User Guide Version 2.60 I-button WHO 5th
Revision 21_Sep_2015
19
Post
Vasectomy
Testing
Postvasectomy Test
The SQA-V runs a five minute POSTVASECTOMY test that can detect the presence of a
very small number of motile cells. Once the automated test has been performed, the
user is given the option to follow the POSTVASECTOMY protocol outlined below and
"scan" the testing capillary in the SQA-V visualization system (A POSTVACECTOMY
Protocol can also be found in the appendix section of this guide).
By scanning through the depth of the testing capillary, immotile and motile sperm cells
can be readily identified, easily counted and entered in the operational screen for visual
confirmation of the automated test results. Clinical studies positively demonstrated that
by incorporating both the SQA-V automated AND visualization system in the testing
protocol, a very high level of accuracy is obtained for identifying motile and non-motile
sperm cells in POSTVASECTOMY samples.
In order to obtain similar levels of accuracy it is imperative that the user strictly follow
the manufacturer's protocol outlined below. Additionally, once the testing cycle is
completed, test results can be documented by capturing and archiving a video clip of the
postvasectomy specimen using V-Sperm™ software.
Select
POSTVASECTOMY
as the SAMPLE TYPE from the ENTER PATIENT / SAMPLE
DATA screen.
Fill the SQA-V testing capillary following instructions in the appendix section of
this guide: "Filling the SQA-V Capillary with a Normal Volume Sample."
Please note:
The POSTVASECTOMY
test takes
approximately 5
minutes to run and is
highly sensitive to
motion. Please do not
disturb the SQA-V or
the testing capillary
during the testing cycle
or the results may be
impacted.
Insert the testing capillary into the SQA-
TESTING
V lower chamber when instructed.
Testing will begin automatically.
DO NOT MOVE CAPILLARY OR
Testing takes approximately 5 minutes.
OPERATE DEVICE DURING TESTING
Test results for motile sperm are
reported.
THIS TEST TAKES APPROX. 5 MINUTES
Select
YES
to when asked: “ENTER
VISUAL DATA PER USER GUIDE?” to
POSTVASECTOMY
manually enter the number of
# SPERM/SCAN: # SPERM/SAMPLE VOL.:
MOTILE/IMMOTILE sperm seen on the
MOTILE
3
MOTILE
0.2 M
visualization system.
Press
ENTER
to continue.
ENTER VISUAL DATA PER USER GUIDE?
YES
/NO
Take the same testing capillary and
insert it into the visualization (upper)
compartment.
Set the magnification to x300 (Full zoom
PLEASE INSERT CAPILLARY
out) and press ENTER to continue.
INTO VISUALIZATION SLOT
ADJUST MAGNIFICATION TO x300
"Scan" the depth of the capillary by
slightly turning the visualization focus
PRESS ENTER
knob (10 fields can be visualized) and
enter the total
# MOTILE/IMMOTILE
SPERM cells
visualized in all 10 fields.
TURN FOCUS KNOB AND SCAN THROUGH
ENTIRE CAPILLARY DEPTH TO COUNT
The SQA-V will automatically report the
MOTILE AND IMMOTILE SPERM
GREATER # of cells found by the
PLEASE ENTER:
Automated or Visualization system.
# MOTILE SPERM
3
Press
ENTER
and the test results screen
# IMMOTILE SPERM
8
will be displayed.
