Leica AutoStainerXL Instructions For Use Manual Download Page 1

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Instructions for Use

Leica AutoStainerXL, V2.3 English – 10/2012

Order-No.:

14 0456 80101 RevC

Always keep this manual near the instrument!
Read carefully prior to operating the instrument!

Leica

AutoStainer XL

Automated slide stainer

Summary of Contents for AutoStainerXL

Page 1: ...for Use Leica AutoStainerXL V2 3 English 10 2012 Order No 14 0456 80101 RevC Always keep this manual near the instrument Read carefully prior to operating the instrument Leica AutoStainer XL Automated slide stainer ...

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Page 3: ...de tails of the present manual are not to be consid ered as warranted characteristics of our products These are determined only by the contract provi sions agreed between ourselves and our custom ers Leica reserves the right to change technical specifications as well as manufacturing pro cesses without prior notice Only in this way is it possible to continuously improve the technology and manufact...

Page 4: ...rements 14 4 2 Connection 14 4 2 1 Power 14 4 2 2 Water supply 15 4 3 Battery backup UPS optional 16 4 4 Remote alarm optional 17 4 5 Fume control system 17 4 6 Oven 17 5 Operation 18 5 1 The control panel 19 5 2 The main menu 20 5 3 Menu map 21 5 4 Edit a program 22 5 4 1 Entering steps 23 5 4 2 Erasing steps 24 5 4 3 Inserting a blank step into a program 24 5 4 4 Removing blank steps in a progra...

Page 5: ...9 7 Interrupting staining 35 5 9 8 Aborting a rack 36 6 Cleaning and Maintenance 37 6 1 Cleaning the instrument 37 6 1 1 Wash containers 37 6 1 2 Reagent containers 37 6 1 3 Slide racks 37 6 1 4 Oven 37 7 Trouble shooting 38 7 1 Instrument failures 39 7 2 Information and warnings 40 7 2 1 During staining 40 7 2 2 During editing programs 41 7 2 3 During SetUp 42 8 Warranty and service 43 APPENDIX 1...

Page 6: ...ll inquiries it is important to state the follow ing instrument type serial number The Instructions for Use for the Leica AutoStainer XL includes chapters dealing with the following subjects Chapter 1 Manual structure including Table of contents Important information on this handbook Chapter 2 Safety Read this chapter before you attempt to operate the instrument Chapter 3 Instrument features Gener...

Page 7: ...n if you are already familiar with the operation and use of other Leica products 1 2 Designated use improper use of the instrument The AutoStainer XL has been designed for staining applications in medi cine biology and industry The instrument may be operated only according to the instructions con tained in this manual Any other use is considered an improper use of the product 1 3 Qualification of ...

Page 8: ...rical measuring control regulating and laboratory de vices In order to maintain this condition and ensure safe operation the operator must observe all the instructions and warnings contained in this Instructions for Use For current information about applicable standards please refer to the CE declaration of conformity on our Internet site www LeicaBiosystems com ...

Page 9: ...au and Hematoxylin Eosin staining can be conveniently performed at the same time without reprogramming or reagent changes The AutoStainer XL incorporates all of the features which ensure high flex ibility convenienceandaboveall qualitystaining Afan forcedovenisavail able to quickly dry slides and optimized wash stations result in rapid re moval of excess reagent The minimum carryover design of the...

Page 10: ...Instructions for Use V2 3 10 2012 10 3 Instrument features Abb 1 20 19 18 17 15 14 13 12 11 10 9 8 7 1 4 3 2 6 5 16 3 1 Overview instrument ...

Page 11: ... key 15 Exit drawer 16 Slotted lid 17 Exit LED and key 18 Container map on fume filter cover 19 Lid support 20 Lid Abb 2 Rear panel 21 Water inlet 22 Drain outlet 23 Serial port 24 Power supply 25 Oven voltage selector 26 Mains switch ON OFF 27 Mains outlet 28 Feet adjustable 29 Power jumper cable 30 Power supply inlet 31 Rating and serial number plate 32 Remote alarm socket 50 V 1 A max 33 Access...

Page 12: ...ozzle for hose 14 0475 33669 1 V filter 3 4 14 0456 36101 1 activated carbon filter 14 0474 32273 1 filter cover 14 0456 35240 1 drip tray for paraffin heating inside the instrument 14 0456 35216 1 dye log block attached to the instrument 14 0456 35459 1 water supply hose with seal 14 0474 32325 1 drain hose 14 0475 35748 1 power cable USA C J 14 0411 13559 1 operating manual Leica AutostainerXL G...

