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Hach DR 5000, User Manual

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Hach DR 5000 User Manual Download Page 134

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Limited Warranty

Summary of Contents for DR 5000

Page 1: ...DOC022 53 00654 DR 5000 USER MANUAL January 2008 Edition 2 HACH Company 2007 2008 All rights reserved Printed in Germany ...

Page 2: ...2 ...

Page 3: ...21 5 1 3 Main Menu 22 5 2 Instrument Setup mode 23 5 2 1 Operator ID 23 5 2 2 Sample ID 24 5 2 3 Date and time 25 5 2 4 Display and sound preferences 25 5 2 5 Lamp Control 26 5 2 5 1 VIS Lamp and UV Lamp 26 5 2 5 2 Effect caused by the lamp settings on the Measuring Mode 27 5 2 6 PC and printer 29 5 2 6 1 Printer setup 29 5 2 6 2 Print data 31 5 2 6 3 HACH Data Trans 31 5 2 7 Password 32 5 2 7 1 P...

Page 4: ...date edit barcode tests 54 5 5 6 1 Manual update of a barcode test 54 5 5 6 2 Update an existing barcode test 57 5 5 6 3 Program a new test 58 5 5 7 Upgrade of the instrument software 58 Section 6 Advanced Operations 61 6 1 User Programs 61 6 1 1 Program a user method 61 6 1 1 1 Single wavelength settings 63 6 1 1 2 Multi wavelength settings 64 6 1 1 3 Calibration settings for single and multi wav...

Page 5: ...2 Time course scan reading 102 6 7 3 Analysis of time course data 103 6 7 3 1 Navigation of a time scan or a time scan analysis 104 6 8 System checks 105 6 8 1 Instrument information 105 6 8 2 Upgrade of the instrument software 106 6 8 3 Optical checks 106 6 8 3 1 Wavelength check 107 6 8 3 2 Noise check 108 6 8 3 3 Stray light check 110 6 8 3 4 Absorbance check 111 6 8 3 5 Drift check 112 6 8 3 6...

Page 6: ...6 Table of Contents ...

Page 7: ...ometric accuracy 5 mAbs at 0 0 0 5 Abs 1 at 0 50 2 0 Abs Photometric linearity 0 5 2 Abs 1 at 2 Abs with neutral glass at 546 nm Stray light KI solution at 220 nm 3 3 Abs 0 05 Data storage 1000 measured values result date time sample ID user ID User programs 50 Physical and environmental specifications Width 450 mm 17 7 in Height 200 mm 7 9 in Depth 500 mm 19 7 in Weight 15 5 kg 34 2 Ibs Operating...

Page 8: ...8 Specifications ...

Page 9: ...hasis Note Information that supplements points in the main text 2 1 2 Precautionary labels Read all labels and tags attached to the instrument Personal injury or damage to the instrument could occur if not observed A symbol if noted on the instrument will be included with a danger or caution statement in the manual This symbol if noted on the instrument references the instruction manual for operat...

Page 10: ...ts can be dangerous Users of this product are advised to familiarize themselves with safety procedures and the correct use of chemicals and to carefully read all relevant Material Safety Data Sheets Normal operation of this instrument may involve the use of hazardous chemicals or biologically harmful samples The user must observe all cautionary information printed on the original solution containe...

Page 11: ... Time Course Mode The DR 5000 is used for testing in visible and ultraviolet wavelengths A gas filled tungsten lamp produces light in the visible spectrum 320 to 1100 nm and a deuterium lamp available produces light in the ultraviolet spectrum 190 to 360 nm The DR 5000 Spectrophotometer provides digital readouts in direct concentration units absorbance or percent transmittance When a user generate...

Page 12: ...12 General Information ...

Page 13: ...n and accurate results Place the instrument firmly on an even surface Do not push any objects under the instrument Maintain an ambient temperature of 10 to 40 ºC 50 to 104 ºF for proper instrument operation The relative humidity should be less than 80 moisture should not condense on the instrument Leave at least a 15 cm 6 in clearance at the top and on all sides for air circulation to avoid overhe...

Page 14: ...erface is used for communications with a PC The optional Hach Data Trans software see section 5 2 6 3 on page 31 must be installed on the PC for this use A USB hub may be used to connect several accessories at a time Note USB cables must not be longer than 3 meters 10 feet These USB interfaces enable data and graphics to be output to a Printer and a PC and upgrade of instrument software see sectio...

Page 15: ...wing cell types 10 mm square cells 20 50 mm rectangular cells 1 round cells 1 square cells Only one cell type can be used for a measurement On the top and bottom of the Multi Cell Holder are a variety of openings to accommodate different types of cells Beside each opening is printed the type of cell for which it is intended Optionally there is a 100 mm adapter available Figure 2 Cell compartment 1...

Page 16: ... 100 mm adapter optional Figure 3 Multi Cell Holder Top and Bottom 1 10 mm square cell 4 1 round cell 2 20 mm rectangular cell 5 1 square cell 3 50 mm rectangular cell Figure 4 100 mm Cell Adapter 1 100 mm rectangular cell ...

Page 17: ...he correct opening for the selected cell type in the Multi Cell Holder 3 Insert the Multi Cell Holder in the cell compartment in such a way that the name of the selected cell type can be read directly and the cell opening is at the front Secure the holder with two locking screws Figure 5 Figure 5 Installation Multi Cell Holder ...

