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NanoEnTek JuLI FL, User Manual

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Fluorescence Cell History Recorder

User 

Manual

FL

NanoEnTek Inc. (HQ)

12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 08389, Korea

Tel. 

+82-2-6220-7940

Fax. 

+82-2-6220-7721

NanoEnTek USA Inc. 

5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA
Tel : +1-925-225-0108, +1-888-988-0108(Toll free)
Fax : +1-925-225-0109

Email

[email protected]  

Website

www.nanoentek.com 

NESMU-JUF-001E (V.1.0)

FL

Summary of Contents for JuLI FL

Page 1: ...26 gil Guro gu Seoul 08389 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 NanoEnTek USA Inc 5627 Stoneridge Drive Suite 304 Pleasanton CA 94588 USA Tel 1 925 225 0108 1 888 988 0108 Toll free Fax 1 925 2...

Page 2: ...e software firmware Check the disk space PC software Introduction Installation Function guide Home Open data Dual project creation Export data Confluence Edit movie file Recount bright data Recount fl...

Page 3: ...utor or e mail sales nanoentek com Model JULI FGSC or JULI FRSC Scope unit are only used in conjunction with the JULI BFST Station unit Scope unit JULI FGSC or JULI FRSC 1 EA USB drive 4 GB 1 EA Stati...

Page 4: ...ll culture incubator easily It can be used to compare the control and the experimental samples using the dual systems optional concurrently Features of JuLI FL Fluorescence live cell movie analyzer Ve...

Page 5: ...pturing time lapse imaging IMPORTANT JuLI FL is optimized into standard size of cell culture incubator It is recommend that the temperature should be stabilized in case of a small size incubator befor...

Page 6: ...monitoring the 2nd scope unit also should be connected to the station unit 4 Plug the power cord into the electrical outlet Be sure to only use the power cord supplied with the instrument Powering the...

Page 7: ...uality to obtain better cell images Stage place sample here LED JULI FLG04 Green Bright JULI FLR04 Red Bright Station unit LCD touch screen Contains buttons for necessary functions and displays the im...

Page 8: ...the Power button USB Port Connect a USB drive to save and transport images to PC Display port CRT port connect to external monitor resolution 1366 X 768 pixels make sure to turn off an instrument befo...

Page 9: ...wer cord and the appropriate plug based on the electrical outlet configuration in your country On off switch is main power switch It is not necessary to use the on off switch for day to day operation...

Page 10: ...toring menu Description User interface Focusing menu User interface Monitoring menu Zoom in Navigation Zoom out Focus interface Exposure D phase Auto focus Brightness Save Scale bar Capture Confluence...

Page 11: ...it channel Sensitivity Background level Dilution factor Image format S W version F W version OS version Disk space Date Time information LED power Monitoring data information Zoom bar Screen shot Dele...

Page 12: ...al changes between large or small sizes Measures cell measurement ranging from 1 10 4 to 1 10 7 cells mL optimal measurement range from 1 10 5 to 4 10 6 cells mL and cells with sizes ranging from 5 m...

Page 13: ...rescence mode 4 To adjust illumination intensity use the Exposure and Brightness bar on the touch screen Touch the arrow button for more minute adjustment 5 If necessary use the Zoom in button to magn...

Page 14: ...y to get the sample into coarse focus Fine focus is a smaller knob which moves short distances slowly and is used to get the sample in sharp focus The images below show the reference images to adjust...

Page 15: ...ual variations The Auto focus is the auto focusing function which automatically senses the focus optimized for the analysis software of the JuLI FL Operation Focusing Auto focus x1 IMPORTANT It is str...

Page 16: ...which automatically senses the focus optimized for the analysis software of the JuLI FL Operation Focusing ROI Auto focus x1 IMPORTANT It is strongly recommended to use ROI Auto focus function after...

Page 17: ...e value of confluence should be more accurate try again after adjusting parameters in the Setting menu Refer to page 40 The parameter is the recommended values for corresponding to cell line The defau...

Page 18: ...The images below show the guidelines for the focus to obtain an accurate cell confluence Bright mode only Du 145 Parameter A Good setting Exposure 4 Brightness 1 Normal setting Exposure 6 Brightness...

Page 19: ...s the Preview button to recapture if necessary 3 If necessary press the Save button to save the captured image Type the file name in the blank 4 Check the confirmation message Capture complete Press t...

Page 20: ...ssary 3 If necessary press the Save button to save the captured image Type the file name in the blank 4 Check confirmation message Capture complete Press the OK button 5 The saved data can be checked...

