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Heraeus CRYOFUGE 6000i, Operating Instructions Manual

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Heraeus CRYOFUGE 6000i Operating Instructions Manual Download Page 45

Maintenance and Service

41

Autoclaving

Observe the permissibility of autoclaving!

CAUTION!
Rotor and carrying buckets must not be autoclaved.  

The Centri-Lab inserts can be autoclaved at 121 °C.

Chemical additions to the steam are not permitted!

Broken glass

Centrifuge glasses have an increasing statistical break quota with increasing ‘g’ size.

CAUTION - WARNING!

Splinters of glass must be immediately and completely removed from the swing bucket / adapter and the
rotor area, as they can scratch the surface area protection or become stuck in the retaining bolts of the
swing bucket rotors and therefore hinder the buckets when swinging out.

Bearing lubrication

CAUTION – LUBRICATE SWINGING BEARING!

The retaining bolts of the swing buckets must always be lightly lubricated, to prevent jerky move-
ments of the swing buckets! They should be lubricated regularly, at least after every 10 runs!

The bolts of the rotor and the retaining grooves of the swing buckets must be thoroughly cleaned with a dry
cloth before lubrication. Under no circumstances are organic solutions, alkaline cleaning agents or cleaning
agents containing scouring powder to be used.

Lubricants are among the accessories (order number 70006692)

Important information!
Molykote and graphite containing lubricants are not permitted!

DO NOT LUBRICATE THE MOTOR BEARINGS !
They are equipped with a lifetime lubricant.

CAUTION!
Ensure that no fluid, and in particular no organic solution enters the gaps of the motor shaft mouth, as this
could wash the lubricant from the bearings.

The felt ring for sealing the bearing gap must be completely soaked with oil, to provide sufficient protection
against any liquid that may leak.

Summary of Contents for CRYOFUGE 6000i

Page 1: ...CRYOFUGE 6000i CRYOFUGE 8500i Operating Instructions ...

Page 2: ... help you to avoid improper operation Therefore always keep the instructions close to the centrifuge Operating instructions which are not accessible cannot prevent improper use and therefore injury or damage to property Please open out On the back of this page you will find an illustration of the operating field with a compilation of the most important func tions ...

Page 3: ...delay Temperature field 25 Temperature display 26 Reference LED Temperature display 27 Selection key for set temperature or excess temperature control 28 Reference LED Excess temperature Adjustment key field 29 Key for set value display and adjustment 30 Direction keys for pre setting the display positi ons 31 and keys for set value adjustment 32 Key for saving the adjusted set values Control key ...

Page 4: ......

Page 5: ...ion 13 Transport and Storage 13 Unpacking the CRYOFUGE 13 Unpacking the Rotor 15 Installation place 17 Connection requirements 17 Start up 17 Opening the lid 17 Installing and Removing the Rotor 19 Handling the wind protection boiler rotor 19 Installing the rotor 19 Lubricating the swing bearing bolts 19 Rotor hood 20 Rotor removal 20 Loading the Rotor 21 General instructions 21 Bucket positions 2...

Page 6: ...peration with saved programs 31 Key switch position 31 Choosing a program 31 Storing programs and starting without pressing the enter key 31 Modifying programs in the RAM memory 31 Additional setting information 32 Input in the curve field 32 Input in the four function field 32 Input in the time field 33 Input in the temperature field 33 Special features 34 Rapid input 34 Acoustic signal transmitt...

Page 7: ...still moving It is prohibited to program any rotor or bucket other than those inserted Exclude any improper operation such as Incorrectly installed centrifuge Operation with casing or parts thereof removed Operating the centrifuge after interference in mechanical or electrical components by non authorised persons Starting the centrifuge with a non permitted rotor Operating the centrifuge with part...

Page 8: ...Safety Measures and Hazards 4 ...

Page 9: ...onmentally friendly refrigerants The central processor monitors the sequencing the speed measurement and control the temperature measurement and control the safety systems and the error messages of the centrifuge An additional microprocessor is responsible for the key and display functions and is also used for saving parameters The practical SEPACONTROL system takes care of the operation and progr...

Page 10: ...locking of the lid does not work To remove the specimen after the rotor has stopped there is a manual opening device In this case proceed as follows Switch off and disconnect the centrifuge from the power supply Ensure that the rotor is at a standstill view window Unlock and open the front door with a square socket spanner Pull down both pull cords on the right the lid catch is mechanically unlock...

Page 11: ...onfirmed in a declaration of conformity The centrifuges bear the CE symbol For operators in Germany the following regulations for the prevention of accidents apply UVV VBG 7z Centrifuges UVV VBG 4 Electrical installations and operating material UVV VBG 20 Refrigerating plants The certification according to DIN ISO 9001 confirms that KENDRO Laboratory Products has an organisa tion which guarantees ...

