6
6. Allow the solution to cool to 60
o
C. Place the flask in a hood and
add 5 ml of 10X MAE buffer (see Appendix A for recipe), and 1.5
ml of 37 % formaldehyde.
CAUTION:
Formaldehyde vapors are toxic. Gel
preparation should take place in a hood and solutions and gels
containing formaldehyde should be kept covered when
possible.
Proceed with Section C, Step 1, “Casting the Gel” on page 12.
C.
Casting the Gel
1. Place the gel casting stand on a lab bench.
CAUTION!
Cast agarose gels containing formaldehyde in a hood.
2. Insert the gel casting tray into the casting Stand. If you are using
the 12 x 6 cm gels place the spacer in the center of the casting
Stand, then insert the two 12 x 6 cm landscape gel trays (see
direction 2 below).
3. When the gel solution has cooled to approximately 55
o
C, slowly
pour it into the gel tray. If hotter gel solutions are routinely poured
the tray may warp over time.
4. If bubbles form on the surface of the gel upon pouring, use the
Summary of Contents for ENDURO GEL XL E0160
Page 18: ...NOTES...
