attach the grounding wire as shown in Fig 18 The screw hole may sometimes be stu...

Page 24: ...ld of view is clearly visible Fig 21 The diopter can also be adjusted using the eyepiece micrometer disks 2 While looking through the right eyepiece rotate the coarse fine adjustment knobs to bring the specimen into focus 3 Look through the left eyepiece and rotate only the diopter adjustment ring on the left eyepiece to bring the specimen into focus Fig 21 When rotating the diopter adjustment rin...

Page 25: ...gaged in the light path and the objective exit pupil can be observed Used when centering the ring slit in phase contrast ob servation or adjusting the aperture iris diaphragm S Shield plate is engaged in the light path to block extraneous light from entering through the eyepieces Used in photomicro graphy to prevent extraneous light from affecting the exposure 2 To bring the exit pupil image of th...

Page 26: ...can be created by tilting the illumination column Even with the illumination column tilted the specimen surface will be illuminated which is convenient for rough confirmation of the specimen location or initial positioning when placing the specimen 1 Using the Allen screwdriver loosen the column tilt clamping screw by turning it approximately 11 turns in the direction of the arrow 2 Holding the il...

Page 27: ... 31 2 While holding the mounting lever of the filter holder insert a filter Fig 30 Hold the filter by its edge to avoid leaving fingerprints or smudges on the filter surfaces After the illumination has been ignited the filter will be very hot Be sure to set the main switch to OFF and allow the filter holder and filters to cool down before replacing filters 3 Engage each filter in the light path by...

Page 28: ...ig 34 2 Insert the Allen screwdriver into the hole on the condenser height adjustment knob and while holding the knob turn the screwdriver in the direction of the arrow to increase the rotation tension of the knob or in the opposite direction to decrease the tension 3 After adjustment tighten the two clamping screws 1 securely Changing the Knob Position The condenser height adjustment knob is deta...

Page 29: ...hragm can be seen as shown in Fig 36 Its aperture can be adjusted by rotating the aperture iris diaphragm lever When the U BI90CT binocular tube is used the aperture iris diaphragm can be viewed by setting the turret to position CT In general with dyed specimens optimum observation is possible by controlling the aperture iris diaphragm to between 70 and 80 of the aperture number of the objective W...

Page 30: ...hickness of filters does not exceed 11 mm Using appropriate filters according to the purposes allows you to observe and photograph specimens more effectively Particularly the use of the 45LBD filter is recommended in observation and photomicrography because it renders more neutral colors More than one filter with a diameter of 45 mm can be stacked in the filter holder Fig 38 Fig 39 4 Using the Fil...

Page 31: ...ld iris diaphragm image into focus 7 While gradually opening the field iris diaphragm rotate the condenser centering knobs ƒ on the condenser holder to adjust so that the field iris diaphragm image is centered in the field of view of the eyepieces 8 To check centration open the field iris diaphragm until its image inscribed the field of view Now the condenser is centered In actual observation open...

Page 32: ... into focus 7 Using the field iris diaphragm lever stop down the field iris diaphragm until its image is just inside the field of view 8 Slowly slide the condenser height fine adjustment knob toward the front to bring the field iris diaphragm image into focus When focusing is obtained rotate the knob clockwise to clamp it 9 While gradually opening the field iris diaphragm rotate the condenser hold...

Page 33: ...l When using the U BI90CT binocular observation tube the aperture iris diaphragm can be observed by setting the turret to position CT or using the U CT30 centering telescope 3 Flipping Up the Condenser Holder Fig 43 To create working space and facilitate specimen replacement micromanipulator positioning or mounting an objective from the side of the stage flip up the condenser holder upwards 1 Plac...

Page 34: ... cap Objective Series 3 Remark Oil proof cap Objective Series 3 Remark C1 UPlanFl100XO3 100XO3Ph 100XO3P Provided with microscope frame C1 1 piece C2 2 pieces C1 UApo20X3 340 40X3 340 40XOI3 340 20XW3 340 40XW3 340 UPlanApo10XW3 10XO3 60XW3 60XW3 IR 1 piece is provided with each objective C2 UPlanFl60XOI3 60XOI3Ph 100XO3 UPlanApo20XO3 40XOI3 40XOI3Ph 100XOI3 100XOI3Ph PlanApo60XO3 60XO3Ph 100XO3 C...

