Knauer Axura DAD 2.1L User Manual Download Page 59

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The information in this document is subject to
change without prior notice.

Original edition of this manual, version 3.2

Last update: 2016/10/13

Printed in Germany on environmentally friendly
paper from sustainable forests.

® AZURA is a registered trademark of

KNAUER Wissenschaftliche Geräte GmbH

www.knauer.net/downloads

KNAUER
Wissenschaftliche Geräte GmbH
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14163 Berlin

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Telefax:

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Internet: www.knauer.net

HPLC · SMB · Osmometry

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Summary of Contents for Axura DAD 2.1L

Page 1: ...Detector DAD 6 1L DAD 2 1L MWD 2 1L User Manual V6700...

Page 2: ...ta 12 Transport 12 Operating Environment 13 Setup 13 Inserting the Flow Cell 14 Inserting the Flow Cell with Fiber Optics 14 Connecting the Capillaries 15 Using the PEEK Fittings 16 Connecting the Lea...

Page 3: ...onnection 30 Remote Operation Fiber Optics 30 Selecting the Wavelength 30 Bandwidth 31 Spectral Range 31 Time Constant Data Rate 31 Integration Time 32 Subtraction of the Baseline Chromatogram 33 Exte...

Page 4: ...on of Conformity 50 Warranty Seal 51 Disposal 51 Repeat Orders 51 Device and Accessories 51 Flow Cells 53 Flow Cells with Fiber Optics 53 Fiber Optic Cables 54 HPLC Glossary 55 Index 57 To whom it may...

Page 5: ...ice there are the flow cell and the removable lamp cover for lamp replacement Rear View On the back of the detector the connectors for LAN and power plug are located as well as the serial number of th...

Page 6: ...1L separate photodiodes the photodiode array The optical system of the DAD 6 1L is depicted in figure 4 An active tem perature control with 0 5 C sensitivity ensures baseline stability for minimal bas...

Page 7: ...te of 100 Hz During the measurement approx 100 spectra per second are recorded Leak Management Integrated leak management drains liquids from leaks GLP Data The Mobile Control the Control Unit and the...

Page 8: ...ents e g Fluorinert FC 75 FC 40 perfluorinated polyether e g Fomblin Scope of Delivery Note Only use original parts and accessories made by the manufacturer or a company authorized by the manufacturer...

Page 9: ...l spare parts tools and solvents made or recommended by KNAUER Good Laboratory Practice GLP Accident prevention regulations published by the accident insurance companies for laboratory work Filtration...

Page 10: ...dangers related to the device are divided into personal and material damage in this user manual Lethal injuries will occur Serious or moderate injuries can occur Minor injuries can occur Device defec...

Page 11: ...ce by carrying or lifting it on protruding housing parts The device may fall and thus cause injuries Lift the device on the side of the housing only Lifiting Clasp the device at its side panels and li...

Page 12: ...device accessible to be able to disconnect the device from the mains General requirements Position the device on a level and even surface Protect the device against direct exposure to sunlight Set up...

Page 13: ...t one used previously Other wise purge the flow cell with a medium miscible with both the eluents Prerequisites No flow cell or test cell is installed The device is switched off Note Pay attention to...

Page 14: ...and isopropanol Next Steps Connect the capillaries Connecting the Capillaries Capillaries connect the detector with the column and guide liquids For the flow cell connectors we recommend PEEK capillar...

Page 15: ...he device and the data acquisition via chromatogra phy software are stopped Prerequisite The front cover has been removed Process Process Figure 1 Push the capillary 1 through the fitting 2 2 Slide th...

Page 16: ...ctor is located on the side of the device 3 Connect the nozzle and the fun nel 4 Push the other end of the hose onto the nozzle 5 of the leak tray Fig 14 Hose connected to device 5 For the bottom devi...

Page 17: ...een saver must be deactived In case you use an USB to COM box the option Allow the computer to turn off ths device to save power in the devicemanager must be deacti vated for all USB hosts Only for Wi...

Page 18: ...a phy software can be used to remotely control the system Integrating the LAN into a Company Network A network administrator can integrate the LAN into your company network In this case you use the WA...