Page 18: ... path 1 Deuterium lamp 10 Exit slit 2 Lamp selection mirror 11 Cell compartment 3 Tungsten lamp 12 Lens 4 Mirror 13 Round cuvette vial compartment 5 Grating 14 Measurement element 6 Reference element 15 Monochromator 7 Mirror 16 Mirror 8 Splitter mirror 17 Mirror 9 Filter 18 Entrance slit ...

Page 19: ...rt automatically Changing the language setting The instrument functions in the selected language until the option is changed 1 While turning the instrument on touch the screen at any point until the list for selecting a language appears about 30 seconds 2 Select the required language 3 Press OK to confirm The test program subsequently starts automatically 4 3 Self Check Each time the instrument is...

Page 20: ...20 Start Up ...

Page 21: ... when programming the instrument Unavailable options are disabled grayed out The icons on the right and left of the screen are described in Table 1 The central keypad changes to reflect the chosen entry mode Press a key repeatedly until the desired character appears on the screen A space can be entered by using the underscore on the YZ_ key Note A USB keyboard with US keyboard layout or a USB Barc...

Page 22: ...through the sample and reaches the detector is measured Concentration measurements A concentration factor can be entered to enable the measured absorbance values to be converted into concentration values Multi Wavelength In the multi wavelength mode absorbance Abs or percentage transmittance T is measured at up to four wavelengths and absorbance differences and absorbance relationships are calcula...

Page 23: ...nformation 5 2 1 Operator ID Use this option to enter up to 30 sets of operator initials up to 5 characters each into the instrument This feature helps record which operator measured each sample 1 Press Operator ID in the Instrument Setup 2 Press New to enter a new Operator ID 3 Use the alphanumeric keypad to enter a new Operator ID 4 Press OK to confirm 5 The display shows the chosen Operator ID ...

Page 24: ...nner see Section 9 on page 129 is connected Sample IDs can also be scanned 4 Press OK to confirm 5 To number the Sample IDs sequentially e g Inflow 01 etc select Add Number Use the arrow keys to specify the first number of the sequence Use the key between the arrow keys to enter the first number of the sequence using the alphanumeric keypad 6 Press OK to return to Instrument Setup 7 The Sample ID ...

Page 25: ...mber of fields Press the appropriate field and use the arrow keys to change the value 3 Press OK to confirm The instrument will return to Instrument Setup 5 2 4 Display and sound preferences 1 Press Display Sound in the Instrument Setup The following options will be displayed Display Contrast Adjusts the display brightness to suit lighting conditions Screen touch Activates Deactivates a short beep...

Page 26: ... to 360 nm either the deuterium lamp UV lamp or the tungsten lamp can be used for measurements The lifetime of the lamps is influenced by on off operation and the length of time in use Typical use is to turn the instrument on for the entire 8 10 hour shift and then off until the next day Note In general avoid on off cycles of the lamp this shortens the lamp s life span For maximum life turn the la...

Page 27: ...se the alphanumeric keypad to enter the maximum wavelength for UV operation 9 Touch OK to confirm and return to Lamp Control 10 Touch OK to confirm and return to Instrument Setup Note The selected program that requires a lamp has highest priority If the lamp in the Lamp Control was turned off the lamp will be turned on automatically if it is needed for the stored program 5 2 5 2 Effect caused by t...

Page 28: ...rmed up and ready the UV icon stops flashing Note If both lamps are On the icon UV VIS is displayed in the selected Measurement Mode Note Alternatively you can touch the UV or VIS Lamp icon on the measurement screen to change the actual setting ...

Page 29: ...e A USB memory stick is used to upgrade data and software see section 6 8 2 on page 106 Important Note A screened USB cable must not be longer than 3 m 1 Press PC Printer in the Instrument Setup A list with information about the connections opens 5 2 6 1 Printer setup For reasons of compatibility the printer language must be HP PCL 3 2 Press Printer 3 Press Setup to display the Printer Setup scree...

Page 30: ...n off is available 4 Select Auto Send On to send all measured data automatically to the Thermal printer Note The option Auto Send is not available for any other printer e g ink jet printer 5 Press Resolution to select the print quality Select between 100 dpi 150 dpi and 300 dpi 6 Press OK to confirm Note Press OK again to return to the PC Printer menu ...

Page 31: ... section 5 3 1 2 on page 35 4 Press the PC Printer icon to send the data table curve immediately to the printer 5 Highlight Single point or Filtered data or All data and press OK to confirm Sending Data is displayed until the data have been printed 5 2 6 3 HACH Data Trans The optional HACH Data Trans software must be installed on the PC for the subsequent to process for measurement data 1 Press PC...

Page 32: ...7 Password The Password menu contains a variety of security settings to control access to various functions For example prevent unauthorized changes to stored programs or instrument configurations 1 Press Password in the Instrument Setup menu 2 In order to highlight the Security List assign a password Press Set Password 3 Use the alphanumeric keypad to enter a new Password up to 10 characters each...

Page 33: ...u 7 Press On to highlight the new settings of the Security List 8 Enter the new Password again to confirm 9 Press OK to return to Instrument Setup Note The alphanumeric keypad to the Password inquiry appears when a user tries to reach a locked setting 5 2 7 1 Password deactivation 1 Press Password in the Instrument Setup 2 Use the alphanumeric keypad to enter the former Password and press OK to co...

