Nikon ECLIPSE 80i Instructions Manual Download Page 45 | Manualshive

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Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)

3.3 Bright-Field Microscopy

43

3

9

Set a specimen and move the
portion to be viewed into the
optical path.

10

Focus on the specimen.

11

Adjust the diopter and the
interpupillary distance.

Refer to "8. Diopter Adjustment" and "9.
Interpupillary Distance Adjustment" in the separate
Reference manual.

Set a specimen and secure in

place using the specimen holder.

Move the portion to be viewed into the

optical path using the XY stage knobs.

Focus on the specimen using

the coarse and fine focus knobs.

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Summary of Contents for ECLIPSE 80i

Page 1: ...Microscope ECLIPSE 80i Instructions Microscopy M318E 03 12 CF 1 1 2 ...

Page 2: ......

Page 3: ...any other products attached to or to be used with this microscope super high pressure mercury lamp power supply high intensity light source etc Warning Caution symbols used in this manual Although Nikon products are designed to provide the utmost safety ignoring safety precautions or improper use may result in personal injury or property damage as well as voiding the terms of the warranty To ensur...

Page 4: ...biohazard risks To avoid biohazard contamination avoid touching the contaminated portion with bare hands Decontaminate the contaminated part according to the standard procedure specified for your laboratory Caution for heat This symbol found on the lamphouse of the ECLIPSE 80i indicates the following The lamp and surrounding areas including the lamphouse become very hot during and immediately afte...

Page 5: ...equires special care during handling Make sure you have read the instruction manual for the light source super high pressure mercury lamp power supply or high intensity source 4 Power cord for microscope and the power cord for AC adapter Use one of the power cords specified Using the wrong power cord may result in fire or other hazards The product is classified as subject to Class I protection aga...

Page 6: ...he lamp is scratched fouled subjected to high external pressure or physical impact or used beyond its service life the sealed gas may escape or the lamp may burst resulting in gas inhalation injury from glass or other injury 3 When the lamp is lit the lamp and surroundings will become extremely hot Touching the lamp with bare hands may result in burns flammable materials placed near the lamp may i...

Page 7: ...he lamp without first turning off the power switches for the microscope and the peripheral devices press them to the position and unplugging the power cord from the wall outlet Make sure the lamphouse cover is securely fitted to the lamphouse after lamp replacement Never turn on the lamp while the lamphouse cover is open Do not break up used lamps instead dispose of them as special industrial wast...

Page 8: ...pe Do not install the product in a locker or cabinet 7 Remove any covers from the product before switching on Do not use the product while covered with a cloth etc as this will give rise to abnormal heat which could cause a fire 8 Caution concerning long sustained observations To relieve fatigue resulting from long observation sessions limit continuous observations to one hour Take at least 10 to ...

Page 9: ...ons or poor performance Consider the following factors when selecting an installation location Select a vibration free location Install the product on a level surface Install the product at least 10 cm away from walls Choose a location less exposed to hazards in the event of collisions earthquakes or other potential disasters To keep the product from falling use strong rope or other means if neces...

Page 10: ...mperature changes When a filter is not in use store in a desiccator or hermetically sealed container with a drying agent The filters in the nine types of filter cubes listed below offer sharp high resolution waveform characteristics superior to normal filters However due to their sophisticated coatings they must be handled with special care In particular take care to avoid abrasion from cleaning F...

Page 11: ...e C TEP DSC Port for Ergonomic Binocular Tube DSC Port D FL Epi illumination Attachment Epi illumination Attachment D DH Digital Imaging Head M DIH M D CB C Box C Box D LH Precentered Lamphouse Lamphouse D NID6 Intelligent Sextuple DIC Nosepiece Intelligent Nosepiece C HS Hand Switch Hand Switch DS Camera Head DS 5M Camera Head DS Camera Control Unit DS L1 DS L1 DS Camera Cable Camera Cable Super ...

Page 12: ...t follow Please read this manual carefully before operating the product Manual 2 Reference describes the operations of each attachment Please read an appropriate section as necessary Title Importance Content Manual 1 Microscopy Must be read Safety precautions Microscopy Assembly Troubleshooting Cleaning and maintenance Specifications Manual 2 Reference As necessary Detailed operations of attachmen...

