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Storage and Disposal

9. Storage and Disposal

9.1 Storage

The incubator may only be used under the following conditions.

Dry and in an enclosed, dust-free room

Frost-free

Disconnected from the power supply and gas supply

Undo gas bottle connections and close valves of gas bottles.

Gas bottles may be stored in closed rooms if these are sufficiently well ventilated.

For appliances with basic fittings:

Remove, empty and clean the water tray(s).

For appliances with the humidity module:

Undo hose connection of water supply tank; empty water tank.

9.2 Disposal

This product is subject to the Directive 2002/96/EC on Waste Electrical

Electronic Equipment (WEEE) of the European Parliament and of the EU

Council of Ministers. This appliance has been brought to the market

after 13th August 2005 in countries which have already integreated

this directive into their national laws. It may not be disposed of in

normal household waste. To dispose, please contact your dealer or

manufacturer. Any appliances that are infected, infectious or contami-

nated with materials that are a hazard to health are excluded from

being taken back. Please observe the other regulations in this context.
Before disposing of the appliance, please render the door locking

mechanism unusable, for example, to prevent playing children from

being locked inside the appliance.
Note for Germany:

The appliance may not be left at public or communal recycling or collection points.

Summary of Contents for INCO 108

Page 1: ...OPERATING MANUAL CO2 incubators INCO 108 INCO 108 med INCO 153 INCO 153 med INCO 246 INCO 246 med ...

Page 2: ...Fon 49 0 09122 925 0 Fax 49 0 09122 14585 E Mail sales memmert com Internet www memmert com Customer service Fon 49 0 09122 925 128 and 49 0 09122 925 126 E Mail service memmert com For service enquiries please always specify the appliance number on the nameplate see page 15 2014 Memmert GmbH Co KG Edition 11 2014 Subject to modifications ...

Page 3: ...ic model and with six different additional modules The technical fittings and functional range of the basic model and the individual additional modules are described from page 11 If specific equipment features or functions are available only with one of the additional mod ules this is pointed out in the relevant sections in this manual Due to individual fittings depictions in this manual may be di...

Page 4: ...ation nameplate 15 2 10 Technical data 16 2 11 Ambient conditions 18 2 12 Accessories included 18 3 Delivery Transport and Setting Up 19 3 1 Safety regulations 19 3 2 Transport 19 3 3 Delivery y 19 3 4 Setup 20 4 Putting into Operation 22 4 1 Checks 22 4 2 Connecting 22 4 3 Oxygen calibration 24 5 Operation and control 25 5 1 Operating personnel 25 5 2 Opening the door 25 5 3 Loading the incubator...

Page 5: ... chipcard 49 7 5 User ID card optionally available as an accessory 50 7 6 Calibration 50 7 7 Communication interfaces 55 7 8 Log memory y 58 8 Maintenance and Servicing 59 8 1 Cleaning 59 8 2 Regular maintenance 59 8 3 Adjusting door r 60 8 4 Repairs and Service e 60 9 Storage and Disposal 61 9 1 Storage e 61 9 2 Disposal 61 Index 62 ...

Page 6: ...observe the accompanying safety regulation Caution is used for information that is important for avoiding damage 1 1 2 Icons used Prohibited sign forbidding an action Do not tilt appliance Do not lift appliance Warning icons warning of a danger Danger of electrical shock Explosive atmosphere Danger of frost bite cold burns Warning of gas bottles heat hot surfaces Gas Regulation signs stipulate an ...

Page 7: ...heated up see also Chapter 2 7 Intended use on page 14 Warning Danger of suffocation In high concentrations CO2 and N2 can have a suffocating effect In normal operation the incubator gives off small amounts of CO2 and if equipped with the O2 module N2 to its environment You should therefore ensure that the room in which it is installed is properly ventilated If a gas bottle is not con nected or is...

Page 8: ...staff acordingly Ź is responsible for ensuring that unauthorised persons have no access to the incubator Ź is responsible for ensuring that the maintenance plan is adhered to and that maintenance and repair work is properly carried out see page 59 Ź ensures for example through corresponding instructions and inspections that the incuba tor and its surroundings are kept clean and tidy Ź is responsib...