Page 35: ... objective 3 Remove the specimen and move the stage insert cut out close to the objective front lens Apply a drop of the provided immersion oil to the objective front lens Place the specimen and rotate the fine adjustment knob to bring the specimen into focus Use as little oil as possible Also remove the oil immediately after use If the oil contains air bubbles the image will be degraded Make sure...

Page 36: ... IX PH2 small Ph2 UPlanFl40XPh LCPlanFl40XPh LCPlanFl60XPh UPlanApo20XPh LCAch40XPh LUCPlanFl40XPh SLCPlanFl40XPh IX PH3 large Ph3 UPlanFl100XO3Ph UPlanApo40XOI3Ph UPlanApo100XOI3Ph PlanApo60XO3Ph Insert the optical element small in the 30 mm position and the optical element large in the 38 mm position For well observation it is recommended to use the IX PHC to obtain the phase contrast effect in ...

Page 37: ...Attaching the Phase Contrast Optical Elements With the IX ULWCD Condenser Note The IX PHCU or IX PH1U can be attached only in the Ph1 or PhC position Do not remove the built in elements With the U UCD8 Universal Condenser or IX2 MLWCD Condenser Refer to their separate instruction manuals Optical Element Indication Applicable Objectives PHL Built in PhL UPlanFl4XPh IX PHCU Ph1 CPlan10XPh LCAch20XPh...

Page 38: ...ed Do not tighten the optical element centering knobs too much for this may deform the frames of the optical elements 6 Place the index provided with each optical element in the index inser tion hole having the same number Š as the number of the position in which the corresponding optical element is mounted Figs 49 50 Note that the IX ULWCD does not have the index The optical element with the numb...

Page 39: ...arked so that the ring slit image overlaps with the phase plate of the objective A ghost of the ring slit image may be observed In this case overlap the brightest image with the phase plate If a thick specimen is moved the ring slit image may be deviated from the phase plate and the contrast may be deteriorated In this case re adjust the centering by repeating steps 1 to 5 above 6 After completing...

Page 40: ...t insert it in the idle position so that the surface with indication faces upwards 2 When attaching the IX2 SLPHC or IX2 SLPH1 optical element in the center position insert the provided centering knobs into the screw holes loosen them insert the optical element and tighten lightly the centering knobs again 1 Phase Contrast Optical Elements and Applicable Objectives Optical Element Applicable Objec...

Page 41: ...s used to focus on the ring slit and the phase plate of the objective Figs 56 57 5 Using the optical element centering knobs turn the two centering screws ƒ of the phase contrast slider so that the ring slit image overlaps with the phase plate of the objective A ghost of the ring slit image may be observed In this case overlap the brightest image with the phase plate If a thick specimen is moved t...

Page 42: ...ge in the 38 mm position 5 3 Differential Interference Contrast Observation Using the IX2 ILL100 Column DIC Slider Applicable Objective U DICT U DICTS Shift Type U DICTHC High Contrast Type U DICTHR High Resolution Type To be used in the BFP1 position of the DIC slider UIS2 Series UPlanSApo 10X2 IX2 DIC10 IX2 DIC10 20X IX2 DIC20 IX2 DIC20 IX2 DIC20HC IX2 DIC20HR 20XO 40X IX2 DIC40 IX2 DIC40 IX2 DI...

Page 43: ... IX DP40HC IX DP40HR 40XOI3 IX DPAO40 IX DPO40S 60X 100XOI3 IX DPO100 IX DPO100 PlanApo 60XO3 IX DPO60S UPlanApo 10XW3 IX DP10 IX DP10 60XW3 IX DPO60 IX DPO60 60XWPSF IX DPO60S IX DPO60S 60XW3 IR UApo 20X3 340 IX DPUA20 IX DPUA20 20XW3 340 40X3 340 IX DP40 IX DP40 IX DP40HC IX DP40HR 40XW3 340 IX DPAO40 IX DPO40S 40XOI3 340 DIC Slider Applicable Objective U DICT U DICTS Shift Type U DICTHC High Co...

