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Leica AutoStainer XL – Automated slide stainer
APPENDIX 3
Compatible staining programs
Program 1H&E
Program 5 Hx
Counterstain
Reagent
Station
Step
Time
Exact
Step
Time
Exact
Oven
1
10:00
Y
Xylene
1
2
2:00
N
Xylene
2
3
2:00
N
100% Alkohol
3
4
2:00
N
100% Alkohol
4
5
2:00
N
70% Alcohol
5
6
1:00
N
Wash
Wash 1
7
2:00
N
Hematoxylin
6
8
5:00
Y
1
5:00
Y
Wash
Wash 2
9
2:00
N
2
2:00
N
Acid-Alcohol
7
10
0:02
Y
3
0:02
Y
Wash
Wash 3
11
3:00
N
4
3:00
N
Scott’s
8
12
3:00
Y
5
3:00
Y
Wash
Wash 4
13
3:00
N
6
3:00
N
Eosin
14
14
2:00
Y
95% Alcohol
15
15
0:30
Y
100% Alkohol
16
16
2:00
N
7
2:00
N
100% Alkohol
17
17
2:00
N
8
2:00
N
100% Alkohol
18
18
2:00
N
9
2:00
N
Xylene
Exit
19
10