Page 9: ...include a loss of mobility and consciousness The victim is not aware of suffocating Low concentrations of CO2 can cause accelerated breathing and headaches Anyone affected should breathe fresh air using a breathing device independent of recircu lating air Keep the person warm and calm Call a doctor In case of respiratory arrest use artificial respiration If gas is escaping g p g Leave the room imm...

Page 10: ...key 3 Push turn control y 4 Connections on the rear of the oven see page 13 3 5 Heating ribs see page 11 6 Pt100 temperature sensors 7 Humidity sensor p 8 Glass door y 9 Chamber seal 10 Sliding shelf 11 Door seal g 12 Ventilator inlet opening 13 Heating ribs 14 Adjustable feet g g 15 Nameplate beneath door see page j 15 5 16 Door knob see page p p 25 5 17 Door 18 Chip card reader ...

Page 11: ...set rate The sterile hot air is let into the interior above the fan and is mixed with the air current 5 1 1 1 1 1 1 4 4 2 5 3 1 Fig 3 How INCO incubators work 2 3 Optional extras 2 3 1 Basic equipment Ź Electronic fuzzy supported PID process controller with pulse width modulation and per manent performance adjustments and time saving self diagnosis system to quickly locate errors see page 39 Ź All...

Page 12: ...n the device without a sepa rate PC see from page 50 2 3 2 Optionally available additional modules Comfort module Ź Two gas connections with quick release connectors see page 13 Ź Automatic switch over of gas bottles Hygiene module yg Ź Electro polished seamless laser welded chamber Communication module Ź Logging option of temperature CO2 and relative humidity via computer laptop Ź Internal log me...

Page 13: ...EN 61010 Ź Protection type IP 20 acc to EN 60 529 Ź Interference suppressed acc to EN 55011 class B Ź Appliance fuse Fusible link 250 V 15 A quick blow Ź The temperature controller is protected with a miniature fuse 100 mA 200 mA at 115 V Ź For models with a humidity model the CO2 controller is protected with a 6 3 A miniature fuse 2 6 Connections 3 4 5 6 2 1 H2 O 7 N2 In Fig 4 Connections on rear...

Page 14: ... pressure must not exceed 1 2 bar A value between 0 8 and 1 bar is considered to be an ideal value 2 6 4 Water connection For models with a humidity module the incubator can be connected with the supplied hose to the also supplied water supply tank 2 7 Intended use INCO incubators may only be used for incubating cell cultures or similar Any other use is improper and may result in hazards and damag...

Page 15: ...ins 2 8 EC Declaration of Conformity You can download the EC declaration of conformity of the appliance online English http www memmert com en service downloads ce statement German http www memmert com de service downloads eg konformitaetserklaerung 2 9 Designation nameplate The nameplate Fig 5 provides information about the appliance model manufacturer and technical data It is attached to the fro...

Page 16: ...lf kg 15 15 15 Max load per appliance kg 40 40 60 Temperature Temperature recording by means of Pt100 in a 4 wire circuit Adjustment range Normal mode 20 C to 50 C Sterilisation mode 160 C 4 hours via STERICard Adjustment precision 0 1 C Control range from 8 C above room temperature to 50 C Variation time max 0 1 C at 37 C Variation spatial max 0 3 C at 37 C Humidity The relative humidity in the c...

Page 17: ...0 to 20 Ź Adjustment precision 0 1 Ź Variation time max 0 1 rh Ź Variation spatial max 0 3 O2 only if equipped with O2 module The O2 content is determined with a long lasting mainte nance free zirconium dioxide sensor constantly controlled by a microprocessor and displayed digitally in percent Ź Adjustment range 1 to 20 Ź Adjustment precision 0 1 Ź Variation time max 0 1 Ź Variation spatial max 0 ...

Page 18: ...dimentation in the chamber interior and as a consequence could result in short circuits or damage to electrical parts For this reason sufficient measures should be taken to prevent large clouds of dust or aggressive vapours from developing 2 12 Accessories included For incubators with basic fittings g Ź Two INCO 108 or three INCO 153 and 246 sliding shelves Ź Gas pressure hose Ź Water tray one for...