Page 13: ... 26 Total number of reagent stations at least 18 Reagent container volume 450 ml Number of wash stations max 5 Oven 1 Oven chamber temperature ambient or 30 C to 65 C Incubation time setting from 0 sec up to 99 min 59 sec Load unload stations 1 each Permanent memory capacity 15 programs up to 25 program steps each Operating temperature range 15 C to 35 C Relative humidity max 80 non condensing Dim...

Page 14: ...p and drain The power requirements for the AutoStainer XL are 8 amps at 110 volts 4 amps at 240 volts The voltage selector and other internal components are set by the manu facturer to suit the country of sale The voltage selector setting must not be altered by the user The AutoStainer XL requires connection to a laboratory water tap with mains pressure fitting 4 2 Connection 4 2 1 Power Connect t...

Page 15: ...at the side to STOP The ON OFF switch at the rear of the instrument should be left ON The instrument must never be operated without the power jumper cable The instrument must be connected to an earthed mains power outlet socket only 4 2 2 Water supply Connect the water hose to the water inlet at the rear of the unit Screw the other end of the hose to the cold water tap The hose has a 3 4 inch BSP ...

Page 16: ...continue during brief mains power failures A small UPS can be connected using the power jumper lead as shown in Figure 15 The UPS should be rated at 200 VA for 5 minutes Heating in the oven will not be maintained by the UPS The UPS must be rated for use with the local mains voltage Your distributor can recommend a suitable UPS 4 Installation Fig 16 ...

Page 17: ...o put the ON STOP switch at the side of the instrument to STOP and then to ON again The remote alarm will only operate during loss of mains power if a battery backup unit is fitted Your distributor can provide connection details for the remote alarm The remote alarm connected to the instrument must be rated at less than 1 amp and a maximum of 50 Volts Connect the remote alarm to the alarm socket a...

Page 18: ...ing the Load keymust be pressed to inform the instrument to begin processing Simi larly if a rack is finished staining in the Exit drawer the Exit LED will be on The Exit key must be pressed to inform the instrument when the rack is re moved Programs can finish at any station However if the EXIT drawer is not the last step then the LCD will inform you of the station to unload from In this case the...

Page 19: ...tion keys perform the action indicated immediately above them on the fourth line of the display The arrow keys move the cursor in the direction indicated They are also used to select digits and other settings Contact with solvents use of sharp instruments or excessive force may damage the keypad The LED indicators The four LEDs are located below the arrow keys and have the following functions The ...

Page 20: ...essage long double beep operator attention required to remove a completed rack continuous tone indicates unit failure 5 2 The main menu When the AutoStainer XL is turned ON at the ON STOP switch the following Main Menu will be displayed and the instrument will give 3 beeps The modes of operation of the instrument are Stain to stain slides Edit to create view or change programs Setup to set view or...

Page 21: ...21 Leica AutoStainer XL Automated slide stainer 5 Operation 5 3 Menu map Fig 21 ...

Page 22: ...p number defines the order in which the stations are used The im mersion time is the time the rack is fully immersed in a station As there is potential for timing clashes to occur when multiple racks are present in the instrument steps which require precise timing are designated as exact in the program Immersion times at these steps are given priority and are achieved to within 1 second Racks at s...

Page 23: ...umber use the and keys to move between step 1 to 25 of the program Alternatively press F2 Next to move to the next step 5 To enter program information use the and keys to position the cursor beneath the appropriate heading Scroll through the options or alter the digits using the and keys Fill in the program details us ing the and keys to move to the next heading as each item in the step is complet...

Page 24: ... a blank step into a program This function is used to insert an additional step into an existing program 1 Select the program see steps 1 and 2 page 22 2 Press F2 Edit 3 Select the step number where the new blank step to be inserted 4 Press F1 More 5 Press F1 More 6 Press F1 Insert 7 If you wish to proceed press F1 Yes A blank step will be inserted at the step selected in 3 The steps following the...

Page 25: ...the program see page 24 5 4 5 Saving a program When the program is complete to save it permanently 1 From the Edit Program screen press F4 Quit You now have the options of saving the edited program F1 leaving the program as it was before the changes were made F2 or continuing editing F4 2 Press F1 to save the program or 3 Press F2 to leave the program unchanged or 4 Press F4 to continue editing th...

Page 26: ...nd keys select the program number to be copied into 4 Press F1 Copy If the program number selected is not empty an informative message will be given If the program selected is assigned to a rack currently being stained the copy is not allowed and an informative message will be given A confirmatory message will be momentarily displayed if the copy is suc cessful 5 If you wish to copy the program to...