Page 44: ...e index of the U ANT analyzer and the positioning groove of the U DICT DIC slider and drop the analyzer into the analyzer mount of the DIC slider Fig 60 3 Hold the U DICT so that the side with indication faces down insert it into the revolving nosepiece and tighten the clamping knob With the U DICTS U DICTHC U DICTHR DIC Slider The U ANT analyzer cannot be mounted on these sliders Use the IX2 AN a...

Page 45: ...4 When the U BI90CT binocular observation tube is used rotate the CT turret to position CT to engage the CT lens in the light path Fig 65 When the U BI90 U TBI90 binocular observation tube or U TR30H 2 trinocular observation tube is used remove an eyepiece and attach the U CT30 centering telescope 5 Rotate the focus ring or with the centering telescope rotate the knurled section to bring the objec...

Page 46: ...in sensitive color observation using the U UCD8 engage the U UCDTP530 1 plate sensitive color plate in the light path Setting the background color to dark enables an observation like darkfield observation Setting the background color to gray provides observation with high contrast and 3D feeling with the gray sensitive color with which the sensitivity is highest Setting the background color to gra...

Page 47: ...objective to be used to the revolving nosepiece and rotate it to engage the objective in the light path 4 Slide the polarizer rotation clamping knob on the polarizer unit horizontally in the counterclockwise direction around the axis until the position with which the field of view is darkest Fig 67 5 Clamp the polarizer rotation clamping knob so that the polarizer will not rotate 6 Place a specime...

Page 48: ... red index on the IX SPT straight photo tube then fit the circular dovetail of the straight photo tube into the photo port of the trinocular observation tube 3 Tighten the clamping screw firmly When the Side Port is Used In Combination with the IX2 SPT Fig 69 1 Loosen the side port clamping screw of the microscope frame using the Allen screwdriver and remove the cap 2 Align index on the IX2 SPT st...

Page 49: ...I90CT and U BI90 binocular observation tubes The finder eyepieces show four photo masks The numbers given to the masks correspond to the magnifications of the photo eyepiece Fig 72 1 Adjust the diopter of the eyepieces and focus on the photo masks in advance Focus so that the double crosslines are sharply visible as two distinct lines Fig 72 2 Bring the specimen into focus using the coarse fine ad...

Page 50: ... points Reduce light intensity for observation as low as possible Use additional heat barrier filters for observation For time lapse photography synchronize the on off of the light bulb with the exposure 8 Setting the Filter and Lamp Intensity Set the lamp intensity and filters in accordance with the film to be used Film Filter Light Intensity Control Button Daylight color film 45LBD IF Camera sym...

Page 51: ...struction manual for the TV adapter to be used 2 Attaching the Camera on the Trinocular Observation Tube H Fig 74 Compatibility with various mounts from different manufacturers can be obtained by attaching the IX SPT straight photo tube on the U TR30H 2 trinocular observation tube H and adding a TV adapter system for the BX series A C mount or F mount video camera can be attached by attaching the ...

Page 52: ...the Bottom TV Port IX2 TVR A C mount video camera used together with the U FMT or F mount video camera used together with the U FMT can be used by ordering the manufacturer to install and adjust the IX2 TVR TV port unit for bottom panel Even after modification the binocular 100 observation or TV 100 observation are possible by setting the light path selector lever 6 TV Observation 1 Adjusting the ...

Page 53: ...he revolving nose piece clicks properly into place Light path selector knob is set for the side port light path Set the knob to the binocular eyepiece light path position or increase the light intensity Too many filters are used Reduce the number of filters to the minimum required Stage center plate is engaged in the light path Move the stage and place the specimen again Field iris diaphragm is no...

Page 54: ...ge glares Condenser is raised too high Lower to the proper position Aperture iris diaphragm is stopped down too far Open the aperture iris diaphragm f Visibility of observed image is poor Image is not sharp Contrast is poor Details are poorly visible Objective in use is not designed for UIS2 UIS series Replace with an objective designed for UIS2 UIS optics Correction collar on the objective equipp...

