Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)
2.4 Epi-fluorescence Microscopy
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6
Fully open the field diaphragm
and aperture diaphragm of the
Epi-illumination attachment.
7
Switch on power for the light
source of the Epi-illumination
attachment, then open the
shutter and center the lamp.
(Refer to the operating manual
for the light source.)
8
Set the 10x objective into the
optical path.
9
Set a specimen and move the
portion to be viewed into the
optical path.
Fully open the field
diaphragm.
Select the 10x objective.
Set a specimen and secure in place
using the specimen holder.
Move the portion to be viewed into the
optical path using the XY stage knobs.
Open the shutter.
Fully open the
aperture diaphragm.
