Millipore Sigma PHCC30000 User Manual Download Page 3

00110230w  Rev 10/20 

3 of 24

Symbols and Safety Precautions

This system is intended for research use. Go to 

SigmaAldrich.com/techservice

 for assistance. 

IT IS IMPORTANT TO READ THESE INSTRUCTIONS BEFORE USING THIS INSTRUMENT .

Y

•  Please read these safety instructions and user manual carefully before using the cell counter and/or any accessory 

supplied with this product.

•  Keep these instructions for future reference.
•  If there is any contradiction between the information in the manual and the information in this notice, the information in 

this notice takes precedence.

•  The protection provided by this equipment may be compromised if it is used incorrectly.
•  Obey the instructions that are given to you. Do not perform any work on the inside of the instrument.

Y

This symbol indicates that there is a component inside the cell counter that might represent a particular hazard  

(refer to the operating manual) (HAZARD).

For Global Registration Marks of Conformity, p.24.

C

Affixed in accordance with the most recent European Council Directives which apply to this instrument.  

Please refer to the Declaration of Conformity for further detail.

Federal Communications Commission (FCC) conformity marking TFB-1003.

This symbol represents the products compliance to Chinese RoHS. For more information on RoHS compliance  

please see the products Declaration Of Conformity.

This product has been evaluated by an OSHA certified Nationally Recognized Testing laboratory (NRTL).

WARNINGS

•  To avoid danger of electric shock, do not install the instrument in an area with a high humidity level, such as a greenhouse or an incubator.  

Refer to 

Operating Environmental Conditions, p .22

•  Do not touch the charger station and plug with wet hands.
•  To avoid potential shock hazard, choose the correct plug configuration and make sure that the USB cable/power adapter  

is plugged securely into a properly grounded AC power outlet.

•  Always ensure that the power supply input voltage matches the voltage available in your location.
•  Do not use with flammable or explosive liquids.
•  Do not immerse instrument body in liquid, or allow liquid to enter any part of the instrument.
•  Do not expose instrument to vibrations. Vibrations may cause instrument malfunction or damage.
•  Do not autoclave or expose to high temperature.
•  To avoid damage to internal electronic components, do not spray instrument with sanitizing agents. Refer to 

Cleaning/Sanitizing, 

p .18

 for sanitizing information.

•  Use only authorized accessories (Charging Station).
•  If the instrument is broken or dropped, Do not try to disassemble the instrument. Go to 

SigmaAldrich.com/techservice

 for assistance.

BATTERIES

Battery is not user accessible or user replaceable. For more information, go to 

SigmaAldrich.com/techservice.

 Do not use instrument in a humid 

and/or corrosive environment.
•  Do not disassemble, pierce or modify the batteries or subject them to unnecessary shocks that would risk damaging them.
•  Do not leave near or in direct contact with a heat source (risk of leakage and/or explosion which may cause injuries and/or damage).
•  In the event of leakage and direct contact with the fluid, rinse the exposed area with plenty of water and seek medical  

advice immediately.

•  Do not put the batteries in your mouth. If swallowed, seek medical advice or contact the nearest poison control center immediately.
THE LITHIUM-ION BATTERY OF YOUR INSTRUMENT CONTAINS SUBSTANCES THAT MUST BE RECYCLED. DO NOT DISCARD WITH 

COMMON SOLID WASTE AT END OF LIFE. PLEASE DISPOSE OF IT IN A RECYCLING BIN SUITABLE FOR THIS TYPE OF PRODUCT. 

SEGREGATE WITH OTHER WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) IN THE EUROPEAN UNION, PLEASE VISIT 

SigmaAldrich.com/weee

.

SYSTEM DISMANTLING – WEEE directive

In accordance with European Union directive on the management of waste electrical and electronic equipment (WEEE), 

this product must not be disposed of in unsorted municipal waste at the end of its life. It must be taken to a collection 

and recycling center. For further information, go to  

SigmaAldrich.com/weee

.

ANTENNA

Unauthorized modification of the built-in antenna or use of unauthorized accessories might damage the system and render it  

non-compliant with directives such as the EU RED directive and FCC regulations. This instrument complies with part 15 of the  

FCC Rules. Operation is subject to the following two conditions: (1) This instrument may not cause harmful interference, and  

(2) this instrument must accept any interference received, including interference that may cause undesired operation.

Summary of Contents for PHCC30000

Page 1: ...perates as MilliporeSigma in the U S and Canada The life science business of Merck KGaA Darmstadt Germany operates as MilliporeSigma in the US and Canada SigmaAldrich com User Guide Scepter 3 0 Handhe...

Page 2: ...r computer easily No more cables The new Charging Station stores Scepter 3 0 out of the way when not in use freeing up valuable hood or lab bench space This system is intended for research use only Co...

Page 3: ...nstrument to vibrations Vibrations may cause instrument malfunction or damage Do not autoclave or expose to high temperature To avoid damage to internal electronic components do not spray instrument w...