Page 12: ...Description of Appliance 8 ...

Page 13: ...ays be pro grammed otherwise this could lead to the destruction of the appliance due to incorrectly entered speeds 8 Setting the speed Press the hand symbol key of the 4 function field until the speed LED is alight The speed position last entered flashes and can be changed with the white direction arrows The numerical value rpm can be entered using the arrow symbol keys to enter the RZB value see ...

Page 14: ...Instructions for Rapid Start up 10 ...

Page 15: ...ouble function key start release when the green LED is on stop re lease when the red LED is on Start release Also during the deceleration phase Quick stop At any time using a special key also during the deceleration phase Imbalance stop Automatically when exceeding the permitted imbalance Ambient conditions Indoor use Height up to 2000 m above sea level Max relative humidity 80 up to 31 C linearly...

Page 16: ... delay Temperature or difference between set and actual temperature Acceleration Curves 1 9 can be pre set Deceleration Curves 1 9 and 0 slow down without deceleration cannot be repro duced can be pre set Speed pre setting In the range of 300 rpm up to max bucket speed adjustable in 10 100 or 1000 rpm steps RZB pre setting From the minimum RZB value relating to 300 rpm up to the maximum RZB value ...

Page 17: ...able and even To roll the centrifuge down a free area of at least 2m appliance and rails is required First remove the surrounding carton Then the centrifuge cov ered with corrugated cardboard is visible Illustration 1 View of the CRYOFUGE 6000i 8500i after removing the outer cardboard casing Metal rail Strip to be removed Wooden lath for support After removing the steel straps the 4 securing screw...

Page 18: ...Centrifuge being slowly rolled from the palette by 2 people After installation at the location re attach the stand feet Observe requirements of the place in which the centrifuge is to be installed Screw out the stud bolts put the appliance feet underneath in the centre and align the centrifuge so that all feet are equally burdened carry out the toppling test and the transport rollers are approxi m...

Page 19: ...o unpack the contents proceed as follows 1 Cut and remove the steel straps using snips Illustration 7 2 Cut open the carton along the edges Illustration 8 3 Remove the foam padding and take out the buckets and other accessories Illustration 9 4 Take the lid off the uncovered tubular frame and cut the steel straps using snips and remove them Illustration 10 Illustration 7 Illustration 8 Illustratio...

Page 20: ... The rotor weighs approx 25 kg and is therefore best transported by two people as shown in Illustration 13 WARNING Under no circumstances hold the rotor by the wind boiler and try to transport it in this way The wind protection boiler could bend and then the rotor may no longer be able to rotate perfectly if these instructions are not fol lowed 8 Keep the packaging in case of subsequent transporta...

Page 21: ...ase loading 3 For the connection a CEKON socket 32A 6h 3L N PE and for permanent connection a three pole isolating switch 3 x 63 A is available CAUTION A permanent installation of the electrical wiring must only be carried out by a qualified electrician Start up Connect the plug to the required CEKON socket 32 A or remove the plug and permanently install the cable Switch on the power switch on the...

Page 22: ...Transport and Installation 18 ...

Page 23: ...the primary shaft anticlockwise with the 10 mm socket spanner until it stops 4 Tighten the flanged nut with the 19 mm open jawed spanner in a clockwise direction in the other hand holding the rotor by the rotor cross 5 Position the box level centrally on the flanged nut and accurately level the drive using the feet 6 Securely tighten the counter nuts of the stand feet and screw in the safety hooks...

Page 24: ...ood if not the rotor must not be operated with the hood and only up to 2000 rpm without the hood The hood was newly designed after the introduction of the double blood bags since 1993 and can be ob tained afterwards under order no 75007614 Rotor removal In order to remove the rotor from the primary shaft proceed as follows 1 Remove the hood and put it in a safe place do not place it in the holding...

Page 25: ...ts Only buckets of the same weight class may be placed opposite each other Symmetrical loading The following illustrations show how vessels can be incorrectly or correctly placed in the rotor Illustration 18 not acceptable The retaining bolts are unequally burdened The vessels must be distributed within a bucket so that the load on the retaining bolts is equal For partial loading see illustration ...

Page 26: ...aboratory scales or even better counterbalancing scales are suitable Maximum load At high speeds enormous centrifugal forces occur which stress the arms and other rotor parts Therefore each rotor only has a limited strength and lifecycle HERAEUS rotors are designed so that they have a high reserve of strength even with maximum loads and speeds However the maximum values must not be exceeded as oth...

