50
Problem
Cause
Remedy
Page
Magnification selector knob is in an
intermediate position.
Engage the knob all the way in or at a
click stop according to the observation
method.
Revolving nosepiece or magnification
selector knob is in an intermediate
position.
Engage the revolving nosepiece or
knob at a click stop.
Condenser turret is set in an intermediate
position.
Engage the condenser turret at a click
stop.
A filter is stopped in an intermediate
position.
Set the filter at the appropriate position.
The frost filter is not engaged.
Engage the frost filter.
d) Dirt or dust is visible in the field of
view.
Dirt/dust on the specimen.
Clean thoroughly.
Dirt/dust on the eyepieces.
Dirt/dust on a mirror unit.
Dirt/dust on the optical element.
Condenser is not correctly positioned
and the frosted filter or filter is focused.
Adjust the condenser height until the
field iris diaphragm image is formed
in the specimen plane.
e) Image glares.
Condenser is raised too high.
Lower to the proper position.
Aperture iris diaphragm is stopped down
too far.
Open the aperture iris diaphragm.
f ) Visibility of observed image is poor.
· Image is not sharp.
· Contrast is poor.
· Details are poorly visible.
Objective in use is not designed for UIS2/
UIS series.
Replace with an objective designed
for UIS2/UIS optics.
Correction collar on the objective
equipped with correction collar is not
adjusted.
While focusing, rotate the correction
collar to find the best position.
Front lens of the objective is dirty.
Clean the objective.
Immersion oil is not being used with an
oil immersion objective.
Use immersion oil.
Immersion oil contains bubbles.
Remove bubbles.
Inappropriate slide or cover glass
thickness.
Replace with glass of appropriate
thickness.
Dirt/dust on glass components (condenser,
objective, eyepieces, culture vessels,
etc.)
Clean thoroughly.
Ring slit and phase plate are not centered.
Center them correctly.
Poor contrast during DIC observation.
Replace the plastic culture vessel with
a glass vessel.
15
15
–
23/26
23/26
4
27/28
27/28
25/29
53,54
31
4
31
31
–
4
35/37
38/39
c) (Contd.)