Page 19: ... 2 Transport The incubator can be transported in three ways Ź with a fork lift truck move the forks of the truck entirely under the incubator Ź on the manual pallet jack Ź by carrying to do this at least two people are required for models 108 and 153 and four people for model 246 Read the weight information detailed on page 16 3 3 Delivery The incubator is delivered in cardboard packaging on a car...

Page 20: ...y carry the weight of the incubator see page 16 Do not place the ap pliance on an inflammable surface A power connection must be available at the installation site in accordance with the connec tion data on the nameplate see page 15 The distance between the wall and the rear of the chamber must be at least 15 cm The clear ance from the ceiling must not be less than 20 cm and the side clearance fro...

Page 21: ...ched to the lower oven Fig 9 1 Remove the housing cover from the lower oven 2 Insert drilling template supplied with the foot alignments into the overturned lid 3 Mark drilling points and drill with a 4 2 mm diameter drill bit 4 Screw the foot alignment provisions to the top of the lid with the screws and nuts sup plied 5 Put the covers back on Fig 8 Base Fig 9 Assembly of the foot alignment provi...

Page 22: ...gy supply company what the system impedance is Connect power cable see Fig 4 on page 13 4 2 2 External devices only for models with communication or premium modules Only appliances whose interfaces comply with the requirements for safety extra low voltge e g PC laptop printer may be connected to the connections on the rear of the incubator see Fig 4 on page 13 Which devices may be connected depend...

Page 23: ...ames in the vicinity of the gas bottles Store gas bottles at lower than 50 C in a well ventilated location Prevent water from penetrating as well as backflow into the gas bottles It is essential that you read the safety notes and regulations of the gas suppliers For incubators with basic fittings g Attach the supplied pressure hose to the gas bottle pressure regulator and to the CO2 connection on ...

Page 24: ...with a hose clamp Fig 13 see also page 13 Ź Attach push on the supplied N2 pres sure hose to the N2 gas bottle pressure reducer and to the N2 In connection on the rear of the incubator with a hose clamp 4 3 Oxygen calibration only for models with O2 module Perform an O2 calibration before putting into operation see page 54 CO2 N2 CO2 In N2 In Fig 13 Gas connection for incubators with O2 mod ule ...

Page 25: ...ith the materials of the incubator see page 13 since considerable damage could otherwise occur to the chamber load the appliance or the surroundings The incubators are not explosion proof they do not comply with workplace health safety regulation VBG 24 and are therefore not suitable for drying vaporising and branding paints or similar materials the solvents of which could form an explosive mixtur...

Page 26: ... 1 5 153 1 1 5 to 2 5 1 to 1 5 246 2 1 5 to 2 5 1 to 1 5 5 5 Connect gas supply 1 Check that the gas bottle s is properly connected see also page 13 2 Open valve s 5 6 Switch on appliance The incubator is switched on and off by pressing the main switch push turn control on the front of the appliance Ź Switching on press the main switch so that it comes out of the appliance Fig 16 Ź Switching off p...

Page 27: ...g 11 Gas bottle 1 active y p y y 12 Gas bottle 2 active 13 CO2 display 14 Display appliance is humidifying 2 p y 15 Operating mode display see page p y pp y g 28 8 16 Text display O p g g 2 display O p y p 2 display only if p g p g equipped with O p y y 2 2 module p y 2 All operating functions are selected by turning the push turn control to the left or right and adjusted by turning this with the ...

Page 28: ... 29 Ź Week time switch The incubator runs at the set values only at certain times Operation in this mode is described from page 31 Ź Interface mode with PC laptop for models with communication or premium modules see from page 55 In addition basic appliance settings can be made SETUP see page 43 and printouts can be made if the appliance is equipped with the communication or premium module PRINT se...

Page 29: ... turn control and the set key Temperature setpoint p p Adjustment range 20 C to C 50 C C Temperature monitoring p g Adjustment range MIN MAX AUTO see also page 45 C MIN AUTO MAX Humidity setpoint y p Adjustment range Ź For incubators with basic fittings 88 to 97 rh Ź For models with humidity module 40 to 97 rh rh High levels of air humidity in the interior can only be achieved without condensation...

Page 30: ...he temperature setpoint Hold down the SET key and set the desired temperature setpoint of 37 0 C with the push turn control Release the SET key the appliance will briefly flash show ing the temperature setpoint Then the current tempera ture appears on the display and the controller begins to move to the set temperature of 37 0 C Ź Heating up is indicated by the icon C 3 Setting the monitoring temp...

