Invitrogen E-Gel Power Snap Plus, User Manual

Page: 58 / 86

Prepare wells
1.
Open the filter lid of the E
‑
Gel
™
Power Snap Plus Electrophoresis Device and activate the Back
light.
The transilluminator turns off automatically when the filter lid is opened. Press
Back light to
re-activate the blue light transilluminator.
2.
Load 40 µL of deionized water to all recovery wells. Do not allow water to spill over the edge of the
wells.
Collect DNA fragment
1.
Resume the run and carefully observe as the band of interest fully enters the recovery well.
2.
Stop the gel and recover the sample with a pipette. Avoid piercing the agarose.
Some residual DNA will remain visible in the well due to migration into the agarose at the bottom of
the well.
3.
Proceed with downstream cloning workflow. No additional gel-purification is required.
4.
( Optional ) Collect additional DNA bands in the same sample from the recovery well by adding more
water to the recovery well (see “Prepare wells” on page 58 ).
5.
( Optional ) Use the Reverse E
‑
Gel
™
protocol if the band
of interest passes the recovery well (see “Change to
another protocol”
on page 30 ).
Appendix D
E
‑
Gel
™
CloneWell
™
II Agarose Gels
Prepare wells
D
58
E
‑
Gel
™
Power Snap Plus Electrophoresis System User Guide