Page 13: ...ght field set Eyepiece tube mounted directly on 80i Camera and monitor are optional See Chapter 1 For operating procedures and detailed information on individual parts refer to the separate Reference manual 80i Epi illumination attachment Camera and monitor are optional See Chapter 2 For operating procedures and detailed information on individual parts refer to the Reference manual 80i DIH M Camer...

Page 14: ...oscopy 18 1 4 Photomicroscopy 22 Chapter 2 Part Names and Microscopy Procedures With Epi illumination Attachment Mounted 24 2 1 Names of Main Components 24 2 2 Names of Parts Used to Make Adjustments 25 2 2 1 Right view 25 2 2 2 Left view 26 2 2 3 Rear view without accessory device 26 2 3 Bright Field Microscopy 27 2 4 Epi fluorescence Microscopy 31 2 5 Photomicroscopy 35 Chapter 3 Part Names and ...

Page 15: ...ical 64 6 2 Electrical 65 Chapter 7 Cleaning and Maintenance 66 7 1 Lens cleaning 66 7 2 Cleaning the product 66 7 3 Disinfecting the product 66 7 4 Storage 67 7 5 Periodic Inspections fee charged 67 Chapter 8 Specifications 68 8 1 Specifications 68 ...

Page 16: ...ece tube Eyepiece Objective Specimen holder Condenser Stage Revolving nosepiece DSC port Required for image capture Camera head Required for image capture One example shown in the photo Lamphouse Camera control unit Required for image capture One example shown in the photo Microscope main body ...

Page 17: ...s 15 1 1 2 Names of Parts Used to Make Adjustments 1 2 1 Right view Diopter adjustment ring Optical path switching lever Coarse focus upper limit clamp Aperture diaphragm lever Field diaphragm knob Filter IN OUT switches Fine focus knob X stage knob Y stage knob Provided on the left side in certain products ...

Page 18: ...w 1 2 3 Rear view Coarse focus knob Fine focus knob Power ON indicator Preset switch Condenser focus knob Condenser centering screw Coarse focus torque adjustment knob Brightness control knob Tool storage compartment Power switch Lamphouse AC power inlet Input voltage indication Provided on the right side in certain products ...

Page 19: ...opy Procedures Bright Field Set 1 2 Names of Parts Used to Make Adjustments 17 1 1 2 4 Ergonomic binocular tube with camera attached Camera centering screws Camera fine focus adjustment screw C mount Camera head Camera cable connector ...

Page 20: ...eset switch 3 Move the ND8 ND32 and NCB11 filters into the optical path 4 Push in the optical path switching lever to direct the full optical path toward the binocular section Press the power switch to the position Press the preset switch The full optical path directed toward the binocular section Push in the optical path switching lever ND8 ND32 NCB11 ...

Page 21: ... path 7 Set the 10x objective into the optical path 8 Set a specimen and move the portion to be viewed into the optical path Set a specimen and secure in place using the specimen holder Move the portion to be viewed into the optical path using the XY stage knobs Fully open the aperture diaphragm using the aperture diaphragm lever Fully open the field diaphragm using the field diaphragm knob Raise ...

Page 22: ...illary Distance Adjustment in the separate Reference manual 11Focus and center the condenser Refer to 11 Adjustment of Condenser Position in the separate Reference manual Focus on the specimen using the coarse and fine focus knobs Center the condenser using the condenser centering screws Focus the condenser using the condenser focus knob Field diaphragm Eyepiece field of view Field diaphragm Eyepi...

Page 23: ...agm and aperture diaphragm each time you change objectives Field diaphragm Set slightly narrower than the margins of the field of view Aperture diaphragm 70 to 80 of the maximum numerical aperture N A of the objective 13Turn off power after completing observations Select the desired objective Press the power switch to the position Aperture diaphragm knob Field diaphragm knob ...

Page 24: ...rection After making the appropriate adjustments tighten the screws firmly 2 Focus adjustment If the image viewed through the eyepiece appears to be in focus but the image on the monitor is out of focus turn the camera fine focus adjustment screw on the C mount until the image on the monitor is in focus Use a low magnification lens to make this adjustment Note that such out of focus situations can...