Page 27: ...n at the same time or if they contain the same two stations but in reverse order as in the following two programs Program 1 Program 2 Station 1 Station 1 Station 2 Station 3 Station 3 Station 2 1 Select the program see steps 1 and 2 page 22 2 Press F2 Edit 3 Press F1 More 4 Press F2 Check 5 Using the and keys select the program number that you wish to check compatibility with 6 Press F2 Check The ...

Page 28: ... the program selected at step 1 Many of the program incompatibility situations arise from al location of water wash stations Therefore these stations are user selectable Appendix 3 gives some examples of common staining protocols which are compatible 5 Operation ...

Page 29: ...ime for the rack to be lowered into a station 5 6 Oven You can set the temperature of the oven in the range 30 65 C or select heating to be OFF The oven will operate at the setting selected during the en tire staining process whether or not is being used The se lected temperature will be displayed during staining To set view or change the oven setting 1 Press F3 SetUp from the Main Menu The curren...

Page 30: ...e displayed on the second line To alter the setting 2 Press F2 Dips 3 Press F1 to turn the dips on or Press F2 to turn the dips off or Use the and keys to alter the number of dips 4 Press F4 to return to the SetUp screen The new Dips setting will now be displayed 5 Press F4 to return to the Main Menu The time for a single Dip down and up is selected in Rack movement times Use this as a guide when ...

Page 31: ... rack entry time Down 3 To change any of the values press F1 Dip F2 Up or F3 Down to position the cursor beneath the appropriate value 4 Use the and keys to alter the setting 5 Repeat steps 3 and 4 as required 6 Press F4 Return to return to the SetUp screen 7 Press F4 Return to return to the Main Menu 5 9 Staining This section provides a guide to staining slides TheAutoStainerXLcanacceptslideracks...

Page 32: ... The Load and Exit drawer containers can be filled with a reagent if desired However the instrument will not control the immersion time in these sta tions 5 9 2 Wash system The wash system consists of five wash stations each capable of holding one slide rack Water enters the wash station from the base and exits from the overflow lip at the top left hand edge Wash stations have a locating pin and c...

Page 33: ...Menu The instrument will take a few seconds to initialize If a rack is already loaded then the Abort Menu will be dis played Press F1 Stain to continue 2 Select the required program number using the and keys Check to see that the load drawer is empty the Load LED will be lit Open the drawer and insert the slide rack ensuring that it is correctly seated Close the drawer 3 Press the Load key If the ...

Page 34: ...trument will be unable to finish the processing of further racks which require this station 5 9 6 Unloading racks from other stations If the final step in a program is not the exit drawer the Unload LED on the control panel will flash when processing is complete To unload the rack 1 Press F1 Unload A confirmatory message will be given while the head completes its cur rent operation The station num...

Page 35: ...heck change reagents abort staining of one more racks To interrupt staining 1 Press F4 Pause to return to the Abort screen If staining is interrupted immersion times during Pause will not be identical to those in the chosen program s If no racks are loaded then the Main Menu will be displayed 2 To abort a rack refer to page 35 or 3 Press F1 Stain to continue staining or 4 Press F4 Main Menu to ret...

Page 36: ...F2 Abort rack 3 Using the and keys select the station containing the rack you wish to abort 4 Press F2 Abort 5 Remove the rack as instructed Press F1 Done 6 To abort other racks repeat steps 3 to 5 7 Press F4 Cancel to exit from the Abort screen 8 Press F1 to continue staining or press F4 to return to the Main Menu as desired 5 Operation ...

Page 37: ...iods and flush well with water after use Exterior painted surfaces can be cleaned with a mild detergent and wiped with a damp cloth Avoid the use of solvents on exterior surfaces and especially on the control panel and lid Wipe the control panel carefully with a damp cloth 6 1 1 Wash containers Remove the wash containers and clean with detergent 6 1 2 Reagent containers Wash in warm water with det...

Page 38: ...ing the instrument will attempt to correct the problem first If it is unsuccessful then a message will be given and the instrument will wait for the user to rectify the problem Some faults cause the alarm to sound The alarm can be turned off by press ing F1 Quiet F2 Pause can be used to pause staining from the error message dis play A list of instrument messages and their meanings follows ...

Page 39: ... Slide rack bent The instrument will attempt to restart staining once the problem is rectified Even after attempting to restart staining the head is still unable to move freely Remove any obstructions and recommence staining or contact your service agent if the problem persists The outlet duct at the rear of the instrument is blocked Remove the blockage The oven has failed and must be serviced The...

Page 40: ... the drawer Ensure the Exit drawer is empty and close the drawer Program x is either empty or consists entirely of blank or zero time steps Program x is incompatible with a program assigned to a rack s currently being stained The rack s must be completed before program x can be used Refer to Page 26 The load drawer must be closed before the instrument can pick up the rack The Exit drawer must be c...

