12
Solutions containing MOPS should be wrapped in aluminum foil and stored
at room temperature. The buffer tends to yellow with age. Light yellow
buffer may be used, however, dark yellow solutions should be discarded.
Sample Loading Buffer, DNA
Sample Loading Buffer, RNA
10X Stock Solution:
5X Stock Solution:
50 % Glycerol
1 mM EDTA, pH 8.0
100mM Na3EDTA
0.25 % Bromophenol Blue
1% SDS
0.25 % Xylene Cyanol
0.1% Bromophenol blue
50 % Glycerol
pH 8.0
B.
Physical Properties of Electrophoretic Plastics
Figure A: UV Transmission Characteristics of UV Gel Tray
The UV transmittant tray is ideal for monitoring the progress of electrophoresis without removing
the gel from the tray. Figure A above clearly delineates the absorption specifications of the UV
transmittant plastic gel tray. Minimal transmission is seen below
V.
REFERENCES
1.
Lehrach, H., et al. 1977.
Biochemistry
16:
4743.
2.
Sambrook, J., Fritsch, E.F., and Maniatis, T., (1989). Molecular Cloning, A Laboratory
Manual, vol 1. Cold Spring Harbor Press, New York.
3.
Selden, R.F. (1988) Analysis of RNA by Northern Hybridization,” in
Current Protocols in
Molecular Biology
, F.M. Ausubel, et. al, editors, volume 1, p.4.9.1. Green Publishing
Associates and Wiley-Interscience.
Technical Support and Information Services:
Our
staff is available to advise you with any questions regarding our products or
their specific application.
Wavelength, nm
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
2
7
5
2
8
7
3
0
0
3
1
2
3
2
5
3
3
7
3
5
0
3
6
2
Summary of Contents for ENDURO GEL XL E0160
Page 18: ...NOTES...