Page 25: ...For fluorescent photomicrography adjust white balance under normal lighting conditions before shooting 6 Capture and save images Focus on the specimen Refocus Adjust image brightness using the camera exposure compensation function Check the image using the Freeze button If the image is acceptable press the CAPT button to save the image The operating procedure differs if DF FL scene mode is selecte...

Page 26: ...ination attachment uses a mercury xenon or high intensity halogen lamp as its light source It requires a separate power supply unit Before using the lamphouse be sure to read the lamp operating manual Camera head Required for image capture One example shown in the photo Lamphouse Camera control unit Required for image capture Microscope main body Photographic vertical tube adapter Required for ima...

Page 27: ...iaphragm knob Filter IN OUT switch X ステージハンド X stage knob Camera head Camera cable C mount adapter Excitation method switchover turret Excitation ID tag pocket Shutter open close lever Fine focus knob Filter cube mounting section inside Field diaphragm open close lever for Epi fluorescent light Aperture diaphragm open close lever for Epi fluorescent light Field diaphragm centering holes Aperture d...

Page 28: ...ocus knob Power ON indicator Preset switch Condenser focus knob Condenser centering screw Coarse focus torque adjustment knob Brightness control knob Tool storage compartment Power switch AC inlet Input voltage indication Lamphouse mounting section Lamphouse connector port ND4 filter slider ND16 filter slider ND8 filter slider Provided on the right side in certain products Left side the Epi illumi...

Page 29: ...er 2 Press the preset switch 3 Move the ND8 ND32 and NCB11 filters into the optical path 4 Push the optical path switching lever to direct the full optical path toward the binocular section Press the power switch to the position Press the preset switch ND8 ND32 NCB11 The full optical path directed toward binocular section Push in the optical path switching lever ...

Page 30: ...the uppermost position 7 Fully open the field diaphragm and aperture diaphragm When using the 1 100x condenser move the top lens into the optical path 8 Set the 10x objective into the optical path Fully open the aperture diaphragm using the aperture diaphragm lever Fully open the field diaphragm using the field diaphragm knob Select the 10x objective Raise the condenser using the condenser focus k...

Page 31: ...ustment in the separate Reference manual 12Focus and center the condenser Refer to 11 Adjustment of Condenser Position in the separate Reference manual Set a specimen and secure in place using the specimen holder Focus on the specimen using the coarse and fine focus knobs Center the condenser using the condenser centering screws Focus the condenser using the condenser focus knob Field diaphragm Ey...

Page 32: ...ield diaphragm and aperture diaphragm each time you change objectives Field diaphragm Set slightly narrower than the margins of the field of view Aperture diaphragm 70 to 80 of the maximum numerical aperture N A of the objective 14Turn off power after completing observations Select the desired objective Press the power switch to the position Aperture diaphragm knob Field diaphragm knob ...

Page 33: ...ide glass Use non fluorescent immersion oil To keep specimen colors from fading keep the shutter closed when not performing microscopy 1 Perform steps 1 through 11 in 2 3 Bright Field Microscopy 2 Lower the condenser or remove the condenser and mount the shielding tube in its place 3 Turn off the microscope power switch 4 Close the shutter and block the Epi illumination light 5 Move the filter cub...

Page 34: ...ation attachment then open the shutter and center the lamp Refer to the operating manual for the light source 8 Set the 10x objective into the optical path 9 Set a specimen and move the portion to be viewed into the optical path Fully open the field diaphragm Select the 10x objective Set a specimen and secure in place using the specimen holder Move the portion to be viewed into the optical path us...

Page 35: ...he fluorescence attachment to adjust brightness Adjust the field diaphragm so that it extends slightly beyond the field of view The image brightness can be adjusted with the aperture diaphragm Complete centering before using the aperture diaphragm When using oil immersion objectives apply immersion oil between the specimen and the objective Focus on the specimen using the coarse and fine focus kno...

Page 36: ...to move the position where no fluorescent cube is installed into the optical path If the condenser is not mounted install the condenser then focus and center the condenser Refer to step 12 of bright field microscopy Turn on the microscope power switch to turn on the diascopic light source 14Turn off all power switches after completing observations Press the power switch to the position Close the s...