Page 55: ...ginning but see the overall field of view It is sometimes recommended to turn your eyes away from the eyepieces look far off and look into the eyepieces again i The coarse fine adjustment knobs will not rotate easily or at all The transport lock is not released Release the transport lock The rotation tension adjustment ring is too tight Loosen the ring optimally The pre focusing lever is locked Un...

Page 56: ...ece possible Eye point switchable in 2 steps of 400 and 430 mm U TBI90 Binocular tube with 35 to 85 tilting angle Diopter adjustment on one eyepiece possible Eye point 406 to 471 mm U TR30H 2 Trinocular tube with 30 tilting angle Can be installed by means of the IX ATU intermediate tube Eye point 434 mm U MO Monocular tune with 45 tilting angle 10X eyepiece fixed Condensers IX2 LWUCDA2 IX2 LWUCD N...

Page 57: ...O NA 1 3 WD 0 2 mm with correction collar UPlanFLN60X NA 0 9 WD 0 2 mm UPlanFLN60XOI NA 1 25 0 65 WD 0 12 mm with iris diaphragm UPlanFLN100XO2 NA 1 3 WD 0 2 mm UPlanFLN100XOI2 NA 1 3 0 6 WD 0 2 mm with iris diaphragm Plan semi apochromat for DIC and fluorescence observation LUCPlanFLN20X NA 0 45 WD 6 6 7 8 mm with correction collar LUCPlanFLN40X NA 0 6 WD 2 7 4 0 mm with correction collar LUCPlan...

Page 58: ...pecifications UIS Series For phase contrast observation SLCPlanFl40XPh NA 0 55 WD 7 7 mm ring slit IX PH2 with correction collar For DIC and brightfield observation SLCPlanFl40X NA 0 55 WD 7 7 mm with correction collar For reflected fluorescence Fura 2 observation UApo20X3 340 NA 0 75 WD 0 55 mm with oil proof cap UApo40X3 340 NA 0 9 WD 0 2 mm with oil proof cap UApo40XOI3 340 NA 1 35 WD 0 1 mm wi...

Page 59: ...te instruction manuals Illumination column IX2 ILL100 High intensity light source U LH100HG U LH100HGAPO U LH75XEAPO Critical illumination lens IX CI Illumination column IX2 ILL30 Power supply unit TL4 TH4 connection cable IX71 only Frame clamping plates IX2 FP Hand switch TH4 HS Power supply unit TH4 Microscope frame IX71S1F 3 IX71S8F 3 IX51S1F 3 IX51S8F 3 Eyepieces WHN10X WHN10X H Trinocular obs...

Page 60: ...0 in the direction of the arrow 4 While pushing down the bulb clamping lever down hold the halogen bulb ƒ with gloves or a piece of gauze insert the bulb pins straight and fully into the pin sections on the lamp socket Then return the lamp clamping lever gently back to the original position to clamp the bulb 1 Mounting the Illumination Column Fig 76 Fig 76 Fig 77 Fig 78 Fig 79 ƒ To prevent reduced...

Page 61: ... Bulb Replacement During or Right After Use The bulb lamp socket and areas near these will be extremely hot during and right after use Set the main switch to OFF disconnect the power cord from the wall outlet then allow the old bulb and lamp socket to cool be fore replacing the bulb with a new of the designated type Attaching the Condenser Fig 81 1 Align the index groove on the condenser with the ...

Page 62: ...Screw the IX SCL stage clips into the threaded holes provided on the stage top surface Fig 85 The IX2 SFR stage can be mounted by reversing the front and rear to implement a stage with knobs on the right position In this case however the left fine adjustment knob comes in the way of the stage operation so it may be necessary to remove the left fine adjustment knob by removing the clamping screw us...

Page 63: ...aped notch on the positioning bracket Fig 89 2 Using the Allen screwdriver tighten the clamping screw of the bracket lightly to the stage Fig 89 3 Align the stage s center alignment index line with the edge ƒ of the upper stage Fig 90 4 Slide the positioning bracket to and fro in order to determine the most convenient stalk angle 5 Tighten the bracket s clamping screw firmly Mechanical Stage IX MV...