Page 4: ...s the sensor Tech Service Port For Service use only USB Port Connects instrument to USB thumb drive Ergonomic Hook Allows comfortable handling of the instrument Secures the instrument to charger Contr...

Page 5: ...the Recharging screen when placed in the Charging Station the following steps must be followed to start charging the Scepter 3 0 battery 1 Place Scepter 3 0 into the Charging Station 2 While on the s...

Page 6: ...kable surface 1 Use double sided mounting tape 2 Remove tape liner from one side of tape and affix to back of mounting plate as shown below 3 Remove remaining tape liner and press firmly onto glass me...

Page 7: ...14 16 Edit Time Hours Minutes Seconds 14 16 34 Press OK to accept 01 Feb 18 14 16 Edit Time Hours Minutes Seconds 14 16 34 Press OK to accept 1 Scroll to Edit Time and press the OK button 2 Scroll to...

Page 8: ...nd select Properties If the File system is identified as FAT32 no formatting is needed Continue to Creating a Wi Fi Configuration File NOTE This view will also show how much space is available on your...

Page 9: ...file from the computer onto the USB thumb drive Once the file has loaded remove the USB thumb drive from the computer port Connect to Wi Fi Network 1 From the Main Menu select Settings and press the...

Page 10: ...were last set by the user either during a count or during post count data management 01 Feb 18 14 16 Menu Home Histograms Settings Help 01 Feb 18 14 16 Settings Back Date Time Bluetooth Wifi Gating M...

Page 11: ...epter 3 0 instrument Do not use sensor if visibly damaged Attach the Sensor 1 When the Scepter 3 0 is powered on it will show Attach the Sensor to begin 2 Holding the sensor with the sensor size numbe...

Page 12: ...net is recommend use low vacuum 5 Hg Cell Bead and Particle Suspensions Materials Required Cell sample Scepter 3 0 Test Beads Cat No PHCC3BEADS or particle suspensions Note Some particle suspensions...

Page 13: ...ion and diameter or volume will be displayed on the screen Press the OK button to save up to 999 histograms in the Scepter 3 0 memory and open the Histogram Explorer Press the OK button to return to H...

Page 14: ...03 Dec 17 Count 0 900 36 24 30 18 12 6 Dia in m 3 342e5 15 685 mL m Conc Dia 01 Feb 18 14 16 Histogram Edit Gates High gate Done 26 575 Low gate 12 585 Count 0 900 36 24 30 18 12 6 Dia in m Switch to...

Page 15: ...wirelessly Materials Required Wireless Network broadcasting at 2 4 Ghz PC Mac or UNIX Computer connected to the same Wi Fi Network as Scepter 3 0 Scepter 3 0 Instrument Follow Wi Fi Network p 8 direct...

Page 16: ...MS Press the OK button from any histogram on screen to open the Histogram Explorer menu 01 Feb 18 14 16 Menu Home Histograms Settings Help 01 Feb 18 14 16 Histogram Test 3 03 Dec 17 Count 0 900 36 24...

Page 17: ...o printer Help Screens Tutorials 1 From the Menu screen scroll to Help press the OK button 2 Scroll to the topic of interest press the OK button 01 Feb 18 14 16 Scepter Software 4 6 01 Feb 18 14 16 Me...

Page 18: ...ration of cell sample is within recommended operating range See Cell Bead and Particle Suspensions Test sample using Scepter 3 0 Test Beads Cat No PHCC3BEADS to ensure unit operating properly for conc...

Page 19: ...y needs to be re charged Place Scepter 3 0 on charging stand to recharge the instrument See Battery p 5 Battery is not charging Ensure that the unit is correctly positioned in charging stand An animat...

Page 20: ...ressing of OK button Fully submerge sensor in sample before pressing OK button Air bubble in sample Mix sample gently to avoid air bubbles Sensor lifted from sample before screen displays Sample Loade...

Page 21: ...ed on FAT32 formatted USB thumb drive and try again See USB Thumb Drive Formatting p 8 Software update blocked on battery low Insufficient battery to perform update Recharge Scepter 3 0 and try again...

Page 22: ...ntal Conditions Temperature 15 C to 25 C Maximum Relative Humidity 20 80 non condensing Storage Conditions Temperature 15 C to 30 C Maximum Relative Humidity 20 80 non condensing Scepter 3 0 Sensor Ma...

Page 23: ...p E GP Sterile Vacuum Filtration System Millipore Express PLUS PES 0 22 500 38 12 SEGPU0538 0 22 500 45 12 SEGPU0545 0 22 1000 38 12 SEGPU1138 0 22 1000 45 12 SEGPU1145 Steritop E GP Sterile Funnel le...

Page 24: ...Merck KGaA Darmstadt Germany and or its affiliates All Rights Reserved Global Registration Marks of Conformity USA TFB 1003 EUROPE C TAIWAN JAPAN R209 J00212 SOUTH KOREA R C Emd PHCC30000 RUSSIA BELAR...

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