16
Bacteria (suspect presence)
When the
Confirm with one of the following: image review, air-dried, stained cytological preparation
(“dry prep”), or urine culture
message appears, bacteria may be present in the sample. The images
should be reviewed to determine if there is sufficient evidence of bacteriuria by identifying uniformly
sized structures or if the small structures are in the form of chains or doublets. If bacteria cannot be
visually confirmed in the images, prepare and review either an air-dried, stained cytological slide
and/or a urine culture due to likelihood of bacteria being found in the sample.
Bacteria possible
Bacteria possible
Bacteria obvious
To perform a dry prep
Performing a dry prep is an extremely cost-effective means of confirming the presence or absence
of bacteria, of differentiating between cocci and short rods, and for characterizing various cellular
elements in the urine sample.
1. Label your slides appropriately.
2. Fill a centrifuge tube with well-mixed, fresh urine taken from the bottom of the sample tube.
3. Centrifuge the sample (and a balance
tube) on the
Urine
setting (or 400 g).
Note:
If your centrifuge does not have
a Urine setting, refer to its operator’s
manual for centrifugation settings and
times.
4. After centrifugation, a concentrated pellet
of formed elements should be visible at
the bottom of the tube. Gently aspirate
the supernatant down to the pellet,
leaving an extremely small amount of
urine in which to resuspend the pellet.
Note:
If the sample is extremely
hypocellular, it may be very difficult to see
the pellet.
5. Lightly flick the bottom of the tube
multiple times with your finger to gently
resuspend the formed elements.
6. Using a new pipette, dispense a drop
of sample on a glass slide, similar to
preparing a blood film.
7. Place a clean glass spreader slide on
your labeled slide, at approximately
30°–40°, in front of the drop of urine.
8. Back the spreader slide into the drop
allowing the material to spread along the
edge of the spreader slide.
6
7
8
Understanding Your Results
Summary of Contents for SediVue Dx
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