Heraeus CRYOFUGE 6000i, Operating Instructions Manual

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23
Special Features for Working with Blood Bags
Inserting blood bags
Correct position of the blood bag
The flexibility of a blood bag can lead to difficulties during inserting in and removing from the centrifuge
bucket/insert.
If the bucket is too narrow, the bags are difficult to insert. If the bucket is too wide, then the material can
form creases and folds, can overstretch or tear.
Therefore the bag must sit firmly in the insert in order to avoid folding. "Nests" can form in the folds, i.e. cells
can accumulate and evade the sedimentation process. This leads to poor results.
Furthermore, it must be ensured that the tube connections in which cells are also found, are standing up-
right. Then the cells can be separated and do not contaminate the plasma during the subsequent squeezing
process.
The correct position of the blood bag is a condition for good separating results. It is guaranteed by selecting
a suitable blood bag bucket and insert.
Arrangement of the bags in the centrifuge bucket
As in a double blood bag bucket different forces are present in the separating zones in both bags during the
deceleration phase at low speed, this can lead to deviations in the separating results.
The larger the difference, the larger the distance between the bags in the bucket. Therefore it is recom-
mended that the bags filled with blood be arranged in the double buckets in the centre opposite one an-
other, and the satellite bags in the outer area.
Illustration 21
Use of plastic inserts
In order to facilitate the transportation of blood bags before and after centrifuging, plastic inserts can be
used. The bags are placed inside these inserts and placed directly in the blood bag buckets of the centri-
fuge.
After centrifuging, the inserts with the bags are removed. The bags can easily be removed from the inserts
and inserted in the squeezing device.