Page 37: ...t the microscope for proper image observation See the directions given in previous 1 Bright Field Microscopy or 2 Epi fluorescence Microscopy 2 Adjust the camera head mounting position until the image is displayed properly 1 Adjustment based on stage motion directionLoosen the C mount adapter retaining screw and adjust the camera position so that moving the stage forward backward or left right mov...

Page 38: ...uitable for the microscopy method 5 Set the camera white balance To adjust white balance press the WB button while capturing an image of a clear section of a specimen slide For fluorescent photomicrography adjust white balance under normal lighting conditions before shooting 6 Capture and save images Focus on the specimen Refocus Adjust image brightness using the camera exposure compensation funct...

Page 39: ...uses a mercury xenon or high intensity halogen lamp as its light source It requires a separate power supply unit Before using the lamphouse be sure to read the lamp operating manual Camera head Required for image capture One example shown in the photo Lamphouse Camera control unit Required for image capture One example shown in the photo Microscope main body DIH M Shutter open close hand switch fo...

Page 40: ...r IN OUT switch X stage knob Y stage knob Camera head Camera cable C mount adapter C mount adapter retaining screw Excitation method switchover turret Excitation ID tag pocket Fine focus knob Filter cube mounting section inside Optical path switching lever 1 Zoom knob X ステージハンド Field diaphragm open close lever for Epi fluorescent light Aperture diaphragm open close lever for Epi fluorescent light ...

Page 41: ...3 2 2 Left view ND4 filter slider ND16 filter slider ND8 filter slider DIH M Power ON indicator Coarse focus knob Fine focus knob Power ON indicator Preset switch Condenser focus knob Condenser centering screw Coarse focus torque adjustment knob Brightness control knob Provided on the right side in certain products ...

Page 42: ...ry device Tool storage compartment Power switch AC inle Input voltage indication Lamphouse mounting section Lamphouse connector port C Box power switch Not used Connector for Epi illumination shutter open close hand switch AC adapter inlet Outside the C Box Inside the C Box Intelligent nosepiece connector DIH M connector C Box mounting sections ...

Page 43: ... the power switch is turned on Do not touch the hand switch If the shutter is opened accidentally press the hand switch once again to close the shutter 2 Press the preset switch 3 Move the ND8 ND32 and NCB11 filters into the optical path 4 Pull out both optical path switching levers and direct the full optical path toward the binocular section Press the power switch to the position Press the prese...

Page 44: ...d 6 Raise the condenser to the uppermost position 7 Fully open the field diaphragm and aperture diaphragm 8 Set the 10x objective into the optical path Fully open the aperture diaphragm using the aperture diaphragm lever Fully open the field diaphragm using the field diaphragm knob Raise the condenser using the condenser focus knob Select the 10x objective Rotate the excitation method switchover t...

Page 45: ...Focus on the specimen 11Adjust the diopter and the interpupillary distance Refer to 8 Diopter Adjustment and 9 Interpupillary Distance Adjustment in the separate Reference manual Set a specimen and secure in place using the specimen holder Move the portion to be viewed into the optical path using the XY stage knobs Focus on the specimen using the coarse and fine focus knobs ...

Page 46: ...ou change objectives Field diaphragm Set slightly narrower than the margins of the field of view Aperture diaphragm 70 to 80 of the maximum numerical aperture N A of the objective 14Turn off power after completing observations Select the desired objective Press the power switch to the position Center the condenser using the condenser centering screws Focus the condenser using the condenser focus k...

Page 47: ...ep specimen colors from fading keep the shutter closed when not performing microscopy 1 Perform steps 1 through 11 in 3 3 Bright Field Microscopy 2 Lower the condenser or remove the condenser and mount the shielding tube in its place 3 Turn off the microscope power switch 4 Move the filter cube for the excitation method to be used into the optical path 5 Fully open the field diaphragm and aperture...

Page 48: ...ess the hand switch to open the shutter of the Epi illumination and center the lamp Refer to the lamp operating manual 8 Set the 10x objective into the optical path 9 Set a specimen and move the portion to be viewed into the optical path Select the 10x objective Set a specimen and secure in place using the specimen holder Move the portion to be viewed into the optical path using the XY stage knobs...

