Heraeus CRYOFUGE 6000i, Operating Instructions Manual

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24
Centrifuging blood bags
When centrifuging blood bags, the following must be ensured:
The balance of the centrifuge operation depends to a great extent on the
distribution of weight in the opposing buckets.
The larger the difference in weight of the load, the stronger the oscilla-
tions of the appliance and as a result of this, the stronger the whirling up
of already separated areas.
œ
Balance opposing buckets well
(maximum tolerance 5 grams)
œ
Lay the tubes hanging from the bags downwards, so that they cannot
be thrown around and torn during operation.
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Avoid an uneven number of blood bags, because then the balance will
need to be made up e.g. with water bags, which has the following dis-
advantage:
Whilst the water bag has constantly the same mass distribution during
the course of centrifugation, the erythrocytes in the blood bag slowly
migrate to the periphery and displace the centre of gravity so that the
system slowly becomes unbalanced.
Double buckets must be basically equipped with 2 bags or bag systems,
in order to avoid hanging crookedly. If, for example, only 2 bags are to be
centrifuged, these should be inserted opposite one another in the inside
of the buckets, and water bags should be placed in the outer positions
(Illustration 24).
Blood bag
Water bag
Three water bags are required per blood bag (Illustration 25).
With multiple bags (double, triple or quadruple bags), the blood removing
bag should be placed in the central area, next to the dividing wall.
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