Prepare the gel
1.
Remove the gel from the package.
2.
Gently remove the combs. Do not allow the combs to bend or create suction in the wells during
removal.
3.
Place the cassette into a E
‑
Gel
™
Adapter, starting from the right edge.
4.
Insert the gel cassette and adapter into the E
‑
Gel
™
Power Snap Plus Electrophoresis Device,
starting from the right edge.
5.
Press down on the left side of the cassette to secure it into the device.
Load samples
1.
Fill all wells of both rows with 50 µL of deionized water.
2.
Load 25 µL of sample with 1X Sample Loading Buffer into wells from the bottom up. Do not
damage the gel or introduce bubbles into the wells.
3.
Load 25 µL of ready-to-use E
‑
Gel
™
1 Kb PLUS
™
Express DNA Ladder into a well.
Appendix D
E
‑
Gel
™
CloneWell
™
II Agarose Gels
Prepare the gel
D
56
E
‑
Gel
™
Power Snap Plus Electrophoresis System User Guide