Page 31: ...on the display and the controller begins to move to the set CO2 setpoint Depending on the bottle used fumigation is dis played by the IN 1 or IN 2 icon CO2 6 Adjust the j O2 setpoint p only for models with O2 module Turn the push turn control to the left until the O2 display flashes Hold down the set key and set the desired O2 setpoint of 3 0 with the push turn control Release the set key The appl...

Page 32: ...c are made for the ON phase the controller takes over the values from the normal operating mode For reasons of safety you should always check that only one switch one time is programmed in the desired time blocks and days By turning further to the right parameters temperature setpoint etc can be selected as in the normal operating mode If the controller is in standby mode or the week time switch i...

Page 33: ...e current operating mode then begins to flash Select the week time switch operating mode with the push turn control while the SET key is held down Release the SET key the control is now in the week time switch operating mode PRINT SETUP 2 Switch on Mo Fr at 09 30 Turning the push turn control to the left select the Mo Fr on icons group working days Hold down the SET key and set the desired switch ...

Page 34: ...level If necessary add distilled water Warning messages during operation See Page 38 CO2 2 mode In the heating up phase the CO2 controller is initially deactivated The CO2 intake is inter rupted during this period About 5 minutes after the setpoint temperature has been reached the CO2 control begins measuring and CO2 gas is let in to the chamber via a sterile filter the valve on the gas bottle mus...

Page 35: ...a constant approx 57 bar at 20 C ambient temperature It is not possible to determine how full the bottle is through the pressure since the pressure only drops immediately before the bottle is com pletely empty The CO2 supply is automatically interrupted when the outer doors are opened door open is indicated in the text display on off 2 e m p t y 2 supply is interrupted In this case check if the N2...

Page 36: ...hed on Ź after every change of the CO2 setpoint Humidity limit control y A humidity limit control prevents the formation of condensation water in the chamber and at the same time ensures that the setpoint humidity is quickly reached with short recovery times The maximum achievable humidity can be adjusted in the standard model from 88 to 97 rh Active humidity control only for models with humidity ...

Page 37: ...Fig 22 2 Close the valve s of the gas bottle s 3 Open the door see page 25 4 Remove the chamber load 5 For appliances with the basic model Remove and empty the water trays Fill water trays and insert them only when the appliance is next used For incubators with a humidity module empty the water tanks if the incubator is not used for several days Fig 22 Switching off incubator ...

Page 38: ...tact the customer service hi alarm overtempera ture alarm limit max ex ceeded if overtemperature protection responds Remedy Check the setting of the MAX temperature monitor ing see Chapter Temperature monitoring on page 45 undertem perature alarm limit min crossed if undertempera ture protection responds Remedy Check the setting of the MIN temperature monitor ing see Chapter Temperature monitoring...

Page 39: ...curs again Contact the customer service Error in O2 2 system y on off Mo Tu We Th Fr Sa Su 2 e m p t y 2 supply is defective Remedy check if the N2 bottle is correctly connected and if the valve is open If that does not solve the problem you should connect a new gas bottle see page 23 6 2 System appliance errors Warning After removing covers live parts may be exposed You may receive an electric sh...

Page 40: ...ture difference between monitoring and working temperature see page 45 Ź Replace Pt100 temperature sensor of monitoring controller if necessary see Service manual and rh empty Water supply tank empty Set humidity setpoint to OFF fill up distilled water then reset humidity setpoint back to desired value and rh empty Humidity setpoint exceeded Open door for 30 sec and wait to see if subsequently the...

Page 41: ...read the service manual Heating icon not on Ź Ambient tempera ture too high Setp the appliance up in a cooler room minimum setpoint temperature am bient temp 8 C Ź Temperature in appliance higher than set setpoint temperature Wait until appliance has cooled down icon is permanently lit up Temperature fuse has responded Ź Switch off appliance and leave to cool down Ź Check temperature limiter The a...

Page 42: ...f the power failure is documented in the log memory For remote operation p If there is a power failure in remote operation the appliance starts in the normal operating mode for safety reasons and all setpoint values are changed to safe default values see table The programme can only be continued from the computer The time and duration of the power failure are documented in the log memory Parameter...

