Izon qNano, User Manual, Page: 38 / 56

NANOPORE SET-UP AND SYSTEM OPTIMISATION
Figure 13.Schematic of the fluid cell assembly and key parts for using the qNano system.
4 /
Fluid Cell Cap
Upper Fluid Cell
Lower Fluid Cell
Order of operation:
1 / Prepare the fluid cell and stretch the nanopore
2 / Wet the nanopore
3 / Establish a stable baseline current
4 / Estimate the pore size via a conductance check (change pore if necessary)
5 / Coat the pore (if working with biological samples)
6 / Optimise signal-to-noise ratio for calibration particles
7 / Adjust conditions for the sample if necessary and record data
8 / Re-run calibration measurements to ensure system stability
1 / Prepare the fluid cell and stretch the nanopore
Wetting the porous lower fluid cell paste with 75 microlitres of 70%
ethanol for 30 seconds helps prevent bubbles from gathering under the
pore.
Remove the fluid in the lower fluid cell before fitting the pore with the serial
number facing upwards. Wind the stretching wheel mechanism clockwise
and use the callipers provided to calibrate the stretch to 47 mm. Enter the
stretch value into the software and click “calibrate stretch”.
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