Page 34: ...34 Standard Operations 3 Press Off to deactivate the settings of the Security List 4 Press OK to return to Instrument Setup Note Use this function to delete the former Password or to enter a new one ...

Page 35: ...hich case the user has to decide which data to store 1 Press Store On Off in the Options menu With the Store On setting all measurement data are stored automatically With the Store Off setting no measurement data are stored However this setting can be changed to Store On in the result display through configuration The reading currently shown in the display is then stored Note When the instrument s...

Page 36: ... a USB memory stick to a file named DATALOG The file can then be processed using a spreadsheet program The file name will be formatted as DLYear_Month_Day_Hour_Minute_Second CSV To send data to a Printer see section 5 2 6 2 on page 31 1 Plug in the USB device Figure 1 on page 14 2 Press Recall Data from the Main Menu Press Options and then the PC Printer icon 3 Select the data to send to the memor...

Page 37: ...om the data log 1 Press Recall Data in the Main Menu 2 Press Data Log Options Delete 3 Highlight Single Point or Filtered data or All data and press OK to confirm Note The number in parentheses is the total number of data sets assigned to this selection ...

Page 38: ...age from wavelength scan or time course 1 Press the Store icon in the Options menu after a reading is taken The Store Data list will be displayed 2 Press Store to save the current scan to the highlighted numbered line A scan can also be overwritten 5 3 2 2 Recall stored data from wavelength scan or time course 1 Press Recall Data in the Main Menu a Select Wavelength Scan or Time Course to recall d...

Page 39: ...the Recall Data list 5 3 2 3 Send data from wavelength scan or time course There are two ways to recall sent data to a USB memory stick printer or PC with Hach Data Trans Option 1 1 Press Recall Data in the Main Menu and then Wavelength Scan or Time Course 2 Press Options and then the PC Printer icon to send the data to a USB memory stick to a printer or to a PC with Hach Data Trans ...

Page 40: ...ite appears when the files were already stored Press OK to overwrite the stored data Option 2 1 Press Wavelength Scan or Time Course and then Options More Send Data to send the data to a USB memory stick or to a printer When a printer is connected select how to send the data to the printer graph table or both graph and table When a USB memory stick is connected the files will be automatically sent...

Page 41: ...ngth scan or time course 1 Press Recall Data from the Main Menu and then Wavelength Scan or Time Course or Options More Recall Data A listing of the stored data is displayed 2 Highlight any data to delete 3 Press Delete in the Options menu and press OK to confirm ...

Page 42: ... highlight the program or press Select by number to search for a specific program number Use the alphanumeric keypad to enter the test number and press OK 6 Press Start to run the program After a program is selected the screen for that parameter will appear Note All corresponding data wavelength factors and constants are already preset 7 Follow the chemical procedures described in the correspondin...

Page 43: ...iluted by a factor of 2 enter 2 The default setting of the dilution factor is turned off Note When a dilution is in effect the dilution icon will appear on the display Standard Addition This enables the accuracy of the measurements to be checked The working procedure for a test parameter contains a detailed explanation of how to use this function Standard Adjust The working procedure for a test pa...

Page 44: ...se to view the measurement screen while the timer is running The time will be shown left side bottom instead of the date Note Press Cancel to stop the timer midway through the countdown Note The Timer will beep when the end of the running time is reached A general purpose timer is also available in many programs When the timer icon is visible press the icon and select General Timer A new screen wi...

Page 45: ...he reading sequence is started automatically 2 Insert the sample cuvette into the cuvette sample cell holder 3 Press Options and then the Store icon to store the displayed data in the Data Log Note For data storage see section 5 3 2 on page 38 5 4 5 Set the dilution factor The Dilution Factor function is used to calculate the original concentration of a sample that has been diluted by a known rati...

Page 46: ...Check section of written procedures often suggests a standard solution concentration for this purpose Read a standard before setting Standard Adjust to On 1 Follow the entire procedure using a known standard for the sample 2 After reading the concentration press Options More Standard Adjust 3 If Standard Adjust is set to Off turn it On The Current Reading will show the concentration The box on the...

Page 47: ...he list Note When the program is exited the evaluation form reverts to the standard setting An alternative way of changing the standard setting 1 Press Options More Chemical Form 2 Select the Chemical Form Note The stoichiometric conversion of the measurement result is carried out automatically Note The selected Chemical Form will appear on the display Test results will be calculated and displayed...

Page 48: ...ell compartment Press Read The result is displayed 4 Press Options More Reagent Blank 5 Press On to highlight the Reagent Blank function 6 The concentration shown on the key is the measured value of the reagent blank To use this value for more analyses of this parameter press OK To enter a previously recorded reagent blank value press the key and use the alphanumeric keypad 7 Press OK Note The Rea...

Page 49: ...e section 5 3 2 on page 38 5 4 10 Add stored programs to the favorite programs list The Favorites menu simplifies test selection by creating a list of the most frequently used tests from the Stored Programs and User Programs 1 Press Stored Programs in the Main Menu The Stored Programs list will appear 2 Highlight the selection by pressing it or Select by Number to search for the program by number ...