Page 43: ...eader is also printed automatically containing the following details Ź Date of printout Ź Period of log Ź Consecutive page numbers Ź Serial numbers and appliance name Running the printing function g p g Select the PRINT operating mode with the push turn control as described on page 28 By turning the push turn control and holding down the SET key the following parameters can be selected and altered...

Page 44: ... for the different lengths of months and for leap years Weekday Tu Year Adjustment range from 2000 to 2100 Acoustic signal at programme end g p g ENDSOUND Setting OFF or F ON Acoustic Signal for alarm e g over undertemperature g g p ALARM so Setting OFF or F ON Communication address Adjustment range 0 to 15 see Chapter Communication interfaces on page 55 Adjustment range 0 5 bis 5 C see page 48 La...

Page 45: ... to protect the chamber load and as a protection for the appliance and surroundings The appliance has a double overtemperature fuse mechanical electronic in accordance with DIN 12880 C MIN AUTO MAX 1 2 3 1 6 5 4 Fig 23 Temperature monitoring display 1 Visual alarm icon p Lit up TB alarm Flashing TWW alarm ASF alarm p p 2 Undertemperature limit g g 3 Automatic temperature monitor ASF see page 48 8 ...

Page 46: ...adjustable over undertemperature controller TWW protection class 3 3 acc to DIN 12880 If the manually set monitoring temperature MAX is exceeded the TWW takes over tempera ture control and begins to regulate the monitoring temperature Fig 21 The alarm icon flashes as a warning Setting MAX Set temperature t C Controller error Emergency operation Fig 24 Schematic diagram of how the TWW temperature m...

Page 47: ...set the desired temperature limit with the push turn control e g 38 5 C C MIN AUTO MAX Undertemperature limit Adjustment range 10 C below the minimum temperature of the appliance up to 10 C above nominal temperature of the appli ance for details of the nominal temperature see nameplate The lower alarm limit value cannot be set higher than the top one If no undertemperature limit is required set th...

Page 48: ...arily switched off until the next alarm event occurs The ASF alarm goes off automatically as soon as 50 of the set tolerance band of the set point in the example 37 C 1 5 C are reached again section C If the temperature setpoint is altered the ASF is automatically disabled temporarily in the example The setpoint is changed from 37 C auf 30 C section D until it has reached the tolerance range of th...

Page 49: ...sterilised To activate the STERICard insert it into the chip card reader see page 10 on the operating panel of the appliance and select the Start icon with the push turn control while the SET key is held down As soon as the sterilisation procedure has been enabled the automatic sterilisation procedure begins Ramp 1 Heating up to 160 C Ramp 2 Hold time 4 hours after setpoint temperature has been re...

Page 50: ...ery login process with the user ID card is logged in the internal Flash memory of the control ler 7 6 Calibration 7 6 1 Temperature calibration The incubator can be calibrated customer specifically using three calibration temperatures of your choice Ź CAL 1 Temperature calibration at low temperature to 30 C Ź CAL 2 Temperature calibration at medium temperature from 30 C to 38 C Ź CAL 3 Temperature...

Page 51: ...f necessary Example The temperature deviation in the chamber load at 35 C should be corrected 1 Set the compensation temperature CAL 2 in the SETUP to 35 0 C and the accompanying compensation correction value to 0 0 C h C C 2 With a calibrated reference instrument and at a set setpoint temperature of 35 C in nor mal operation an actual temperature of 34 6 C is measured 3 Set compensation correctio...

Page 52: ...If the measured reference humid ity is too low the compensation correction value must be set with a negative sign 4 Perform a control measurement with the reference instrument The procedure can be performed with humidity balance points of 20 rh and 90 rh Example Humidity deviation at 90 should be corrected 1 Set humidity balance point in the SETUP to RH 90 and set the accompanying compensation cor...

Page 53: ...lected balance point a positive or negative compensation correction value can be set co2 5 0 4 20 0 10 co2 10 1 5 co2 15 0 7 Default calibration Fig 30 CO2 calibration example Setting g 5 Set the desired CO2 balance point in the SETUP see page 44 and set accompanying compensation correction value to 0 0 rh 6 With a reference instrument measure the deviation in the stationary state in the selected ...

