Labnet ENDURO GEL XL E0160, User Manual

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3
Power Supply
Overall dimension 7.5 × 17.0 × 6.2 cm
Weight 410 g
Input Voltage AC100 - 240V, 50/60Hz
Output Voltage 10 to 150 volts; Constant peak
voltage of 150V
Output Amperage 10 to 400 mA
Maximum Wattage 45 W
Timer 99 hours 59 min, and continuous
model
Safety Switch Micro-sensor (hall) in the Power
Supply. No output without safety lid,
Memory Function Automatic memory (the last used V
and T)
III. OPERATING INSTRUCTIONS
A. Preparation of the Agarose Gel and Electrophoresis Buffer -
DNA
1. Select the percentage gel necessary to effectively resolve your
sample, using Table 1 as a guideline.
Table 1: Gel Concentrations and Resolving Ranges
Table taken from Sambrook, J., Fritsch, E.F., & Maniatis, T. (1989)
Molecular Cloning, A Laboratory Manual, 1, 6.8 613.
2. Weigh an appropriate quantity of agarose (0.3 % means 0.3 g of
agarose per 100 ml of gel volume) and place it into a 250 ml flask.
Note a 4mm gel will use 100 mls of agarose solution.
3. Make 500 ml of either 1X TAE or 1X TBE electrophoresis buffer
(see below).
Electrophoresis Buffers
The two most commonly used buffers for horizontal
electrophoresis of double stranded DNA in agarose gels are Tris-
Acetate-EDTA (TAE) and Tris-Borate-EDTA (TBE). While the
Concentration of Efficient Range of
Agarose in Gel Separation of Linear DNA
(% w/V) (Kb)
0.3% 5 - 60
0.6% 1 - 20
0.7% 0.8 - 10
0.9% 0.5 - 7
1.2% 0.4 - 6
1.5% 0.2 - 3
2.0% 0.1 - 2