1
I.
MAINTENANCE
Please handle the unit with care:
Do not
expose the unit or its accessories to temperatures above
60°C.
Do not
expose the unit to organic solvents.
Do not
clean the unit with abrasive cleaners or cleaning aids.
In most cases, rinsing with deionized water will sufficiently clean the unit.
For heavier dirt, use a mild cleansing solution such as dish soap (alkaline
cleansers are not recommended). Hand wash and dry with a soft cloth.
To remove residual ethidium bromide, occasionally soak the unit in 1%
commercial bleach solution for 16 hours. Rinse well.
PLEASE NOTE
: The degradation of acrylic due to solvents may result in
substantial discoloration, cracking, warpage, or etching of the
electrophoresis unit.
Do not
apply any of the following solvents: benzene, xylene,
toluene, chloroform, carbon tetrachloride, alcohols, phenols, ketones,
or esters.
Do not
expose the ABS combs supplied with this unit to
formaldehyde for extended periods. When casting gels containing
formaldehyde, remove the combs promptly upon hardening of the gel
and rinse completely with deionized water.
Elimination of RNase Contamination
Should treatment of the unit to eliminate RNase contamination be desired, clean
the unit with a mild detergent as described above followed by soaking for 10
minutes in a solution of 3 % hydrogen peroxide, and then for 1 hour in 0.1 % DEPC
(diethyl pyrocarbonate). Pour out final rinse, and air dry.
CAUTION:
DEPC is a suspected carcinogen; handle with care.
Alternatively, soak the unit and accessories in freshly made 2.2 mM acetic
anhydride treated water (200 µl/liter) for at least five minutes. Solutions for RNA
work (electrophoresis buffers, etc) may be made from the same acetic anhydride
treated water as well.
Summary of Contents for ENDURO GEL XL E0160
Page 18: ...NOTES...