Page 50: ...rrect wavelength for the analysis and calculates the result immediately with the help of the stored factors In addition measured values are recorded at 10 different positions during a rotation A special outlier elimination program is run and then the average of the measured values is calculated Cuvette vial errors and soiling are recognized and highly precise results are obtained Figure 7 Cell com...

Page 51: ...the result 5 5 2 Select the measuring range Some tests can be used for different measuring ranges After the sample cuvette vial has been inserted a list of the different measuring ranges is displayed Select the required measuring range by highlighting the appropriate line Press Start Permanent if this measuring range is to apply to all subsequent measurements Changing the standard setting In the r...

Page 52: ...close the cell compartment 2 In the result display press Options More Chemical Form 3 A list of available evaluation forms appear Select the new default setting 4 Press Save as Default The current result and all further measurements will be displayed in the new chemical form 5 5 4 Basic test specific and sample specific data settings Press Options to change test or sample specific settings Table 5...

Page 53: ...ly timed e g reaction times wait times etc can be exactly specified When the specified time has elapsed an acoustic signal is emitted The use of the timer has no influence on the measurement program Dilution Factor Off On A corrective dilution factor can be entered in order to take account of certain properties The number entered at the dilution factor prompt will be multiplied by the result to co...

Page 54: ...ame Name of the analysis parameter Version An abbreviation assigned by the user or the version number etc is entered here Measurement process Exact definition of the test the number of wavelengths at which measurements are carried out the number of absorbance measurements needed the keys to be used any waiting periods between measurements etc Formula Definition of the evaluation formulas with whic...

Page 55: ...ta overview and press Edit 2 Press Edit select the sequence to be edited and press Delete 3 Press New and use the alphanumeric keypad to enter the process indicated in the working procedure Enter the Formula Concentration Units Designation Measuring Ranges For detailed information on entering the Formula see section 6 1 2 on page 72 Check the working procedure in advance to determine whether this ...

Page 56: ...ic keypad to enter the data specified in the working procedure for F1 F2 λ1 U1 etc Confirm each entry with OK Abbreviation of Variables F1 Factor 1 F2 Factor 2 λ1 Wavelength 1 U1 Conversion Factor 1 for the first chemical form U2 Conversion Factor 2 for additional chemical forms etc Timer 1 Timer 2 Timer 3 and Timer 4 The timers are activated or deactivated with the control boxes on the left of th...

Page 57: ...ding line and press Edit 5 When finished programming press Store and then Cancel to return to the User Programs menu 6 Press Start to complete the test with the new data Option 2 Manual check revision of test data If an incorrect entry was made during the data update and is not recognized via the barcode of the cuvette sample vial e g an incorrect measuring range another option is available to che...

Page 58: ...ations of the test that is to be programmed and press Edit 5 To finish programming press Store and then Cancel to return to the Main Menu 6 Press Start to run the test with the new data 5 5 7 Upgrade of the instrument software To obtain the software for the update from the Internet at www hach com 1 Go to http www hach com 2 On the DR 5000 product page click Lab System Software Software Update Dow...

Page 59: ...59 Standard Operations 6 Press OK to return to the System Checks menu Note When the instrument software has been updated a prompt to restart the instrument is displayed ...

Page 60: ...60 Standard Operations ...

Page 61: ...thods and tests Press User Programs in the Main Menu and then Program Options The Program Options menu contains several input and editing options Table 7 6 1 1 Program a user method All input steps and their significance and options are explained in the following sections 1 Select New in the Program Options menu Table 7 Program options user programs Options Description 1 New Select New to program ...

Page 62: ...am data Program Type 6 Select the required option Table 8 and press Next 7 If the Single Wavelength section 6 1 1 1 on page 63 or Multi Wavelength section 6 1 1 2 on page 64 is selected define the unit wavelength absorbance formula wavelength λx concentration factor Kx concentration resolution chemical form and calibration equation More information on Free Programming parameters see section 6 1 2 ...

Page 63: ...uded in this list can be added in the edit program under Program Options Edit Select Units Edit and then New Wavelength single wavelength program type Use the alphanumeric keypad to enter the measurement wavelength The entered wavelength must be in the range from 190 1100 nm Press Next to proceed Concentration resolution number of decimal places Select the required number of decimal places from th...

Page 64: ...red unit from the list and press Next Note Units of measure not included in this list can be added in the edit program under Program Options Edit Select Units Edit and then New Absorbance formula multi wavelength program type The Absorbance Formula menu is used to define the wavelengths and the coefficients used in the formula The absorbance formula defines the calculation for the multi wavelength...

Page 65: ...ve been entered The wavelengths must be in the range from 190 1100 nm Press OK Concentration Factor Kx Multiplication factor for converting absorbance values into concentration values Press a factor key and use the alphanumeric keypad to enter a factor If the formula includes more than one factor press another factor key and enter another factor and repeat until all the factors have been entered P...

Page 66: ...e Descriptions Enter values A calibration table is created by entering the concentration values and the absorbance values of the analyte solution The absorbance values are plotted versus standard concentrations and the calibration curve is displayed as a graph page 66 Read Standards A calibration table is created by entering the concentration values of the standard solutions and then measuring the...

Page 67: ...n the data have all been entered press Graph Note The correlation coefficient r2 is shown on the left below the axes 5 The linear equation corresponds to the standard setting Press Next Curve to display the polynomial 2nd order curve Press Next Curve again to display the polynomial 3rd order curve 6 Press Force 0 to change the setting from Off to On The curve now passes through the origin of the c...

