Symptom
Possible Causes
Recommended Solutions
Flow Core Position test
failure
See solution above (For more information, see
Flow Core Axial Stability test fail-
ure
.).
Focus Percentage test
failure
See solution above (For more information, see
Flow Core Axial Stability test fail-
ure
.).
Focus Uniformity test
failure
See solution above (For more information, see
Flow Core Axial Stability test fail-
ure
.).
Image Quality test fail-
ure
See solution above (For more information, see
Flow Core Axial Stability test fail-
ure
.).
Compensation
fails to compute
values and save
data
The region to collect is
set incorrectly
In the Acquisition Settings section
verify that 1,000 of “All” cells (or of a region drawn on the appropriate
population) are being collected.
Too many objects are
being collected
Set the events to acquire less than 1,000.
Cells are not fluor-
escent
Make sure that the compensation control sample has more than 10% pos-
itive events, and are as bright as possible. IgG capture beads or a cell line
stained with a single fluorochrome may be used for comp controls.
Cells are stained with
more than one fluoro-
chrome
Compensation controls must be a sample with a single fluorochrome label in
a single tube. Each fluorochrome must be run separately.
Cell concentration is
too low when using the
compensation wizard
Compensation controls must be running at 25 cells per second at a minimum
Software Troubleshooting
Symptom
Possible Causes
Recommended Solutions
INSPIRE
™
appears to
freeze
Camera is not running
Click Run/Setup.
If the camera is already
running
Click Stop then Run/Setup
Imaging is paused
Click Resume.
No objects in the cur-
rent image view mode
In the cell view area, select the all population.
A script is running
Wait until the script completes, or if necessary, click Abort Script to
prematurely stop the operation.
For Research Use Only. Not for use in diagnostic procedures.
46
Amnis
®
FlowSight
®
Imaging Flow Cytometer User Manual
