E
‑
Gel
™
CloneWell
™
II Agarose Gels
E
‑
Gel
™
CloneWell
™
II pre-cast agarose gels can be used with the E
‑
Gel
™
Power Snap Plus
Electrophoresis Device to provide a fast, safe, and effective DNA fragment isolation method for DNA
cloning workflows.
General guidelines
• Load gel within 15 minutes of opening the pouch; run the gel immediately after loading.
• Monitor the band of interest carefully as it migrates near the recovery wells. It may be difficult to
see low amounts of DNA in the well.
•
IMPORTANT!
Always wear Safe Imager
™
Viewing Glasses when viewing the gel with the filter lid
opened.
• For guidance on disposal of used gels, see SYBR
™
Safe DNA Gel Stain (“SYBR
).
Prepare samples
• Prepare up to 25 µL of sample in 1X Sample Loading Buffer (e.g., use 2.5 µL of 10X Sample
Loading Buffer with 22.5 µL total sample).
10X Sample Loading Buffer is provided with E
‑
Gel
™
CloneWell
™
II Agarose Gels.
•
Use the indicated amount of DNA per well
for single or multiple bands.
• Divide samples with higher amounts of DNA across multiple wells.
• Use up to 25 µL total sample volume per well.
• Dilute high salt samples (certain restriction enzyme and PCR buffers) 2- to 5
‑
fold.
Gel type
Amount of DNA per well
Total loading volume
Sample with single band
Sample with multiple
bands
E
‑
Gel
™
CloneWell
™
II
200-800 ng
800 ng
25 µL
D
E
‑
Gel
™
Power Snap Plus Electrophoresis System User Guide
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