Table 5 NGS library size selection reference
Library Size
Target library
peak
Run time to
reference line
Input sample
amount
Stop the run
and collect your
sample when…
Schematic view
Ion PGM
™
System
400-base-read
480 bp
14–20 min
500 ng
500 bp band is
at the
top
of the
exposed
agarose area
50–100 ng
500 bp band
has
just entered
the top edge
of
the
collection
well
300-base-read
390 bp
13–16 min
500 ng
400 bp band is
at the
middle
of
the
exposed
agarose area
50–100 ng
500 bp band is
at the
top
of the
exposed
agarose area
200-base-read
330 bp
12–14 min
500 ng
350 bp band is
at the
top
of the
exposed
agarose area
50–100 ng
350 bp band
has
just
completely
entered the top
edge
of the
collection well
100-base-read
200 bp
11–12.5 min
500 ng
200 bp band is
in the
middle
of
the
collection
well
Appendix E
E
‑
Gel
™
SizeSelect
™
II Agarose Gels
Guidelines for estimating run time
E
66
E
‑
Gel
™
Power Snap Plus Electrophoresis System User Guide