Page 68: ... second standard solution into the cell compartment Press Read Repeat this sequence until all the standard solutions have been measured maximum of 24 solutions The entered and measured data are displayed in the table Note To delete a standard concentration highlight the appropriate line and press the Delete icon The timer icon shown in the display helps to ensure when necessary that the steps of a...

Page 69: ...d Go to section 6 1 1 4 on page 70 Calibration by entering the formula 1 Press Enter Formula and press Next 2 Press the formula key A list of available formulas linear and 2nd and 3rd order polynomial is displayed Up to 4 coefficients can be entered depending on the selected formula Press the required formula 3 Depending on the selected formula the required coefficients a b c are displayed Press t...

Page 70: ...nt to another create a new folder on the USB memory stick named dbhl and copy the lst files into this folder see section 6 8 2 on page 106 6 1 1 5 Additional user defined parameters and functions In addition to the previously defined basic data additional parameters and functions can be defined for user programs measuring range upper and lower limits timer functions chemical forms Upper and lower ...

Page 71: ...e entered in mm ss Chemical form 2 chemical form 3 chemical form 4 If a Chemical Form 1 has been defined up to three additional alternative forms can be entered here 1 Highlight the appropriate line in the overview of the program data and press Edit 2 The chemical forms are activated or deactivated with the check boxes in the left part of the display 3 Press the left key to enter another chemical ...

Page 72: ...be completed Are waiting times necessary between measurements Table 10 Definitions of the program points Program point Description Name Name of the analysis parameter Version An abbreviation or version number assigned by the user is entered here Measurement Process Exact definition of the test the number of wavelengths at which measurements are made the number of absorbance measurements needed the...

Page 73: ...leted 1 Highlight the Measurement Process line in the data overview and press Edit 2 Press Edit again and then New Content and definition of the keys Z key Zeroing 1 Press the Z key to program a zero measurement Confirm with OK 2 Press New and then Zeroing and use the alphanumeric keypad to enter the wavelength at which the zero measurement is to be completed Press OK and confirm the input by pres...

Page 74: ...d at a number of wavelengths repeat the above two steps for each wavelength Note The entered measurement sequence is displayed key Elements of the measurement sequence that are to be repeated are placed in brackets The left bracket marks the start of the sequence that is to be repeated and the right bracket marks the end Note The key showing the right bracket remains inactive until a left bracket ...

Page 75: ...lay The data overview is then displayed 6 1 2 3 Enter the calibration formula evaluation formula The calibration formula evaluation formula defines the calculation and display of intermediate and final results The previously defined elements of the measuring sequence are the basis for calculating the concentrations Enter calibration formula C1 1 Highlight the Formula line in the data overview and ...

Page 76: ...s the most recently entered element of the formula Table 11 Edit formula key descriptions Screen Key Description Abs Variables Press the Abs Variables key to select from the displayed list the required element of the defined measuring sequence and therefore the corresponding measurement wavelength so that this can be taken into account in the formula New Number Press New Number to enter a new fact...

Page 77: ...is mathematically permissible in the defined formula this also applies to the basic mathematical operations The following mathematical operations are available Addition Subtraction Division x Multiplication Exponent Ln Natural logarithm Log Common logarithm Press to include logic statements links conditions in the formula The following functions are available Equal to Less than Greater than Less t...

Page 78: ...ified in the working procedure for F1 F2 l1 U1 etc Press OK to confirm each entry Abbreviation of Variables F1 Factor 1 F2 Factor 2 λ1 Wavelength 1 U1 Conversion Factor 1 for the first chemical form U2 Conversion Factor 2 for the further chemical form etc 6 1 2 5 Save a free programming user program 1 Press Store to save the entered data The data can be stored under any data point Measurement sequ...

Page 79: ...t The most frequently used tests methods in the User Program menu can also be added to the list of favorites to simplify their selection 1 Press User Programs in the Main Menu The User Programs list will appear 2 Highlight the selection by pressing it or press Select by Number to search for the program by number Note Use the scroll bar to scroll through the list quickly Use the alphanumeric keypad...

Page 80: ... to confirm An overview of the specifications of the programmed test is displayed More information about the input options is provided in the section 6 1 2 on page 72 6 1 4 3 Delete 1 Press Delete and press OK to confirm The program is deleted from the list of User Programs Note If the stored program is deleted in User Programs it will also be deleted in Favorites Programs ...

Page 81: ... search for the program by number Use the alphanumeric keypad to enter the test number program number and confirm the input by pressing OK 3 Press Start 6 2 2 Delete a favorite program 1 Press Favorite Programs in the Main Menu The Favorite Programs list will appear 2 Highlight the selection by pressing it Note Use the scroll bar to scroll through the list quickly Note Press Select by Number to se...

Page 82: ...the sample The detection rate should be close to 100 Measures to identify other interference factors Checklist 1 Check if the procedure is completed correctly a Are the reagents added in the correct order b Is enough time allowed for color development c Is the correct glassware in use d Is the glassware clean Detection rate Conclusion 100 Probability that the measurement results are correct is hig...

Page 83: ...by step to an already analyzed sample The sample is measured again after each addition Sample volume A defined volume of a standard solution of known concentration is added to the volume of sample specified in the procedure and the sample is measured after each addition In most cases three different standard solutions are prepared and the procedure is repeated for each of them Note The units and c...