Page 19: ...Settings 2 On the General tab choose the device name 3 Under Network Settings choose the setting Static 1 4 Enter the IP address into the text box IP Address 2 5 If necessary change the subnet mask a...

Page 20: ...ng depends on the settings in the Control Unit Mobile Control or software Steady rate signal passive open relay contact active closed relay contact Pulse Closed relay contact for at least 1000 ms Perm...

Page 21: ...s Wear a protective bracelet against electrostatic discharge and ground Start IN TTL Input Low active Secure switching threshold at least 10 mA After receiving a signal short circuit to ground from an...

Page 22: ...to account for larger inrush currents when switching on the modules The maximum power input is 75 W Prerequisite The electrical power supply at the installation site must be connected directly to the...

Page 23: ...Pre installed stainless steel and PEEK capillaries are color coded according to their inner diameter Note PEEK capillaries are not suitable for use with pure acetonitrile Aceto nitrile can cause capi...

Page 24: ...ctor into service Operation There are several options for controlling the device with chromatography software with Control Unit with Mobile Control Note It is not possible to use 2 control methods sim...

Page 25: ...vice and app is actualized through wireless LAN You find a detailed description on the Mobile Control in its accompanying user man ual Meaning of the LEDs There are three LEDs and a switch on the fron...

Page 26: ...vibrations green The deuterium lamp is active Right LED green The device has been switched on flashes green The device not ready for operation Wait until the device is ready for operation blue The dev...

Page 27: ...d remote operation are further fac tors that need considering when selecting a flow cell Flow Cell Volume Depending on your instrument setup column and sample s one flow cell volume may be more approp...

Page 28: ...etectors A longer path length therefore increases the sensitivity of a method see figure 25 The limit of detection is inversely proportional to the path length Wetted Parts The flow cell materials mus...

Page 29: ...to the length of the fiber optic cables Temperature changes around the cables may cause additional drift Selecting the Wavelength Signal Wavelength Wavelength selection can influence the sensitivity s...

Page 30: ...include the signal wavelength and the reference wave length when applicable Time Constant Data Rate Response Time The time constant influences the response time of the detector The response time dete...

Page 31: ...large Some general considerations are listed below Each peak should be defined by 20 30 data points For chromatograms with co eluting peaks or low signal to noise ratios 40 50 data points per peak are...

Page 32: ...ed chromatogram with an ideally flat baseline Extended Linear Range Upon activating the extended linear range option it is possible to broaden the linear range of the AZURA detector through internal s...

Page 33: ...nality of the device Operation Qualification OQ The Operation Qualification includes an extensive functionality test accord ing to KNAUER standard OQ documents and must be purchased from the manufactu...

Page 34: ...l connections Are the patch cable connected to the LAN ports and not the WAN port Are all cable connections between devices and router correct Are the cables plugged in tightly 4 If the router is inte...

Page 35: ...work Cannot proceed D2 lamp heating Manual validation is not possible while lamp is heating up Wait until deuterium lamp has initial ized and continue afterwards Cannot proceed D2 lamp off Manual vali...

Page 36: ...system has not been validated Instrument remote controlled This entry is not executable Quit software L Lamp cover open The lamp cover was mounted incorrectly or the micro switch is malfunctioning Mou...

Page 37: ...r against voltages inside the device Leak was detected Switch off the device Remove the leak and start the device afterwards N No D2 lamp detected Check if the deuterium lamp was installed cor rectly...

Page 38: ...see Repeat Orders on page 51 Contacting the Technical Support If you have any technical questions regarding KNAUER hardware or soft ware please use one of the contact options below Technical Support h...

Page 39: ...ially available cleaning solution or with isopropanol Device defect Intruding liquids can cause damage to the device Place solvent bottles next to the device or in a solvent tray Moisten the cleaning...

Page 40: ...the baseline and reduced sensitivity can be a Increased noise of the baseline and reduced sensitivity can be a result of a dirty flow cell Often it is sufficient to rinse the flow cell to restore opti...

Page 41: ...ed the peristaltic pump is configured to pull through the cell to avoid possible contamination from degraded peristaltic pump tubing To lessen the time required for this cleaning method large bubbles...

