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SVM340 Synchronized Video Microscope

User’s Guide

Summary of Contents for SVM340

Page 1: ...SVM340 Synchronized Video Microscope User s Guide...

Page 2: ...uScope software 2009 2018 LabSmith This manual 2018 LabSmith No part of this document may be reproduced without the written consent of LabSmith...

Page 3: ...roscope stage 14 3 uSCOPE SOFTWARE 16 3 1 X Y Traverse 17 3 2 Focus 17 3 3 Illumination 19 3 4 Presets 20 3 5 uScope Instrument and Video File Settings 20 3 6 Online and offline operation 20 3 7 Upgra...

Page 4: ...frames 35 5 9 Distance indicator 35 5 10 Video Zoom and Pan 37 5 11 Hot Pixel Suppression 37 6 PROBES 38 6 1 Velocity PIV probes 38 6 2 Polygon and Macropixel intensity probes 42 6 3 Particle Counting...

Page 5: ...r In addition the SVM340 includes an advanced programmable synchronization unit with four inputs and three outputs for synchronizing image acquisition to external events The basic functions can be con...

Page 6: ...disk as avi files and perform advanced real time video processing 1 3 Computer requirements The uScope software is designed to stream digitized video sequences directly to disk so it is recommended th...

Page 7: ...on driver Cam33U_setup exe which is included on the SVM installation disk If your SVM340 includes a DP1 Optics Module A DP1 BW A DP1 C or A DP1 EPI you will install a Sentech installation driver Two d...

Page 8: ...ur threaded holes on the chassis bottom c Adjust each foot as necessary to eliminate any rocking of the chassis Installed standoff Note 2 50 mm standoffs are required for the DP5 Optics Modules Contac...

Page 9: ...nm White n a Standard Illuminator Modules Name Channel A Channel B Channel C Channel D LED B Blue Blue White Blue LED G Green Green White Green LED R Red Red White Red LED Y Yellow Yellow White Yellow...

Page 10: ...de and gently pulling the module down from below the SVM340 body until it comes free Important Turn off power to the SVM340 before removing or inserting an optics module Optics module The Digital Opti...

Page 11: ...objective is not installed Removal or changing of the objective should be done in a clean environment when possible Cover the objective opening i e with a piece of tape if the optics module will be s...

Page 12: ...D represent four different stored positions and the keypad controls the traverse movements The focus buttons move the objective up and down for focusing When the LIGHT button is lit buttons A D repre...

Page 13: ...our buttons will move the traverse in the x and y directions Pressing a button will start the traverse motor at low speed and after about two seconds motor speed slowly ramp up to high speed Pressing...

Page 14: ...alog Optics Modules Video Output for the Digital Optics Module DP1 or DP5 is via the USB cable connected directly to the front or bottom of the Optics Module Digital Inputs and Outputs The four digita...

Page 15: ...e signals to drive 4 external illumination sources with settings similar to the four bank led module Pin connections 1 5 V DC max 3 A 2 Chassis Ground 0 V 3 Chassis Ground 4 Chassis Ground 5 Chassis G...

Page 16: ...a polished stainless steel plate which can be machined to provide application specific mounts for the fluidic device electrodes fluid hoses or other fittings Removing the microscope stage LabSmith al...

Page 17: ...o four screws installed on the Delrin stage The inner stage mounting holes are used for the LS600 CH the outer mounting holes are used for the iBB Integrating Delrin stage shown assembled with LS600 C...

Page 18: ...ntil you succeed in communicating with the SVM or you click Cancel then you work offline and uScope does not try to send commands to the SVM Communications settings dialog box Some functions are avail...

Page 19: ...lications it may be useful to temporarily reduce the motion limits of the x y or focus The motion range can be reduced by selecting SVM Motion Limits from the main menu Modification of these limits ma...

Page 20: ...pixels Blurs image to reduce the effect of image noise on focus algorithm May need to be increased for noisier images Final passes default 1 Sets number of passes to make once focus location is establ...

Page 21: ...ED Pulse Durations dialog box When an EPI optics module is installed the forth slider controls the EPI illumination and channel D is controlled with the Channel B slider button The maximum time durati...

Page 22: ...uScope Instrument and Video File Settings The uScope software saves the instrument and video settings in a file with usc extension To open an uScope file choose File Open then locate the file on your...

Page 23: ...the uScope main window 6 When the progress indicator reaches 100 the upgrade is completed Important Do not turn off or disconnect the SVM340 or the PC while the upgrade is in progress This may result...

Page 24: ...indow to Open a Saved Video will pop up as shown below If a live image was viewed before uScope was closed uScope will automatically try to connect to the same Optics Module If that Optics Module is n...

Page 25: ...DP1 C and DP1 EPI optics modules will be listed under the module name or as StStream Capture Note If your video capture device is not listed in the Detected Video Capture Hardware Window ensure that t...

Page 26: ...through both the SVM340 and the USB cable Turn off the SVM340 and unplug the USB cable when not in use to save power and reduce heat generation in the optics module 4 2 Adjusting the Image Output Siz...