Page 84: ...th the instructions in the Procedures Manual When the measurement is complete leave the sample cuvette cell in the cuvette sample cell holder 4 Press Options More Standard Addition An overview of the data of the standard addition procedure is displayed 5 Press OK to accept the standard values for standard concentration sample volume total and standard addition volume Press Edit to change any of th...

Page 85: ...ows the reading of the samples with and without added standard The third column shows the detection rate of the added standard Note The black highlighted line is active 8 The reading of the sample in the instrument without added standard is automatically shown under 0 mL 9 Prepare the standard addition solution as described in the procedure 10 Use the arrow keys to select the first standard additi...

Page 86: ...a points are to the line If the correlation coefficient 1 the curve is linear The concentration shown above the curve is the estimated concentration of the sample without the added standard Note In the curve menu the name on the Curve key switches to Table Press Table to display all the data in the table again 12 Press Ideal line to display the relationship between the added standard solutions and...

Page 87: ...ns for Parameter Setup Table 12 Single wavelength setup options Options Description More For further Options Store Off On With the Store On setting all measurement data are stored automatically With the Store Off setting no measurement data are stored Trans Abs To switch to transmittance concentration or absorbance readings λ Wavelength To enter the measurement wavelength Use the alphanumeric keyp...

Page 88: ...tion and press OK to confirm Reading Mode Single Reading Mode A reading is only displayed after a measurement has been carried out press Read standard setting see section 6 4 2 on page 89 Continuous Reading Mode After the zero measurement all readings are displayed automatically and continuously see section 6 4 3 on page 89 Save as User Program To store the selected parameters as a User Program se...

Page 89: ...2 Insert the sample cuvette cell into the cuvette sample cell holder Press Read 3 For data storage see section 5 3 2 on page 38 6 4 3 Take single wavelength measurements continuous readings 1 Insert the blank cuvette cell into the cuvette sample cell holder Press Zero Note In the reading mode Continuous only the Zero key is shown to start the reading The reading sequence is started automatically 2...

Page 90: ...ulti wavelength setup options Options Description More For further Options Store Off On With the Store On setting all measurement data are stored automatically With the Store Off setting no measurement data are stored Trans Abs To switch to transmittance concentration or absorbance readings λ Wavelength To enter the measurement wavelength Use the alphanumeric keypad to enter the measurement wavele...

Page 91: ...K 1 A 1 K 2 A 2 K 3 A 3 K 1 A 1 K 2 A 2 K 3 A 3 K 4 A 4 K 1 A 1 K 2 A 2 K 1 A 1 K 2 A 2 K 3 A 3 K 1 A 1 K 2 A 2 K 3 A 3 K 4 A 4 A 1 refers to the absorbance at wavelength 1 A 2 refers to the absorbance at wavelength 2 etc K 1 refers to the coefficient at wavelength 1 K 2 refers to the coefficient at wavelength 2 etc Coefficients can be set negative where subtraction is required Recall Data Call up...

Page 92: ...5 significant digits with a maximum of 4 significant digits after the decimal point Concentration factor 1 Press Concentration Factor Off in the Options menu Press On to highlight this feature 2 Press the Factor key to enter the factor by which absorbance readings are to be multiplied Press the Unit key to select the units for concentration measurements or to create a new unit 3 Press OK to confir...

Page 93: ...e cell into the cuvette sample cell holder Press Read Note The UV lamp is not switched on until a wavelength in the UV spectrum has been entered and the blank reading has been started During the warm up phase of the UV lamp the message Warming up is displayed and the UV lamp symbol flashes As soon as the UV lamp is ready the blank reading is carried out Note Zero and Read are disabled until the ce...

Page 94: ...or Parameter Setup Table 14 Wavelength scan setup options Option Description More For further Options Store icon To store the scan data Reference Off On From the displayed list of stored scans a record is selected for use as a reference scan superimposed scan This can be highlighted or shown in the background in comparison with the actual measured scan Note This option is only available when there...

Page 95: ...ings of high resolution data take a longer time than recordings of low resolution data Selecting a larger step allows the instrument to scan faster but decreases the resolution of the collected data Scale Units Scale In the automatic scaling mode the y axis is automatically adjusted so that the total scan is displayed The manual scaling mode allows sections of the scan to be displayed Units Choice...

Page 96: ...ing table 1 Press Select View in the Options menu after a reading is taken 2 A table with the results is displayed 3 To return to the graph press Options and then View Graph Cursor mode 1 Press Cursor Mode Track in the Options menu 2 The selection for this menu item determines what data are displayed in the table Highlight Track or Peak Valley 3 Press OK to confirm 4 Press Return to return to the ...

Page 97: ... ymax The graph is adjusted to display only the values in the selected range If automatic scaling is selected the instrument sets the limits automatically so that the total range can be displayed 4 Press OK 5 Press Return to return to the scan mode 6 6 2 Wavelength scan reading After the scanning parameters have been selected the baseline must be scanned Changing any of the scanning parameters req...

Page 98: ...avelength scan analysis Table 15 Navigating the wavelength scan Cursor Function Zoom Function Description Curve Icon Choice of Cursor Mode Choice of Cursor Mode Peak Valley cursor moves between minimum maximum absorbance values or Cursor Mode Tracking cursor moves over each data point of the scan Arrow keys The arrow keys are used right left to move the cursor depending on the selected mode to the...

