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Confocal Laser Scanning

Biological Microscope

FV1000

FLUOVIEW

FLUOVIEW—Always Evolving

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Summary of Contents for Fluoview FV1000

Page 1: ...Confocal Laser Scanning Biological Microscope FV1000 FLUOVIEW FLUOVIEW Always Evolving...

Page 2: ...cated laser light stimulation scanner to achieve simultaneous targeted laser stimulation and imaging for real time visualization of rapid cell responses The FV1000 also measures diffusion coefficients...

Page 3: ...2 Imaging to Analysis ing up New Worlds From Imaging to Analysis FV1000 Advanced Deeper Imaging with High Resolution FV1000MPE...

Page 4: ...se optical advances boost the overall system capabilities and raise performance to a new level Imaging Stimulation and Measurement Advanced Analytical Methods for Quantification Now equipped to measur...

Page 5: ...4...

Page 6: ...with Minimum Phototox Two versions available Single fiber type combiner is used for main scanner FV1000 with up to six lasers ranging from 405 to 635 nm Dual fiber type combiner is used for laser ligh...

Page 7: ...Tissue culture dishes slide chambers microplates and glass slides can be used with live cells and fixed specimens BX61 UIS2 Objectives Olympus UIS2 objectives offer world leading infinity corrected o...

Page 8: ...ength detection range 495 nm 561 nm in 2 nm steps Excitation wavelength 488 nm Courtesy of Dr Shigeo Okabe Department of Anatomy and Cell Biology Tokyo Medical and Dental University EGFP EYFP Fluoresc...

Page 9: ...and circles SIM Scanner Unit for Simultaneous Laser Light Stimulation and Imaging Branching of laser in laser combiner Lasers are used for both imaging and laser light stimulation LD405 LD635 AOTF AOT...

Page 10: ...in in Ptk2 cells labeled with two colors 405 nm 635 nm and compared Improved Flatness and Resolution at 405 nm Better flatness reduces the number of images for tiling 450 400 0 5 0 0 Focal plane m 0 5...

Page 11: ...ide field of view with improved transmittance at near infrared wavelengths A correction collar compensates for spherical aberration caused by differences between the refractive indices of water and sp...

Page 12: ...al available scanning modes including ROI point and high speed bidirectional scanning Configurable Excitation Laser Power Easily adjust the optimum laser power for each specimen live cells and fixed s...

Page 13: ...ables multiple measurement points in glass slides 35 mm dishes or individual microplate wells Repeated imaging of multiple cells improves the statistical power of time lapse experiments Mosaic Imaging...

Page 14: ...ad Application Support and Sophisticated Experiment Control Measurement Light Stimulation Multi Dimensional Time Lapse FRET Colocalization 3D Mosaic Imaging Multi Color Imaging 3D 4D Volume Rendering...

Page 15: ...s and proteins 3D 4D Volume Rendering One click 3D 4D image construction from acquired XYZ T images Change the angle of 3D image with a single click Colocalization Configurable threshold values for fl...

Page 16: ...fied regions Cross correlation RICS ccRICS characterizes molecular interactions using fluorescent labeled molecules in two colors FRAP Analysis The Axelrod analytical algorithm is installed as a FRAP...

Page 17: ...btained for each pixel was sampled at different times so the data for each pixel is affected by the passage of time in addition to its spatial XY information By analyzing this image data with a new st...

Page 18: ...ages it is possible to characterize the diffusion speed of the molecule and the speed of binding and release between the molecule and cell structures Example Fluorescence recovery without interactions...

Page 19: ...on Dynamics Before Stimulation After Stimulation 405 nm 405 nm Caged Glutamate Fluorescent calcium indicator Fluo 3 in HeLa cells Image acquisition at 1 second intervals Using the caged compound Bhcmo...

Page 20: ...the environment inside the tissue culture dish completely stable The environment is precisely maintained at 37 C with 90 humidity and 5 CO2 concentration ZDC Baseline focal plane IR Laser for focal pl...

Page 21: ...asily register wide areas and the thumbnail display provides a view of the entire image acquired during the mosaic imaging process Coordinate Information Thumbnail CNS markers in normal mice Objective...

Page 22: ...ic imaging to form a composite image Acquisition of adjacent Z series images for 3D mosaic imaging Software and Equipment Required Multipoint time lapse software motorized XY stage TIRFM TIRFM imaging...

Page 23: ...dability 350 350 300 300 400 400 450 450 500 500 550 550 600 600 650 650 700 700 750 750 800 800 405 LD 440 LD 473 LD 635 LD 559 LD 458 M ulti Argon 515 M ulti Argon 543 G reen H eN e 488 Argon mCherr...

Page 24: ...light path ensures that expandability of the microscope itself is not limited Visible UV and IR laser introduction ports are provided as well as a feedback control system Illumination Units Convention...

Page 25: ...CO2 incubator Motorized XY stage Scanning unit for IX81 Spectral type or Filter type detector system 559 nm 458 488 515 nm 405 nm A B D E F E F G D B E A F G A B D G C FV1000 System Diagram IX81 ZDC...

Page 26: ...line with free orientation free line Point scanning Line scanning Straight line with free orientation free line Point scanning Photo Detection Method 2 detection modes Analog integration and hybrid ph...

Page 27: ...ciences Cerebellum Purkinje cell Courtesy of Dr Tetsuro Kashiwabara Assistant Professor and Dr Akira Mizoguchi Professor Neuroregenerative medicine course Mie University School of Medicine Drosophila...

Page 28: ...isit our web site for details Windows is a registered trademark of Microsoft Corporation in the United States and other countries All other company and product names are registered trademarks and or t...

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