7. To prematurely stop acquisition click
. The system prompts you to either discard the acquired data or to
save the collected data in a file. The acquisition can be paused and resumed by clicking
.
8. Once acquisition finishes, either press
to load the next sample or
return the remaining sample.
NOTE: If the next sample has no nuclear dye and follows a DNA intercalating dye-stained sample, run Load a solu-
tion of 10% bleach and then PBS to ensure that residual dye does not stain the subsequent samples.
Collecting Compensation Files
The compensation samples are collected using the compensation wizard or may be collected manually by turning bright-
field and the SSC laser OFF and enabling all channels. In either case, the experimental data files may be compensated in
IDEAS
®
Software during analysis. Collect compensation files with the same laser power settings as the experimental
samples.
1. To use the compensation wizard click
to open the wizard list and select Compensation.
2. Click Load Compensation Sample to run the first compensation single-color sample if not already running.
NOTE: If sample is already running prior to launching the wizard, go directly to the next step.
3. Click Next when the sample is running. The wizard will turn the BF and SSC illumination OFF and enable all 12 chan-
nels.
For Research Use Only. Not for use in diagnostic procedures.
23
Amnis
®
FlowSight
®
Imaging Flow Cytometer User Manual