Page 64: ...ard and is more exposed to contamination than the objectives of upright microscopes Therefore if there are empty positions in the nosepiece attach the dust caps provided Fig 91 6 Figs 92 93 1 Loosen the condenser clamping screw 2 Fit the condenser into the mount dovetail on the condenser holder and push in the condenser until its positioning pin fits into the positioning groove on the mount doveta...

Page 65: ...it position Fig 95 2 Loosen the condenser clamping knob Fig 95 3 Insert the mount of the UCD adapter into the mount dovetail of the condenser holder the condenser turret should be positioned on the right hand side Push the UCD adapter horizontally into the condenser holder until the condenser s positioning pin fits into the positioning groove on the mount dovetail 4 Tighten the condenser clamping ...

Page 66: ... detached turned 180 and then attached on the observation tube mount again This lowers the eye point height by approximately 30 mm Fig 97 U TR30H 2 Tube Figs 98 99 First mount the IX ATU intermediate tube 1 Using the Allen screwdriver loosen the observation tube clamping screw on the observation tube mount Fig 98 2 Insert the circular dovetail mount of the intermediate tube into the observation mo...

Page 67: ...wn on the right in the figure Using the Accessory Mounting Holes Fig 101 Twelve threaded holes are provided on the microscope frame for mount ing accessories such as a micromanipulator These holes are covered with plastic dust caps To remove a cap insert the tip of a flat blade screwdriver sideways into the space between the cap and the frame The applicable screws are M6 screws The screws can be i...

Page 68: ... provided by Olympus If no power cord is provided please select the proper power cord by referring to the section PROPER SELECTION OF THE POWER SUPPLY CORD at the end of this instruction manual 3 Connect the power cord connector ƒ to connector firmly 4 Connect the power cord plug to a wall outlet Be sure to supply power from a grounded 3 conductor power outlet using the proper power cord If the po...

Page 69: ...se the rear plate but uses the stabilizing feet clamping holes 1 Tilt the microscope frame slightly and insert the four rubber foot seats into the rubber foot sections This will disable the cushions of the rubber feet This step is not required when you will not use the rubber feet 2 Using the Allen wrench temporarily attach the provided screws together with the spacers to the left and right plates...

Page 70: ...ent by referring to Specifications and Certified Cord below CAUTION In case you use a non approved power supply cord for Olympus products Olympus can no longer warrant the electrical safety of the equipment Specifications Voltage Rating Current Rating Temperature Rating Length Fittings Configuration 125V AC for 100 120V AC area or 250V AC for 220 240V AC area 6A minimum 60 C minimum 3 05 m maximum...

Page 71: ...s Service for Cables BASEC BASEC HAR 10 10 30 N V KEMA KEMA KEUR HAR 10 30 30 SEMKO AB Svenska Elektriska Materielkontrollanstalten SEMKO HAR 10 10 50 Österreichischer Verband für Elektrotechnik ÖVE ÖVE HAR 30 10 50 Danmarks Elektriske Materielkontrol DEMKO DEMKO HAR 30 10 30 National Standards Authority of Ireland NSAI NSAI HAR 30 30 50 Norges Elektriske Materiellkontroll NEMKO NEMKO HAR 10 10 70...

Page 72: ... time has exceeded 20 000 hours 2 Lamp does not light sometimes even though the main switch is set to on Except discharge burners 1 3 Illumination flickers when you move the lamp cable or lamp housing 4 Lamp cable is unusually hot to the touch 5 Scorching or burning odor is produced during use 6 Illumination still flickers after replacement with a new lamp bulb Except discharge burners 1 7 Deforma...

Page 73: ...MEMO ...

Page 74: ...MEMO ...

Page 75: ......

Page 76: ...any 3500 Corporate Parkway P O Box 610 Center Valley PA 18034 0610 U S A One Corporate Drive Orangeburg NY 10962 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 31 Gilby Road Mount Waverley VIC 3149 Australia 5301 Blue Lagoon Drive Suite 290 Miami FL 33126 U S A EC REP 01 10 ...

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