Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)
3.4 Epi-fluorescence Microscopy
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3.4
Epi-fluorescence Microscopy
Before microscopy...
•
Check the cumulative operating hours of the lamp in the Epi-illumination
attachment. Replace the lamp if its cumulative operating hours exceed the
recommended maximum service life.
•
Use non-fluorescent slide glass.
•
Use non-fluorescent immersion oil.
•
To keep specimen colors from fading, keep the shutter closed when not
performing microscopy.
1
Perform steps 1 through 11 in "3.3 Bright-Field
Microscopy."
2
Lower the condenser, or remove the condenser and mount
the shielding tube in its place.
3
Turn off the microscope power
switch.
4
Move the filter cube for the
excitation method to be used
into the optical path.
5
Fully open the field diaphragm
and aperture diaphragm of the
Epi-illumination.
Push to fully open
the field diaphragm.
Select a cube using the excitation
method switchover turret.
Press the power switch
to the "
◯
" position.
Push to fully open
the aperture