Page 42: ...ht path like fiber optic ends become contami nated if being touched with the fingers Avoid touching the ends without gloves Use alcohol and a cotton bud for cleaning Next Steps Connect the capillaries...

Page 43: ...fe threatening risk Disconnect the power supply before change the lamps Check the status of the lamps in the software and on the LEDs Burns Burn hazard from hot lamp The lamp needs to cool down before...

Page 44: ...hands Wear gloves Use a clean soft cloth for cleaning Note You can clean the lamp thoroughly with a lint free cloth and isopro panol 3 Loosen the lock ring 3 or 4 of the lamp plug and pull out the plu...

Page 45: ...alling the deuterium lamp 6 Attach the lamp cover and fix it with the screws 8 and 9 Fig 38 lamp cover Process Process Figure 7 6 4 5 8 9 Process Process 1 Remove the leak 2 Dry the leak tray 3 Acknow...

Page 46: ...digital LAN remote connector manual Mobile Control or Control Unit optional programming wake up IP protection class IP 20 Technical Parameters GLP function Detailed report about lamp recognition opera...

Page 47: ...z analog inputs Error IN Start IN Autozero 0 10 V Analog IN outputs Events 1 2 Error OUT 5 V Valve 24 V Valve OUT analog outputs 4 0 5 V 16 bit control digital LAN remote connector manual Mobile Contr...

Page 48: ...0 s integration time automatic 5 1000 ms Communication maximum data rate 100 Hz LAN 12 5 Hz analog inputs Error IN Start IN Autozero 0 10 V Analog IN outputs Events 1 2 Error OUT 5 V Valve 24 V Valve...

Page 49: ...m pany within three workdays Warranty Conditions The factory warranty for the device is stipulated by contract During the warranty period any components with material or design related defects will be...

Page 50: ...efined by the WEEE directive End users can send their old devices man ufactured by KNAUER back to the distributor the importer or the company free of charge but would be charged for the disposal Solve...

Page 51: ...Kit kit LightGuide flow cells 1 16 A9842 kit UV flow cells 1 16 A9843 kit UV flow cells 1 8 A9844 Mounting Bracket mounting bracket for flow cells A9853 5 Mobile Control Mobile Control license with 10...

Page 52: ...ureProof Flow Cell Cartridge path length connection volume wetted parts max flow rate max pressure 3 mm 1 16 2 l titanium quartz PEEK 50 ml min 300 bar AMB18 Technical Data Order Number Technical Data...

Page 53: ...d parts max flow rate max pressure 0 5 1 25 2 mm 1 8 1 7 4 3 6 8 l PEEK quartz PTFE 1000 ml min 100 bar A4079 path length connection volume wetted parts max flow rate max pressure 0 5 1 25 2 mm 1 4 1...

Page 54: ...se through which mobile phase flows result ing in a chromatographic separation correction factor factor that arithmetically corrects device related deviations from measur ing values dead volume Dead v...

Page 55: ...loop A loop which is separate from a chromatographic system and which the sample is injected to After a switch is actuated the solvent flow passes the loop and the sample is flushed onto the column so...

Page 56: ...ata rate 8 31 32 default 32 optimize 32 dead volume 30 decommissioning 40 decontamination 11 default settings 27 degasser 55 detector 55 optimize 27 rear view 6 technical data 46 device overview 6 dis...

Page 57: ...LAN 19 power connect 23 disconnect 13 plug 13 power cable 10 power strip 11 power supply 11 pressure maximum 29 problems 35 further measures 36 professional group 10 R rear view 6 reference wavelengt...

Page 58: ...6700 version 3 2 Index 59 Technical Support 39 test Installation Qualification 34 Operation Qualification 34 time constant 31 transport damage 50 troubleshooting 35 LAN 35 U USB 7 W warm up time 28 wa...

Page 59: ...Printed in Germany on environmentally friendly paper from sustainable forests AZURA is a registered trademark of KNAUER Wissenschaftliche Ger te GmbH www knauer net downloads KNAUER Wissenschaftliche...

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