Page 54: ...6 4 Oxygen calibration only for models with O2 module In the O2 calibration the oxygen proportion in the incubator at normal atmosphere is deter mined 1 Switch off the O2 module if active To do this turn the push turn control to the left until the O2 display flashes Hold down the set key and turn the push turn control until O2 off is displayed Release the set key 2 Set CO2 setpoint value to 0 see ...

Page 55: ...this address the ap propriate incubator can be selected from the computer 7 7 1 Communication Interface RS 232 C in accordance with DIN 12900 1 The computer can be connected with a shielded interface cable to the 9 pin interface on the rear of the appliance see Fig 31 and page 13 The shielding must be connected to the plug casing If the serial port is not used put on the cover included If several ...

Page 56: ...th the oven A maximum of 16 devices can be addressed on the RS 485 bus The default setting is ADDRess 0 Using this the appropriate incubator can be selected and programmed from the PC The computer needs to be equipped with either an RS 485 interface or with an RS 232 RS 485 converter Fig 34 Connecting the wires is done individually with a shielded cable depending on the installa tion site The maxi...

Page 57: ...ected to a computer via USB interface an appropriate interface on the computer and a separate cable are required for each chamber The maximum cable length is 5 m 7 7 4 Ethernet interface LAN 1 192 168 1 233 192 168 1 216 LAN 2 192 168 1 215 LAN 3 192 168 1 241 Fig 35 Connecting one or more incubators to a network using an Ethernet interface schematic diagram g g g ...

Page 58: ...fe from manipulation Each dataset is stored with a unique times tamp The internal log memory has a size of 1024 kB This corresponds to a storage capacity for about three months in permanent operation If the power supply is interrupted the time of the power cut and the return of voltage are stored in the controller Reading in the log memory to the PC via USB interface g g y For documentation purpos...

Page 59: ...r with the stainless steel housing Rust deposits lead to an infection of the stainless steel If rust spots should appear on the surface of the working chamber due to impurities immediately clean and polish the affected area Do not clean the operating panel the plastic input module and other plastic parts of the chamber with caustic or solvent based cleaning agents 8 2 Regular maintenance Monthly y...

Page 60: ...with locking paint 2 Adjust the door by turning the eccentric 3 with a screwdriver 3 Apply locking paint to the maggot screw and re tighten it The locking plate Fig 39 can also be adjusted in the direction of the arrows after undoing the screw 6 1 Undo screw 6 2 Move locking plate 7 in direction of arrow 3 Tighten the screws again 8 4 Repairs and Service Warning After removing covers live parts ma...

Page 61: ...n Waste Electrical Electronic Equipment WEEE of the European Parliament and of the EU Council of Ministers This appliance has been brought to the market after 13th August 2005 in countries which have already integreated this directive into their national laws It may not be disposed of in normal household waste To dispose please contact your dealer or manufacturer Any appliances that are infected i...

Page 62: ...ng operation 37 ENDSOUND 44 Errors 39 Ethernet interface 57 Explosion protection 14 External devices 22 Extras 11 F Function 11 G Gas connection 14 Gas supply 26 H Hazards 7 How to set the IP address 58 Humidity calibration 52 53 I Icons used 6 Intended use 14 Interface cable 43 Interface mode 28 Interfaces 55 Irregularities 8 L Language 44 Laptop 34 Length of lead 57 Loading 25 Log memory 42 43 M...

Page 63: ...e 22 T Technical data 16 Temperature calibration 50 51 Temperature comparison 50 Temperature deviation 51 Temperature limiter TB 45 Temperature monitor 48 Temperature monitoring 45 Temperature monitoring TWW 46 Temperature sensor 45 Terms used 6 Tolerance band ASF 44 Transport 19 Transport damage 19 TWW 46 U Unpacking 19 USB interface 57 User ID card 50 W Warning messages 49 Water connection 22 Wa...

Page 64: ...Memmert GmbH Co KG Postfach 1720 D 91107 Schwabach Tel 49 0 9122 925 0 Fax 49 0 9122 145 85 E Mail service memmert com www memmert com 05 11 2014 INCO INCO med englisch D23532 ...

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