Page 41: ...ng and cannot be altered The message occurs during a compatibility check of two programs The stations specified are in the opposite order in the two programs which cannot be used concurrently Exit occurs before the end of the program and the remainder of the steps will be ignored A program which is currently being used for staining cannot be altered Copy the program to another program number and t...

Page 42: ... extend some station times Programs and SetUp have been lost and must be entered again The internal memory must be replaced Contact your service agent Increasing the amount of agitation while racks are currently being stained might extend exact immersion times Selecting continuous agitation will result in only one rack being progressed at a time 7 Trouble shooting ...

Page 43: ...iza tion from which you have purchased the contractual product shall apply exclusively Technical service information If you require technical service or replacement parts please contact your Leica sales representative or dealer who sold the product Please provide the following information Model name and serial number of the instrument Location of the instrument and name of the person to contact Re...

Page 44: ...awer 1 1 exit drawer 0 1 Programs 15 N 15 Steps per program 25 N 25 Note some steps can be blank Immersion time N A Y 0 sec 59 min 99 sec Note 0 seconds means the step is omitted Timing accuracy Exact N A Y 1 second Exact 0 infinity not Exact Oven temperature N A Y Off 30 65 C Agitation Dips N A Y Off 1 20 Continuous Agitation time Dip seconds cycle 2 Y 1 4 Rack withdrawal time Up seconds 9 Y 4 9 ...

Page 45: ... for wash station O ring hook O ring for wash container Drain hose Chimney adapter Inlet hose Reagent container handle Wash containers Slide rack 30 1 unit Plastic slide rack 30 1 unit Output rack Reagent container holder Lid for reagent containers inside the instrument Slotted lid for reagent container Program pad Instructions for Use To prevent damages to the instrument and specimens only those ...

Page 46: ...s 8 12 3 00 Y 7 4 00 Y Wash Wash 4 13 3 00 N 8 2 00 N 95 Alcohol 9 9 1 30 N OG 6 10 10 2 00 Y 95 Alcohol 11 11 1 30 N 95 Alcohol 12 12 1 30 N EA 50 13 13 2 30 Y Eosin 14 14 2 00 Y 95 Alcohol 15 15 0 30 y 14 1 30 Y 100 Alcohol 16 16 2 00 N 15 1 30 Y 100 Alcohol 17 17 2 00 N 16 1 30 Y 100 Alcohol 18 18 2 00 N 17 1 30 y Xylene Exit 19 18 Washes 1 to 4 and the stations between are used in the same seq...

Page 47: ...lkohol 3 4 2 00 N 100 Alkohol 4 5 2 00 N 70 Alcohol 5 6 1 00 N Wash Wash 1 7 2 00 N Hematoxylin 6 8 5 00 Y 1 5 00 Y Wash Wash 2 9 2 00 N 2 2 00 N Acid Alcohol 7 10 0 02 Y 3 0 02 Y Wash Wash 3 11 3 00 N 4 3 00 N Scott s 8 12 3 00 Y 5 3 00 Y Wash Wash 4 13 3 00 N 6 3 00 N Eosin 14 14 2 00 Y 95 Alcohol 15 15 0 30 Y 100 Alkohol 16 16 2 00 N 7 2 00 N 100 Alkohol 17 17 2 00 N 8 2 00 N 100 Alkohol 18 18 ...

Page 48: ...E RACK after PUTDOWN or DIPPING The process by which the HEAD attaches itself to the SLIDE RACK prior to PICKUP The IMMERSION time is achieved within 1 second Drawer into which SLIDE RACKS are placed by the instrument for subse quent collection by the user A fan draws fumes through a filter which removes dangerous SOLVENT va pors XYZ device used to PICKUP PUTDOWN ENGAGE DISENGAGE DIP and move SLID...

Page 49: ...o them Personal computer based on the original IBM architecture The SLIDE RACK is withdrawn from a STATION by the HEAD in such a way as to minimize CARRYOVER Series of STEPS by which a SLIDE RACK undergoes STAINING in the instru ment The SLIDE RACK is placed in a STATION by the HEAD Chemical used for STAINING Container holding REAGENT into which SLIDE RACKS are placed by the in strument Parameters...

Page 50: ...AINING sequence See HEAD The user removes a SLIDE RACK from the EXIT DRAWER or from the station in which it completes its programmed sequence Uninterruptable power supply which allows STAINING to continue during brief mains power failures Container through which water flows to wash REAGENT from a SLIDE RACK and the SLIDES in it STATION STEP TRANSFER ARM UNLOAD UPS BATTERY BACKUP WASH STATION Gloss...

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