Izon qNano, User Manual, Page: 37 / 56

BEHAVIOUR OF NANOPORES AT DIFFERENT
STRETCHES
The stretch of the nanopore being used can be altered by winding the
stretching mechanism on the qNano up or down. Rotating clockwise will
increase the applied stretch, while anti-clockwise will decrease it. Choosing a
suitable operating stretch is critical, as this will have an impact on the stability
of the pore.
*Operating at a stretch of 44-45 mm is possible but at low cruciform tension
the pore may exhibit less baseline stability and may be more prone to
blockages. Operating at a stretch of 47-48 mm and greater may work well
but the pore may become problematic for further use at lower stretches. This
is because the cruciform has undergone some structural changes at the high
stretch. On some occasions, excessive stretch can cause a rupture of the pore.
3 /
STRETCH (MM)
40 41 42 43 44 45 46 47 48 49 50 51 52
Winding
below 40 mm
can damage
the winding
mechanism,
and pore will
not be open
Very unstable
recordings,
nanopore
prone to
blocking
*
Optimal operating
stretch *
Very stable
recordings, but
nanopore is at
risk of being
stretched out
of shape
Very stable
recordings,
but nanopore
at risk of
rupturing
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