Page 99: ... be overlaid 2 The reference curve is shown in gray The absorbance or transmittance value and the associated wavelength are highlighted in gray Note A black and an gray box are shown in the left corner of the display The gray box relates to the reference scan and the black one relates to the current wavelength scan 3 To complete the wavelength scan reading see section 6 6 2 on page 97 The newly pl...

Page 100: ... On Note Only scans that have the same wavelength range and step can be displayed using the overlay option A reference curve can only be displayed with another active curve Two previously stored scans cannot be overlaid 4 The reference curve is shown in gray The absorbance or transmittance value and the associated wavelength are highlighted in gray Additionally the difference of the extinction and...

Page 101: ... time for data collection and the time interval between the collection of data points λ To input the wavelength setting View Table Graph To display readings in absorbance transmittance or concentration This can be changed after sample data are collected Scale Units Scale In the automatic scaling mode the y axis is automatically adjusted so that the total scan is displayed The manual scaling mode a...

Page 102: ... the alphanumeric keypad to set the limits ymin and ymax The graph is adjusted to display only the values in the selected range If automatic scaling is selected the instrument sets the limits automatically so that the total range can be displayed 4 Press OK to confirm 5 Press Return to return to the scan mode 6 7 2 Time course scan reading After the parameters have been selected the instrument mus...

Page 103: ... also shown in the table Select Stop to stop taking sample readings 6 7 3 Analysis of time course data After the data are collected the following manipulations can be done on the graphic data The Time Course Program is complete if the sound is turned on the instrument beeps when the readings are done the graph is shown fullsize the scaling of the x axis occurs automatically the Cursor functions in...

Page 104: ...The difference to the fixed cursor is shown on the curve The delta values are correspondingly highlighted and displayed on the x and y axes The gradient of the curve and the correlation coefficient r2 between the cursor points in the Delta mode are shown under the curve Cursor Mode Single The cursor moves to each selected measurement point of the scan Arrow keys The arrow keys right left are used ...

Page 105: ...m Checks in the Main Menu The System Checks menu contains instrument information and various performance tests 6 8 1 Instrument information 1 Press Instrument Information in the System Checks menu 2 The model serial number and software version are displayed ...

Page 106: ...automatically and the software is updated 6 Press OK to return to the System Checks menu Note When the instrument software has been updated a prompt to restart the instrument is displayed 6 8 3 Optical checks For each optical check the measured results must be evaluated against user specific requirements The check options do not define tolerances 1 Press Optical Checks in the System Checks menu Th...

Page 107: ...elength accuracy at 656 1 and 486 0 nm and to check bandwidth at 656 1 nm only 1 Press Wavelength Check in the Optical Checks menu 2 Press λ to select the wavelength 3 Make a choice and confirm with OK 4 Press Start Note During the warm up phase of the UV lamp the message Lamp Warmup is displayed ...

Page 108: ...e Check test is used to test the photometric noise in the instrument This test can be used to test noise at any wavelength and at an absorbance level determined by a specific sample 1 Press Noise Check in the Optical Checks menu 2 Press λ to input the wavelength 3 Input the wavelength and press OK to confirm 4 Press Zero ...

Page 109: ...esult will be displayed Thirty readings are averaged for the blank The Average and Std Deviation are calculated from 100 consecutive absorbance readings Noise Sample Reference Note Failings and passings will be defined by the user 8 Press PC Printer icon to send data to a PC or Printer 9 Press Cancel to return to Optical Checks ...

Page 110: ...ight in the instrument at 220 0 nm and 340 0 nm 1 Press Stray Light Check in the Optical Checks menu 2 Press λ to select the wavelength 3 Make a choice and confirm with OK 4 Press Zero 5 Insert the sample cuvette cell or reference filter into the cell compartment Close the cell compartment Press Start ...

Page 111: ... test the photometric accuracy and repeatability of the instrument This test can be used to test absorbance at any wavelength by a specific sample or test filter set Refer to section 6 8 3 6 on page 113 1 Press Absorbance Check in the Optical Checks menu 2 Press λ to input the wavelength 3 Input the wavelength and press OK to confirm 4 Remove any cuvette sample cell from the cell compartment close...

Page 112: ...results must be evaluated against user defined requirements 8 Press Cancel to return to Optical Checks 6 8 3 5 Drift check The Drift Check test is used to test the stability of the instrument Note The Drift Check runs 1 hour 1 Press Drift Check in the Optical Checks menu 2 Press λ to input the wavelength 3 Input the wavelength and press OK to confirm 4 Press Start ...

Page 113: ...r the overall values are calculated and displayed The test proceeds to completion 7 Press Cancel to return to Optical Checks 6 8 3 6 Verification kit The Verification Kit LZV 537 see Section 9 on page 129 is designed for periodic monitoring of scattered light photometric accuracy and the wavelength accuracy of the spectrophotometers When results exceed allowable tolerances given in the quality con...

Page 114: ...t Photometrical accuracy Wavelength accuracy 4 Press OK when all values are entered and the overview is displayed 5 Press Verification 6 Insert the Multi Cell Holder in the cell compartment in such a way that the cell opening for 10 mm square cuvettes cells is at the front Secure the holder with two locking screws 7 Remove any cuvettes cells from the cell compartment close the cell compartment and...

