Chapter 1: General Information and Safety
This section covers safety information for operating the Amnis
®
FlowSight
®
Imaging Flow Cytometer. Anyone who oper-
ates the FlowSight System should be familiar with this safety information. Keep this information readily available for all
users.
The FlowSight Imaging Flow Cytometer is manufactured by Luminex Corporation and has a rated voltage of 100–240
VAC, a rated frequency of 50/60 Hz, and a rated current of 1.5 A.
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Environmental conditions: This instrument was designed for indoor use at an altitude of less than 2000 m; at a
temperature from 5°C to 25°C; and at a maximum relative humidity of 80%, non-condensing. During instru-
ment operation the ambient temperature should be maintained /- 2 °C. The mains power supply may
not fluctuate more than +/– 10% and must meet transient over voltage category (II). The instrument is eval-
uated to Pollution Degree 2.
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Noise level: The noise level of the FlowSight System is less than 70 dB(A).
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Weight: 56 kg.
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Ventilation: Provide at least 3 inches of clearance behind the instrument to maintain proper ventilation.
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Disconnection: To disconnect the instrument from the power supply, remove the plug from the socket outlet—
which must be located in the vicinity of the machine and in view of the operator. Do not position the instrument
so that disconnecting the power cord is difficult. To immediately turn the machine off (should the need arise),
remove the plug from the socket outlet.
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Transportation: The FlowSight System relies on many delicate alignments for proper operation. The machine
may be moved only by a Luminex representative.
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Cleaning: Clean spills on the instrument with a mild detergent. Using gloves clean the sample portal and
sample elevator with a 10% bleach solution. Dispose of waste using proper precautions and in accordance
with local regulations.
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Preventative maintenance: The FlowSight System contains no serviceable parts. Only Luminex-trained tech-
nicians are allowed to align the laser beams or otherwise repair or maintain the instrument. The instrument flu-
idic system is automatically sterilized after each day’s use. This reduces the occurrence of clogging. Tubing
and valves are replaced by Luminex service personnel as part of a routine preventive maintenance schedule.
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Access to moving parts: The movement of mechanical parts within the instrument can cause injury to fingers
and hands. Access to moving parts under the hood of the FlowSight System is intended only for Luminex ser-
vice personnel.
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Protection impairment: Using controls or making adjustments other than those specified in this manual can res-
ult in hazardous exposure to laser radiation, in exposure to biohazards, or in injury from the mechanical or elec-
trical components.
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FCC compliance: This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC rules. These limits were designed to provide reasonable protection
against harmful interference when the equipment is used in a commercial environment. This equipment gen-
erates, uses, and can radiate radio-frequency energy and, if not installed and used in accordance with the
instruction manual, can cause harmful interference to radio communications. The operation of this equipment
in a residential area is likely to cause harmful interference—in which case the user will be required to correct
the interference at the user’s own expense.
For Research Use Only. Not for use in diagnostic procedures.
1
Amnis
®
FlowSight
®
Imaging Flow Cytometer User Manual