Page 21: ...ow the merged color image 3 If necessary press the Save button to save the merged image Type the file name in the blank 4 Check the confirmation message Capture complete Press the OK button 5 The save...

Page 22: ...d to station unit 2 Adjust the focus of JuLI FL and select the region that should be monitored Refer to page 14 15 Press the Setting button 3 Type the file name in the blank and the press the Save but...

Page 23: ...l The number of total images will be displayed automatically when data value of Total Time and Interval are set up IMPORTANT Warm up scope unit for 1 hour in incubator before making a movie If not it...

Page 24: ...ng data in Data menu Refer to page 32 7 Press the Rec button to begin making a time lapse movie JuLI FL displays live cell images continuously during recording If necessary to adjust the focus during...

Page 25: ...age as below will be shown Press the OK button Unexpected problems such that the power is off suddenly or the scope unit is not connected to station unit could be happened during making a movie Then t...

Page 26: ...are saved in default folder Captured Images 2 Each channel folder has subfolders ex Bright Fluorescence and Merge The control sample and experimental sample data can be found in the subfolders Data ca...

Page 27: ...er name in Data list or pressing the Enter button 4 The captured image can be shown by tapping its file name The images can be checked by pressing the Play button 5 Magnify movie screen or image scree...

Page 28: ...4 Select the screen size of snapshot using the Size icon Snapshot capturing process can be canceled by pressing the Cancel icon 5 After that double tap to save the selected region 6 Type the name in t...

Page 29: ...or files can be selected at once using the Multi select button If the wrong file is selected press the Multi select button or select the wrong file name you selected again 4 Press the Save to USB but...

Page 30: ...n Only main folder can be renamed not sub folders individually in case of dual monitoring data 3 Type new name of folder or file in the blank Then press the Save button 4 Check the confirmation messag...

Page 31: ...m using the Multi select button 2 Press the Delete button to delete selected folders or files If Captured Images folder is selected all image files in the folder will be deleted Only master folder can...

Page 32: ...4 Check the confirmation message Delete complete Press the OK button Operation Data Delete JuLITM FL Fluorescence Cell History Recorder 2013 NanoEnTek Inc 33...

Page 33: ...t Fluorscence mode 1 Load 10 L of sample mixture on the counting slide side A using a pipette The two chambers of the slide are labeled as A and B for easy tracking of your samples Each chamber is cou...

Page 34: ...nd rapid counting results The example images of HeLa cells show the reference value for adjusting focus Counting Auto focus Operation IMPORTANT It is strongly recommended to use Auto focus function af...

Page 35: ...utton are activated in the Bright mode If necessary set the dilution factor corresponding to the experiment condition Refer to page 44 3 The counting results including the total cell live cell dead ce...

Page 36: ...justing the number up increases inclusiveness thereby decreasing the lower cell size range Maximum cell size is used to determine the high range of cell size within the measurement The algorithm first...

Page 37: ...curate focus without individual variation 2 Press the FL Counting button for the cell counting The Br counting button and the FL counting button are activated in the Bright mode If necessary set the d...

Page 38: ...uorescence tap Operation Counting Fluorescence counting Bright counting result tap Graph Gating background Dead cell red bar Live cell blue bar Cell size gating bar Counting results Fluorescence count...

Page 39: ...nting result will be shown with the cell size distribution graph If you are not satisfied with the result then try again after adjusting the minimum the maximum cell size and the fluorescence intensit...

Page 40: ...g result file for one folder automatically In a folder there are one original cell image one cell counting image marked with different color for the viable and dead cells one screen shot image with ce...

Page 41: ...he background The increased sensitivity provides better recognition of fainter objects in the Bright mode Background level The decreased background level provides better recognition of fainter objects...

Page 42: ...p Down button Press the Set button if necessary to change the dilution factor when using the counting function The Default value is 2 for the bright counting using the trypan blue staining method and...

Page 43: ...o the experiment condition and the Apply button The JuLI FL supports various image formats including JPEG BMP TIFF and PNG Select one as mostly utilized format The default format is JPEG Setting Set u...

Page 44: ...updated software which will be supplied as a zip file to your desktop 3 Unzip the JuLI FL Update zip file and save the software in a USB drive 4 Connect the JuLI FL USB drive provided with JuLI FL to...

Page 45: ...Check the disk space of JuLI FL If no disk space left the saved data should be deleted in Data menu Refer to page 32 Operation Setting Check the disk space 46...

Page 46: ...f the operation system of your PC is upper version of Windows or Vista To install PC software follow the directions as below Check to ensure that your PC is connected to Internet before installing the...

