00110230w Rev 10/20
18 of 24
Storage and Maintenance
Storage
• Store the Scepter™ 3.0 Cell Counter and Scepter™ 3.0 Sensors in a clean, dry environment at 15-30 °C.
• Do not expose the instrument, sensors or charger to UV light.
• Store Scepter™ 3.0 Test Beads in refrigerator after opening. Do not freeze.
Cleaning/Sanitizing
• The Scepter™ 3.0 is
NOT
autoclavable. Extreme heat will damage the display screen and other electronic
components.
• Keep the Service Test and USB port covers closed so no liquid enters any part of the instrument during cleaning.
• The Scepter™ 3.0 body and instrument control buttons can be sanitized by wiping with a soft cloth moistened
with 70% ethanol, ≤10% bleach solution, phenol-based solution (Sporicidin
®
Disinfectant), pre-saturated wipes
with bleach or alkyl with detergents (Hype-Wipe
®
disinfecting towels with bleach, Lysol
®
and Clorox
®
wipes). Do
not clean the display screen with sanitizing agents or other aggressive solutions. Wipe the screen with a soft,
dry, non-abrasive cloth.
CAUTION:
When sanitizing, make sure that no liquid enters any part of the instrument.
• The Scepter™ 3.0 Sensors are
NOT
reuseable.
Maintenance
Instrument repairs must be carried out by authorized personnel only. Contact tech service
at
.
Troubleshooting
Symptom
General Cause
Corrective Action
Questionable
concentration
Sensor not fully immersed in
solution while sample is loading
Keep sensor fully immersed while screen displays
Keep Sensor Submerged
.
Concentration of cell sample
is too high or too low
Make sure concentration of cell sample is within recommended
operating range. See
Cell, Bead, and Particle Suspensions .
Test sample using Scepter™ 3.0 Test Beads (Cat. No. PHCC3BEADS)
to ensure unit operating properly for concentration.
Wrong diluting solution
Use a diluting solution that is compatible with cells
being counted.
Cell clumping
Ensure that cells are in a single-cell suspension. Break
clumps by pipetting up and down with a standard pipette
or Pre-filter using Steriflip
®
filter unit with 20 µm nylon net.
Questionable
cell diameter
Diameter of cell sample
is too high or too low
Perform
procedure using the
Scepter™ 3.0 Test Beads to ensure it is operating properly for size.
Review histogram, Y-axis scale, and or reset gates manually
if possible. Refer to
for instructions on
adjusting the gates manually.
Wrong diluting solution
Use a diluting solution that is compatible with cells being
counted. See
Cell, Bead, and Particle Suspensions, p .12 .
Cell clumping
Ensure that cells are in a single-cell suspension. Break clumps
by pipetting up and down with a standard pipettor or pre-filter
using Steriflip
®
filter unit with 20 µm nylon net.
Failure to advance to
next display screen
Sensor is not inserted
correctly
Make sure the sensor size number and laminated circuit
are facing the front of the instrument.
Peak of interest indistinct
Y-Axis not optimized for peak of
interest
for instructions on
adjusting the Y-Axis.
Cell clumping
Sample has high level of debris or death.
Incorrect diluting solution.
Peak of interest not
selected by gates
Use of auto-gating feature
After counting, reset gates manually. Refer to