Page 49: ...ence attachment to adjust brightness Adjust the field diaphragm so that it extends slightly beyond the field of view The image brightness can be adjusted with the aperture diaphragm Complete centering before using the aperture diaphragm When using oil immersion objectives apply immersion oil between the specimen and the objective Focus on the specimen using the coarse and fine focus knobs Field di...

Page 50: ...tion where no filter cube is installed into the optical path If the condenser is not mounted install the condenser then focus and center the condenser Refer to step 12 of bright field microscopy Turn on the microscope power switch to turn on the diascopic light source 14Turn off all power switches after completing observations Press the power switch to the position Use the excitation method switch...

Page 51: ...g position until the image is displayed properly 1 Adjustment based on stage motion direction Loosen the C mount adapter retaining screw and adjust the camera position so that moving the stage forward backward or left right moves the image on the monitor in the same direction After making the appropriate adjustments tighten the screw firmly 3 Make camera settings For a detailed discussion of this ...

Page 52: ...specimen slide For fluorescent photomicrography adjust white balance under normal lighting conditions before shooting 6 Capture and save images Adjust image brightness using the camera exposure compensation function Check the image using the Freeze button If the image is acceptable press the CAPT button to save the image The operating procedure differs if DF FL scene mode is selected For a detaile...

Page 53: ...dvice 2 Installing a Lamp 1 Loosen the lamphouse cover fixing screw and lift the lamphouse cover to remove 2 Attach the lamp to the lamp plug Avoid touching the bare lamp bulb with your bare hands Use the lamp type specified Using an unspecified lamp may cause malfunction or fire Refer to Chapter 8 for the specified lamp 3 Replace the cover and tighten the screw The lamphouse cover must be attache...

Page 54: ...of the microscope and fix it with the fixing screw 2 Attach the connector 4 Installing a Stage 1 Turn the coarse focus knob to remove the cushioning material from the elevating section Attaching the lamphouse Attaching the connector Turn this to lower the elevating stage Fixing screw ...

Page 55: ...evating section by aligning the projection on the back of the stage with the groove of the elevating section 3 Place a specimen holder on the stage and secure with screws Fixing the stage Fixing the stage with centering function Fixing the specimen holder Groove Stage with centering function Projection Back of the stage with centering function Screws fixing the stage ...

Page 56: ...the back of the microscope 4 Turn the condenser focus knob until the elevating section is raised to the uppermost position 6 Installing an Eyepiece Tube directly 1 Place an eyepiece tube on the microscope arm 2 Attach the eyepiece by aligning the projection of the eyepiece tube sleeve with the notch at the objective 3 Attach a screw hole protection sticker provided with the system to the bolt hole...

Page 57: ...ssure mercury lamp power supply 1 Attach a collector lens to the lamphouse 2 Turn the bayonet mount clockwise viewed from the back as far as it will go 3 Insert the lamphouse into position 4 Turn the bayonet mount anticlockwise viewed from the back to lock the lamphouse 5 Attach a mercury lamp 6 Connect the mercury lamphouse to the mercury lamp power supply 5 Attach the eyepiece tube 6 Attach the ...

Page 58: ...errite core side of the control cable to the CTL connector When inspecting DIH M conditions also attach the USB cable In this case the other end of the USB cable is connected to the PC The control cable and the USB cable are provided with the DHI M 6 Replace the cover C BOX fixing Viewed from the other side Cover fixing screw DIH M fixing screw Three positions Control cable USB cable Always connec...

Page 59: ...fixing screws attached then tighten the mount screw 5 Replace the cover 6 Loosen the zooming section fixing screw to remove the clamp from the DIH M 7 Attach the blind cap provided 8 Connect the control cable attached to the DIH M to the C BOX 9 Attach the hand switch for the Epi illumination shutter to the C BOX Attach the hand switch for the Epi illumination shutter here Connect the control cabl...

Page 60: ...et mount clockwise viewed from the back as far as it will go 3 Insert the lamphouse into position 4 Turn the bayonet mount anticlockwise viewed from the back to lock the lamphouse 5 Attach a mercury lamp 6 Connect the mercury lamphouse to the mercury lamp power supply 11 Attach the eyepiece by aligning the projection of the eyepiece tube sleeve with the notch at the objective Projection Notch Bayo...

