KNAUER
P 6.1L User Manual V6890 Version 1.2
35
Operation
Power plug
Make sure that the power plug on the rear of the device is
always accessible, so that the device can be disconnected from
the power supply.
Next steps
Finish the installation and connect the device to the mains.
Operation
In this chapter you find information which are relevant for oper-
ating the pump.
Note
Prior to switching on the pump, you should de-aerate it to
remove air from capillary and tubing.
Initial Start-Up
Use this checklist to determine whether the detector is ready for
the initial start-up:
Devices is positioned in the correct location.
The power plug has been pulled.
If the device is part of an HPLC system, you should also note the
following:
The network connection to the router is established.
The chromatography software has been installed by KNAUER
or a company authorized by KNAUER.
The capillary has been connected.
Pump Head
To bring a newly installed pump head into service securely, it
should run in prior to its first operation. For this purpose, set the
following parameters:
Back pressures higher than 8 MPa are generated by back pres-
sure regulators.
De-Aerating the Pump
Before the pump can be used, it must be de-aerated.
Mode “Flow on”
The pump can only be de-aerated when switched on because
the syringe can only take in liquid in "Flow on" mode. Only then
are the valves of a binary pump opened.
Pump head 5 ml, 10 ml
Pump head 50 ml
Solvent
H
2
O (degassed) or ethanol
H
2
O (degassed) or ethanol
Back pressure
30 MPa (min > 15 MPa)
12 MPa (min > 8 MPa)
Flow rate
2–5 ml/min
5–20 ml/min
Run time
30 min
90 min
Drag
Capillary with inner diameter
of 0.1 mm
length 2 m
Capillary with inner diameter
of 0.1 mm
length 1 m