Page 27: ... of the blood bag is a condition for good separating results It is guaranteed by selecting a suitable blood bag bucket and insert Arrangement of the bags in the centrifuge bucket As in a double blood bag bucket different forces are present in the separating zones in both bags during the deceleration phase at low speed this can lead to deviations in the separating results The larger the difference ...

Page 28: ...hilst the water bag has constantly the same mass distribution during the course of centrifugation the erythrocytes in the blood bag slowly migrate to the periphery and displace the centre of gravity so that the system slowly becomes unbalanced Double buckets must be basically equipped with 2 bags or bag systems in order to avoid hanging crookedly If for example only 2 bags are to be centrifuged th...

Page 29: ...e LED Rotor bucket type order no 20 Reference LED Radius Time field 21 Time display 22 Reference LED Running time 23 Selection key for running time or start delay 24 Reference LED Start delay Temperature field 25 Temperature display 26 Reference LED Temperature display 27 Selection key for set temperature or excess temperature control 28 Reference LED Excess temperature Adjustment key field 29 Key...

Page 30: ...CONTROL Position A Unlimited use The user can use or adjust all the programs 3RVLWLRQ 3URJUDP PHPRU LV VHFXUHG DJDLQVW DGMXVWPHQWV 7KH FXUUHQW FHQWULIXJDWLRQ SURJUDP LQ WKH 5 0 PHPRU FDQ EH DGMXVWHG 3RVLWLRQ 3URJUDP DQG 5 0 PHPRULHV DUH VHFXUHG DJDLQVW DGMXVWPHQWV OO SDUDPHWHUV VHW DUH EORFNHG EXW WKH FXUUHQW FHQWULIXJDWLRQ SURJUDP FDQ VWLOO EH UXQ DV RIWHQ DV QHFHVVDU ZLWK WKH FRQWURO NH V OO DFW...

Page 31: ... centrifugation can be initi ated using the start stop key After the start the lid symbol key is blocked yellow LED is off Stopping After each start the stop release red LED is alight is immediately activated By pressing the start stop key centrifugation is terminated After each stop the green LED is alight again This means that the interrupted cen trifuging process can be continued at any time it...

Page 32: ...LED marks the chosen functional parameter and the set value last entered is shown Calling up the setting position decimal position Move the flashing cursor to the figure to be changed using the arrow keys Positions which cannot be changed are skipped Entering the set value With each touch on an one of the arrow keys the displayed set value is increased or de creased Ending the setting returning to...

Page 33: ...in position 33 under display F Key switch position Changing profile programs is only possible in key switch position A CAUTION The centrifugation process must not be started Pre setting a program number Call up the memory segment F1 using the directional program selection keys Activating programming The memory segment F1 is displayed flashing by pressing the store mode key Entering set values In t...

Page 34: ...As for F1 5 As for F1 F6 F7 F8 F9 F0 results from the course of the curve independently of the pre set time Profile program B 16 min Program level Acceleration curve Deceleration curve Speed rpm Rotor bucket no Time min Temp C F1 5 9 1260 7617 3 20 F2 9 1 860 As for F1 7 As for F1 F3 1 9 1260 As for F1 1 As for F1 F4 9 5 0 As for F1 5 As for F1 F5 F6 F7 F8 F9 F0 results from the course of the curv...

Page 35: ...arting without pressing the enter key By pressing on the start key within 2 minutes the content of the preset program memory position is copied in the RAM memory and immediately started If the start key is not pressed the chosen program is closed without being stored in the RAM memory Modifying programs in the RAM memory Choose the program that is to be changed as described As described in RAM mem...

Page 36: ... the inserted bucket 2 RZB The RZB set value can be entered in steps of 1 10 100 or 1000 from the equivalent of 300 rpm up to the maximum RZB value of the inserted bucket 3 Rotor bucket Pre setting the bucket is only possible before the start of centrifugation The ro tor order numbers are saved in ascending order and can only be entered as integers CAUTION DANGER The order number last 4 digits of ...

Page 37: ...he start delay set the timer is activated only after pressing the start key In the four function field delay is also shown for the duration of the start delay If no start delay is necessary the setting is displayed Input in the temperature field 1 Sample temperature The set value of the sample temperature can be set in a range of 20 C to 40 C in 10K or 1K steps 2 Excess temperature control The DT ...

Page 38: ... centrifugation a steady tone is produced which can be switched off by quickly pressing any key This acoustic signal can be deactivated for all further centrifugations by pressing the enter key in the actual value mode By pressing the key Alert off is briefly dis played This switching off can be undone by pressing enter again display now Alert on or by turning the appliance off and then on again D...

Page 39: ...ce of curves Curve No 9 has the shortest acceleration or deceleration time curve no 1 has the longest acceleration or deceleration time Deceleration curve 0 means non decelerated slow down and can therefore not be re produced The quickest deceleration can be carried out using the quick stop key in which the rotor is brought to a standstill with the maximum brake force possible Whirl production on ...

