HEKA Elektronik EPC 10 USB, Hardware Manual, Page: 35 / 81

5.1 EPC 10 USB Window 29
current potential has been set correctly). The ”V-membrane” label is converted to ”I-membrane” in current clamp
mode.
I-mon: Displays the actual measured pipette current.
Figure 5.4: Input monitor
V-mon: Displays the actual measured pipette voltage after correcting for liquid junction potentials and offsets
(provided the zero-current potential has been set correctly). This may differ (temporarily) from the holding voltage
(e.g., during long stimulation pulses) as it indicates the average sum of
V-membrane and the scaled stimulus voltage.
R-memb: The seal resistance ( R-membrane ) is determined from the current sampled during the baseline and the
second half of the Test Pulse . R-membrane can be encoded into a tone using the Sound feature (see below).
Input ADC: The Oscilloscope can display the following signals (
EPC 10 USB
Single):
Figure 5.5: Input ADC
ˆ
Imon1: Current monitor 1 (after Filter 1 )
ˆ
Imon2: Current monitor 2 (after Filter 2 )
ˆ
Vmon: Voltage monitor output
ˆ
AD 0. . . 4: Any of the other AD channels.
Recording Mode: Sets the Recording Mode. For details on the various recording modes see chapter 6 on page 39.
Figure 5.6: Recording Mode
ˆ
In Out: Sets the Inside Out mode.
ˆ
On Cell: Sets the On Cell mode.
ˆ
Out Out: Sets the Outside Out mode.
ˆ
Whole Cell: Sets the Whole Cell mode.
ˆ
C-Clamp: Sets the Current Clamp mode.
Test Pulse: Test Pulses are added to the holding potential and applied to the pipette; the current responses
are sampled and displayed. Two built-in Test Pulse types are available: single or double pulse. Additionally any
user-defined pulse pattern can be used as a Test Pulse . Test Pulses are applied at maximal rates depending on the
duration specified.
Amplitude / Length: Duration and amplitude of built-in Test Pulses can be specified in the dialog. The
minimum pulse duration is 0.1 ms with 1000 points sampled per pulse.
Figure 5.7: Test Pulse parameters
http://www.heka.com