User Guide Version 2.60 I-Button
Revision 21_Sep_2015
10
Technology
SECTION 3: Technology
SQA-V Optical Block
Density
Motility
Step 1
Detector
Detector
OD
Conv
Micro
Processor
M.E.S.
Proprietary
Algorithms
Step 2
Step 3
Conv
Semen
Parameters
LED
LED
Step 1:
The capillary is inserted into the measurement compartment.
Step 2:
Concentration:
Millions of sperm cells are analyzed: A very specific wavelength of light
is absorbed by the sperm cells in the concentration chamber of the
SQA-V testing capillary.
An optical density detector measures the amount of light absorbed by
the cells and converts it to optical density (OD).
The “OD” reading is translated into sperm concentration by a
microprocessor based on proprietary MES algorithms.
Step 3:
Motility:
Tens of thousands of sperm cells are analyzed in the thin section of the
SQA-V capillary as they move through a light beam in the SQA-V: The
movement of motile sperm cells causes light disturbances.
These light disturbances are converted into electronic signals with
“peaks and valleys.”
The electronic signal peaks are analyzed by microprocessor software
based on a proprietary MES algorithm and translated into motility
parameters.
0.8
0.6
0.4
0.2
0
-0.2
-0.4
-0.6
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
2
Time [sec]
Electronic Signal of Motile Sperm
V
ol
tage
[v
ol
ts
]
