Revision 21_Sep_2015
34
User Guide Version 2.60 I-button WHO 5th
APPENDIX 3: Using Standard Slides in the Visualization System
Introduction
The SQA-V has a specially designed slide adaptor that enables the user to use standard slides to view
semen samples in the SQA-V visualization compartment. A slide is "seated" in a stable and secure manner
as described below and the slide adaptor is inserted into the SQA-V for testing.
User Instructions:
1.
The slide adapter is designed for standard laboratory slides that are 76 mm long and 25.6 mm
wide. Thickness may vary from 1 mm to 2 mm. The viewing section of the slide must be completely
transparent.
2.
Center a 10 micro-liter drop of semen at a distance of approximately 12 mm from the edge of the
slide and cover with a standard (22 mm x 22 mm) cover-slip. The droplet of semen should be
evenly spread across the entire surface area of the cover-slip automatically, without any additional
pressure applied to the cover-slip:
Sample location
3.
Carefully place the prepared slide into the
slide adapter (with the non-loaded side
towards the slide holder):
4.
Open the spring loaded slide holder by
pressing on its outer edge. Slip the slide into
the holder and release the spring:
5.
Align the edge of the slide with the distal
edge of the slide adapter by turning the
silver slide position adjuster as seen below.
The slide will now be firmly in place in the
slide adapter:
6.
Insert the fully loaded slide adapter into the
visualization chamber of the SQA-V:
7.
Optimize the video image in the usual
manner (please see the SECTION 9:
Operating the Visualization System) and
move to additional fields of view by turning
the silver knob of the slide adapter.