Page 61: ... screw 3 When using the intelligent revolving nosepiece connect the connector attached to the revolving nosepiece to the upper port found inside the C BOX 10Installing Objectives Screw objectives into the revolving nosepiece When installing the objective in this way make sure that the magnification of the objective increases when the revolving nosepiece is turned clockwise clockwise when viewed fr...

Page 62: ...s as the one indicated on the filer cube select knob on the right side of the microscope 4 Turn the filter cube select knob and insert a filter cube into the remaining open position 5 Replace the cover 6 Attach a light shield to the front bottom of the fluorescent unit with screws Filter cube fitting part Excitation switching turret Address indication Address indication Address indication Nameplat...

Page 63: ...When attaching to the trinocular eyepiece tube 1 Attach the camera head to the trinocular eyepiece tube using the C mount adapter 2 Attach the camera cable to the camera head Adjust the fitted position before using the camera 12 3 When attaching the DIH M 1 Attach the camera head to the DIH M using the C mount adapter that is near the rear port 2 Attach the camera cable to the camera head Adjust t...

Page 64: ...ing the AC Adapter when using the DIH M 1 Confirm that the power switch of the C BOX attached to the back of the microscope is off the button projects out 2 Connect the AC adapter to the bottom port on the front of the C BOX 3 Connect the AC adapter to the wall outlet with the power cord Use only the AC adapter and power cord specified Refer to Chapter 8 for the specified AC adapter and power cord...

Page 65: ...t reduce lamp service life Avoid touching the bare lamp bulb with bare hands Soiling will reduce the service life of the lamp 1 Loosen the screw and lift the cover to remove 2 Remove the old lamp 3 Replace with a new lamp Avoid touching the bare lamp bulb with bare hands Use only the lamp specified Refer to chapter 8 for the specified lamp 4 Replace the cover and fix it with the screw The lamphous...

Page 66: ...nd dust on lens condenser eyepiece lens filter and specimen Clean Field diaphragm image not focused on the specimen surface Focus and center the condenser Dirt or dust in the field of view Field diaphragm closed too far Open the field diaphragm to the proper setting Field diaphragm closed or opened too far Open or close the field diaphragm to the proper setting Objective correction ring not adjust...

Page 67: ...switching lever to provide the target location with 100 light Specimen upside down Attach the specimen to the stage with the cover glass face up Unspecified cover glass thickness Attach a cover glass of the specified thickness 0 17 mm Focus impossible on a high magnification objective Diopter not adjusted Adjust the diopter Too great a difference in focus after objective switching Diopter not adju...

Page 68: ...ee or four times should be sufficient to clean the lens Never use petroleum benzine to clean the entrance lens at the bottom of the eyepiece tube or prism surface of the eyepiece tube Absolute alcohol and petroleum benzine are highly flammable Be careful when handling these materials particularly around open flames or when turning the power switch on or off Follow the instructions provided by the ...

Page 69: ...inyl cover over the microscope to protect it from dust Switch off the microscope press the switch to the O position and wait for the lamphouse to cool before covering the microscope with the vinyl cover 7 5 Periodic inspections fee charged To maintain the peak performance of the microscope we recommend periodic inspections Contact your nearest Nikon representative for more information Parts and se...

Page 70: ...ic binocular tube binocular section 100 binocular DSC port 50 50 DSC port mountable to ergonomic binocular tube Trinocular eyepiece tube FUW binocular straight 100 0 0 100 Trinocular eyepiece tube TUW binocular straight 100 0 20 80 0 100 Nosepiece section Sextuple nosepiece sextuple DIC nosepiece intelligent septuple nosepiece intelligent septuple DIC nosepiece Stage section Vertical stage vertica...

Page 71: ...C Box UL Listed Product UL61010A 1 Meets FCC 15B Class A requirements This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radi...

Page 72: ... Aperture stop Manual unit detachable Field stop Manual unit detachable ND filter Manual 3 filters slider type filters ND4 ND8 ND16 Shutter Motorized controlled with external hand switch Analyzer slot Yes side surface Polarizer slot Yes side surface Compatible lamp housing Hg Xe centered halogen incompatible with precentered type External connection USB C1 interlock D CB C BOX COM for maintenance ...

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