Nikon Eclipse Ni-U, Instruction Manual

Page: 35 / 52

Chapter 2
Troubleshooting
27
T
roub
le
shooti
ng
Problem Cause Measure
An aperture diaphragm is stopped down too far.
Otherwise, it is open too much.
Open it to the proper size.
( → See Chapter 3, “6 Adjusting the Aperture Diaphragm” in
the “Operation”.)
The field diaphragm image is not focused on the
specimen surface.
(Condenser adjustment is incorrect)
Make sure the condenser is focused and centered.
(See Chapter 3, “5 Focusing and Centering the Condenser”
in the “Operation”.)
No immersion oil is applied to the tip of an
oil-immersion objective.
The designated immersion oil is not used.
Apply our designated non-fluorescent immersion oil.
( → See Chapter 3 “12 Oil Immersion/Water Immersion” in
the “Operation”.)
The immersion oil contains air bubbles.
Remove the air bubbles.
( → See Chapter 3 “12 Oil Immersion/Water Immersion” in
the “Operation”.)
Image quality is poor.
Contrast is poor. Resolution
is poor.
The immersion oil adheres to the tip of the
dry-type objective.
Clean it as appropriate.
( → See Chapter 3 “12 Oil Immersion/Water Immersion” in
the “Operation”.)
The ND filters are out of the optical path.
Place the ND filters into the optical path.
( → See Chapter 3 “1.2 Adjustment with ND Filters” in the
“Operation”.)
Field of view is too bright.
The lamp voltage is too high.
Turn the brightness control knob to the mark
position and adjust the brightness with the ND
filters. (See Chapter 3, “1 Adjusting the Brightness
of a Diascopic Image” in the “Operation”.)
The lamp voltage is too low.
Turn the brightness control knob to the mark
position and adjust the brightness with the ND
filters. (See Chapter 3, “1 Adjusting the Brightness
of a Diascopic Image” in the “Operation”.)
The condenser aperture diaphragm is stopped
down too far.
This should normally be adjusted to 70 to 80% of
numerical aperture of the objective.
( → See Chapter 3, “6 Adjusting the Aperture Diaphragm” in
the “Operation”.)
The field diaphragm image is not focused on the
specimen surface.
Make sure the condenser is focused and centered.
( → See Chapter 3, “5 Focusing and Centering the
Condenser” in the “Operation”.)
Field of view is too dark.
The optical path is not switched to binocular
100%.
Set to binocular 100%.
( → See Chapter 3, Section 10 “Switching the Optical Path
of the Tube” in the “Operation”.)
The NCB11 filter is not used.
Use the NCB11 filter.
( → See Chapter 3 “1.2 Adjustment with ND Filters” in the
“Operation”.)
Image is yellowish or very
bluish.
Lamp voltage is too low or too high.
Turn the brightness control knob to the mark
position and adjust the brightness with ND filters.
(See Chapter 3, “1 Adjusting the Brightness of a Diascopic
Image” in the “Operation”.)
Visually observed image
color does not match color
of image on monitor.
White balance of the camera is not set correctly.
Set the white balance in accordance with the
camera's instruction manual.
The entire field of view is
bluish or yellowish.
A filter cube is in the optical path even though
epi-fluorescence observation is not being
performed.
Remove the filter cube from the optical path.