Page 47: ...to proceed with the installation 4 If you want to change installation folder press the Browse button and choose the location that you want 6 The PC activates the Installation of the Software 7 If the...

Page 48: ...face as below image will be shown Home Zoom bar Image information Save with scale bar Insert scale bar Graph Data list Confluence Movie Open Save as Export Dual project creation Preview window Countin...

Page 49: ...Operation PC software Home 1 Press the Open button Folders and files can be opened by dragging and dropping on data list window directly 2 Browse the folder and select it PC software Open data 50...

Page 50: ...4 Select the name of image in the data list The preview window will show the image and movie you selected In the case of dual monitoring data control sample folder and experimental sample folder can b...

Page 51: ...using the Zoom bar 6 Select the viewing region by dragging the preview window 7 Press the Insert scale bar button to activate the scale bar on the preview window if necessary Press the Save with scal...

Page 52: ...e Dual project creation button in the Home menu 2 Select 2 different single monitoring data folder Selected movie files should have been monitored under the same interval and total time 3 Press the Ma...

Page 53: ...e saved Type the file name for saving exported file The file will be saved as csv file which is compatible with Excel and Notepad programs 3 Select the check box of each channels in the case of dual m...

Page 54: ...tivity and background level to the rest of images in the same folder Check the channels Channel 1 or Channel 2 and press the OK button to get new confluence value 5 Confluence graph would be redrawn 6...

Page 55: ...start point and end point by moving the bar 3 Check the Apply box to edit the movie 4 Press the Save as button to save the edited movie 5 Select the folder in which the edited movie should be saved a...

Page 56: ...on factor is set as 2 in this sample data 3 Select a File name of the bright image in the data list and press the Counting button to recalculate 4 The counting results including the total cell live ce...

Page 57: ...value for the minimum cell size and the maximum cell size touching the control bar to adjust minutely The set value will be immediately reflected in the Counting results Operation PC software Recount...

Page 58: ...o page 44 The dilution factor is set as 1 in this sample data 3 Press the Multi selection for FL counting button and select a pair of data including one bright image and one fluorescence image 4 Press...

Page 59: ...ing results including the total cell fluorescence cell non fluorescence cell and average cell size show up on the Fluorescence tap 7 The counting results including the total cell fluorescence cell non...

Page 60: ...nd average cell size show up on the Fluorescence tap The set value will be immediately reflected in the Counting results After fluorescence gating Before fluorescence gating Operation PC software Reco...

Page 61: ...hown 3 Select the modes Br or FL of each channel The JuLI FL supports the dual monitoring function that enables a user to compare the GFP RFP samples and Bright samples at the same time 4 Press the Cr...

Page 62: ...e of the merged movie and press the Save button 6 The merged movie file will be created after merging process is done Operation PC software Dual view JuLITM FL Fluorescence Cell History Recorder 2013...

Page 63: ...regular maintenance To troubleshoot problems with JuLI FL contact Technical Support Refer to page 72 IMPORTANT Never disassemble or service JuLI FL by yourself Unauthorized repairs may damage JuLI FL...

Page 64: ...instrument for 1 hour before monitoring The power should be on when warming up the instrument Cell culture flask should be wetted by the culture media before making a movie Eliminate any dust on the c...

Page 65: ...d as the last state before recording stopped Create movie file with time lapse images using JuLI FL PC software Download JuLI FL Update folder on NanoEnTek website www nanoentek com Check the files as...

Page 66: ...lied by anyone other than Nanoentek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample For your protection JuLI FL units being returned...

Page 67: ...strument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the wall outlet and...

Page 68: ...a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Disposal of your old appliance 1 When this crossed ou...

Page 69: ...I FLR04 Excitation 525 50 nm Emission 580LP Scope unit 300 x 190 x 188 mm 4 5 kg Station unit 282 x 285 x 160 mm 3 2 kg 320 GB Hard disk drive 4 GB USB drive AC100 240Va c 1 9A 100w 50 60 Hz 5 40 C 20...

Page 70: ...I FGSC JULI FLR04 JULI FLRD04 JULI FRSC Accessories JULI BRTB Disposable JULI BRS50 JULI BRS1000 JULI BRS5000 Description JuLI FL Single set JuLI FL Dual set Scope unit of JuLI FL JuLI FL Single set J...

Page 71: ...d special offers For more information or technical assistance please call or email NanoEnTek Inc HQ 12F 5 Digital ro 26 gil Guro gu Seoul 08389 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 NanoEnTek US...

Page 72: ...nTek Inc HQ 12F 5 Digital ro 26 gil Guro gu Seoul 08389 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 The information in this manual is described as accurately as possible The changes involved in firmwa...

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