Page 27: ...change the output size go to Video Image size and format Note The DP5 Optics Modules must be connected to a USB3 0 or USB3 1 port to use the full image size Frame Rate The maximum frame rate will dep...

Page 28: ...ure consistent readings Gain This property manipulates a factor that is used to multiply every pixel value Recommend setting start low and adjust up as needed Gain can also be set from the Video Proc...

Page 29: ...applicable for SVM340 operation 4 4 Color Format set up The SVM340 can be fitted with either a grayscale B W or color camera module The uScope software provides many options for viewing and recording...

Page 30: ...later be viewed by Windows Media Player or other video playback software off line processed by uScope or other video processing software compatible with the AVI standard To enable uncompressed video...

Page 31: ...e display Buffering also allows the user to selectively record events after they occur For instance to catch a particular particle or droplet passing through the field of view the user can set uScope...

Page 32: ...buffer is normally larger than the RAM buffer 5 4 Recording a video To record a video set the time in the Record from box to the desired number of seconds or drag the slider bar between now and the ma...

Page 33: ...d The Snap Shot Settings Dialog is opened from View Snap Shot Settings Snap Shot Settings Dialog box Averaging If you have a little to no motion increasing the number of frames or time will improve yo...

Page 34: ...Image minimum is set to black maximum set to white Manual Set minimums black and maximum white to desired level Flash Settings This function is useful to prevent photo bleaching or heating of your sam...

Page 35: ...before it takes the photo Image File naming This option allows you to set the default for the type of file compressed jpg or uncompressed bmp and the naming The files can be auto named or you can cho...

Page 36: ...will reduce the visual appearance of the blurring caused by the interlacing by various algorithms The deinterlace method is selected in the Video Deinterlace Video options dialog box The best deinter...

Page 37: ...software uses a buffer as it is recording to minimize dropped frames However if the available RAM isn t sufficient you may still experience dropped frames Improving available RAM Set the RAM buffer s...

Page 38: ...ation is based on the magnification setting for the objective If the magnification setting is incorrect the distance will be incorrect If you are evaluating a saved image you will need to know what ma...

Page 39: ...o zoom out to full image size Zoom in Click to select Use mouse to draw image area Pan Click and drag mouse to pan image only applicable for zoomed image 5 11 Hot Pixel Suppression Hot pixel suppressi...

Page 40: ...r can handle The three types of probes that can be made in uScope are the Velocity PIV probes Intensity probes and Particle Counting Probes 6 1 Velocity PIV probes uScope software makes it easy to cre...

Page 41: ...be A probe such as the one below will appear New probe showing correlation field vector arrow and real time velocity as text 3 Repeat step 2 to create as many probes as desired 4 Once created the prob...

Page 42: ...ow the area over which statistics will be calculated Some guidelines for setting the probe window size are a Probes should typically be the same size in both x and y directions b Smaller probes requir...

Page 43: ...for every third frame etc This option is useful for examining very slow flows b Subtract Mean subtracts the background nonmoving data from the flow calculations The amount of data that will be subtra...

Page 44: ...Intensity or polygon probes are used to track the color spectrum or fluorescence intensity inside a defined region The advantages of the polygon probe are that 1 you can make the probe an arbitrary s...

Page 45: ...can be selected by selecting the probe left click then right click and select Properties The following dialog box will appear Polygon Probe Properties dialog box For images from the B W or EPI optics...

Page 46: ...ight click on the probe and select Properties brings up the following window Macropixel Probe Properties Changing the macropixel size changes the number of pixels per sub probe The probe data can also...

Page 47: ...e function 2 Double click on region of image Use handles to size the rectangle as desired 3 Right click and select properties to adjust thresholds and set particle filters Particle Probe Property Sett...

Page 48: ...ing un evenness or out of focus background in an image For best results set the start threshold equal to or greater than the stop threshold Adjust the thresholds up or down until only real particles a...

Page 49: ...recording Check Append the date Append the time and or Append counter to add these values to the new file names An example of how the name will appear is shown at the bottom of the dialog box Note Yo...

Page 50: ...depend on the display options selected in the Polygon Probe Properties window Macropixel probe data The macropixel probe data contains four columns for each probe Macropixel sum from X Y defines the...

Page 51: ...alog Optics Modules only go to Video Physical Input Connector and select S video for the input Frame rate not set Digital Optics Modules only go to Video Frame Rate and select a frame rate optics modu...

Page 52: ...itation emission for the fluorescent dye used Problem SVM can t focus on target Possible Cause Resolution target above maximum focus range The top focus range of the SVM is limited to prevent interfer...

Page 53: ...nd choose the correct source Shut down the uScope software turn off the SVM and then restart the SVM and software and select the correct source For Analog Optics Module If the errors persist go to Vid...

Page 54: ...cal Aperture 0 25 Working Distance 6 3 Focal Length 16 76 20X Numerical Aperture 0 40 Working Distance 3 3 Focal Length 8 55 spring loaded Illuminator modules LED B 3 blue 460 nm bandwidth 50 nm one w...

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