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Problem: very dim video image
Possible Cause
Resolution
imaging settings
adjustment
For Analog Optics Modules:
•
adjust the
Brightness
or
Contrast
settings in
Video>Brightness/Contrast/Hue/etc...> VideoProc Amp
For Digital Optics Modules:
•
adjust the
Gain
setting in
Video>Brightness/Contrast/Hue/etc...>Property
•
adjust the
Brightness
,
Contrast
,
Sharpness
or
Gamma
settings in
Video> Brightness/Contrast/Hue/etc...> VideoProc Amp
incorrect excitation or
filter for fluorescent
imaging
For standard B&W optics module (digital or analog)
•
ensure the illuminator wavelength is the correct excitation for
the fluorescent dye being used
•
if a filter is installed (under the objective) ensure its wavelength
range is correct for the fluorescent emission
For EPI optics module (digital or analog)
•
ensure the EPI optics module has the correct excitation/emission
for the fluorescent dye used
Problem:
SVM can’t focus on target
Possible Cause
Resolution
target above
maximum focus range
The top focus range of the SVM is limited to prevent interference
between the illuminator module and the stainless steel stage. If the
target can’t be brought within the focus range one option is to use
an objective spacer to increase the focus height by 1.25mm. This
solution works best when using an objective with a long working
distance (i.e. 4X) and without the 4-channel illuminator (i.e. with
the EPI Optics Module).
Problem: no EPI illumination
Possible Cause
Resolution
incorrect optics
module configuration
Select View>Camera Configuration and ensure Epifluorescence
Illumination Type is selected. NOTE: ensure you have an EPI optics
module installed before you change the configuration.
EPI illuminator turned
off
EPI illuminator adjustment replaces the Channel D adjustment
slider/button when an EPI module is installed (Channel D
illumination control is ganged with channel B)