Page 40: ...Acceleration and Deceleration Curves 36 PLQ P L Q Acceleration curves Cryofuge PLQ P L Q Deceleration curves Cryofuge ...

Page 41: ...pty buckets should be pre heated before centrifuging begins For this purpose the following procedure is recommended 1 Enter in the order number of the inserted bucket 2 Enter the required sample temperature 3 Set DT to 4 Set speed to 2000 rpm WARNING For bucket no 7719 nmax 1500 rpmis the maximum speed 5 Set running time to 30 min 6 Remove rotor hood and then press the start key The speed set may ...

Page 42: ...Instructions for Temperature Control 38 ...

Page 43: ...entrifuge and its accessories For cleaning use only the cleaning and disinfection procedures stated in these operating instruc tions Cleaning Disconnect from the power supply before cleaning Maintaining the centrifuge generally means keeping the housing the rotor chamber the rotor and the ac cessories clean This should be done at regular intervals or whenever necessary This is for hygienic rea son...

Page 44: ...power supply pull the mains plug 2 Remove the centrifuge vessels and adapters and dispose of or disinfect them 3 Treat the rotor and the rotor lid according to the instructions for the disinfectant either submerse in solu tion or spray The given time to take effect must be observed 4 Afterwards rinse the rotor and rotor lid well with water 5 Dispose of the disinfectant solution according to curren...

Page 45: ...t always be lightly lubricated to prevent jerky move ments of the swing buckets They should be lubricated regularly at least after every 10 runs The bolts of the rotor and the retaining grooves of the swing buckets must be thoroughly cleaned with a dry cloth before lubrication Under no circumstances are organic solutions alkaline cleaning agents or cleaning agents containing scouring powder to be ...

Page 46: ...Maintenance and Service 42 ...

Page 47: ...ious causes and if possible eliminate In other cases request service Lid does not open Pressing on lid open key to no effect Lid is not engaged properly or lid is distorted 1 Check that the appliance is connected to the power supply and that it is switched on display s lights are on 2 If this has no effect you can open the lid using the mechanical emergency unlocking see page 6 Loud running noises...

Page 48: ... tion until it comes to a standstill Appliance is not oper able Defective speed detection Turn the appliance off and on again If the fault continues request customer service system check LED is alight E 04 intermittent tone The rotor is slowing down without decelera tion until it comes to a standstill Appliance is not oper able Temperature measuring malfunction sensor broken Turn the appliance off...

Page 49: ...stem check LED is alight E 17 intermittent tone Lid does not open Lid is blocked or jammed Press the lid down once at the front in the centre and then press the lid open key again Otherwise see Mechanical emergency lid opening page 6 system check LED is alight E 19 intermittent tone During the self test after switching on the centri fuge Incorrect NV RAM or keypad system check LED is alight E 22 i...

Page 50: ...iagnosis messages Non intermittent or intermittent alarm tone Error messages in the display note down E_xx error no Unusual parameter displays speed RZB time rotor temperature and the malfunction behaviour Unusual noises in when starting up accelerating controlling or decelerating Slowing down with or without deceleration etc before you inform the service Furthermore details of the software versio...

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Page 52: ...N 50 60 Hz 21 A 4400 W 176 kNm 20 A 6 3 A CRYOFUGE 6000i 7500 7526 230 V 1N 50 Hz 19 5 A 3600 W 1 176 kNm 20 A 6 3 A CRYOFUGE 8500i 7500 7550 400 V 3N 50 Hz 24 A 5400 W 291 kNm 20 A 6 3 A CRYOFUGE 8500i 7500 7551 230 V 3N 50 60 Hz 26 A 6000 W 291 kNm 20 A 6 3 A 87 21 Q FDVHV RI IDXOWV WKH DSSOLDQFH IXVHV PXVW RQO EH UHSODFHG E DQ DXWKRULVHG VHUYLFH WHFKQLFLDQ 6LQJOH SKDVH DSSOLDQFH ZLWK UHGXFHG UH...

Page 53: ... 300 Telefax 49 61 81 3 57 39 Domestic sales 49 61 81 3 57 49 Export sales 49 55 22 31 62 76 Service Telephone 31 62 22 Service In the interest of continuous product development we reserve the right to make changes without express notice 20056907 Cryofuge_UK 03 00 Printed in Germany ...

Page 54: ...e 26 Profile program A 24min Program level Acceleration curve Deceleration curve Speed rpm Rotor bucket no Time min Temp C As for F1 results from the course of the curve independently of the pre set time Profile program B 16 min ...

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