Page 115: ...cation csv 6 8 4 Output checks If a printer is connected a test printing of the current screen will be printed 6 8 5 Lamp history The Lamp History menu provides the following information The amount of time that the lamp has been on Hours The number of times that the lamp has been turned on Cycles The number of times that the instrument tried to turn the UV lamp on triggers After a lamp is replaced...

Page 116: ...ry reference for the service times can be entered After switching the instrument on this memory reference will be activated and indicated at the appropriate time 1 Press Service Time in the System Checks menu 2 Select On and then Last Service to enter the date of the last inspection 3 Press OK to confirm 4 Select Next Service to determine a specific period of time up to the next inspection 5 Press...

Page 117: ...next service date the Instrument Backup menu offers the possibility to store all programs measuring data Operator ID Sample ID passwords and all adjustable data on a USB stick 1 Press Instrument Backup in the System Checks menu 2 Connect the USB memory stick section 3 3 on page 14 3 Press Store to start a Backup Note If the USB stick is not connected the message Please insert USB Memory is display...

Page 118: ... message Instrument Backup is stored to USB stick will be displayed 4 Press OK to return to the System Checks menu Restore backup data Important Note All current data will be overwritten when restoring the Backup file 1 Press Instrument Backup in the System Checks menu 2 Connect the USB memory stick containing the Backup section 3 3 on page 14 3 Press Restore to pass back the data ...

Page 119: ...ge Please insert USB Memory is displayed Connect a USB stick in order to store the data Press OK to confirm and press Restore again 4 Press OK to confirm after the message Instrument Backup from S N XXXXXXX Restore is displayed 5 After the backup start the instrument again ...

Page 120: ...120 Advanced Operations ...

Page 121: ...ert a brush or sharp object into Cell Compartment 1 to avoid damaging the mechanical components Dry the cleaned parts carefully with a soft cotton cloth 7 1 2 Display Take care not to scratch the display Do not touch the screen with ball pens pencils or similar pointed objects Clean the display with a soft lint free and oil free cotton cloth Diluted window cleaner liquid can also be used 7 1 3 Cuv...

Page 122: ...witched off until the change of lamp has been completed WARNING Burn Hazard Wait until the lamp cools down Contact with the hot lamp can cause burns 3 Use a screwdriver to remove the cover from the back of the instrument the screws may be slotted or cross headed 4 Place the cover and the attached fan carefully beside the instrument take special care with the fan cable 5 Push the spring Figure 8 on...

Page 123: ... page 124 item 4 from the socket by pushing down on the safety contact 6 Use a screwdriver to unscrew the two fastening screws Figure 8 item 3 the screws may be slotted or cross headed out of the socket 7 Holding the lamp socket lift the deuterium lamp up out of the lamp compartment remove the complete unit including the cable 8 Carefully insert the new deuterium lamp into the lamp compartment Imp...

Page 124: ...ication of a problem with the equipment If fuse failure continues contact a service representative for instructions on how to return the equipment for repair Do not attempt to repair the equipment yourself 1 Switch the instrument off 2 Unplug the power cord to remove all power from the instrument Figure 8 Lamp compartment 1 Tungsten lamp 5 Plug contacts for fan 2 Spring 6 Plug contact for deuteriu...

Page 125: ...nd cuvette sample cell adapter from the cell compartment 2 Turn the instrument off 3 Unplug the power cord 4 Lift the instrument and check the color of the filter mat Replace the filter mat if is dark gray or black 7 4 1 Filter pad replacement 5 Carefully turn the instrument over and place it on a soft surface 6 Use a screwdriver standard or cross head to open the filter grid Figure 9 on page 126 ...

Page 126: ...126 Maintenance Figure 9 Base of DR 5000 with filter grid 1 Filter grid 3 Phillips screw 2 Filter pad Figure 10 Change of filter pad 1 Filter pad ...

Page 127: ...he instrument Check the lamp and replace if necessary Close the lid Press Start Again Error Selfcheck stopped Please remove the cuvette Please close the lid Self Check Test stops while starting the instrument Remove the cuvette sample cell from the cell compartment Press OK Error Sipper Kink in tube Peristaltic pump failure Check the tube connections Error Selfcheck stopped Hardware error Error x ...

Page 128: ...128 Troubleshooting ...

Page 129: ...cation Kit 6 precision glass filters with target values LZV537 Semi Micro Cell 50 mm Glass LZP269 Macro Cell 20 mm Glass LZP331 Sample Cell 1 cm Square Glass 10 mm path length matched pair 2095100 Sample Cell 1 cm Square Quartz 10 mm path length matched pair 4822800 Sample Cell 50 mm Rectangular Glass 2629250 Sample Cell 50 mm Rectangular Quartz 2624450 Sample Cell 100 mm Rectangular Glass 2629201...

Page 130: ...130 Replacement Parts ...

Page 131: ...hach lange nl www hach lange nl HACH LANGE APS Åkandevej 21 DK 2700 Brønshøj Tel 45 36 77 29 11 Fax 45 36 77 49 11 info hach lange dk www hach lange dk HACH LANGE AB Vinthundsvägen 159A SE 128 62 Sköndal Tel 46 0 8 7 98 05 00 Fax 46 0 8 7 98 05 30 info hach lange se www hach lange se HACH LANGE S R L Via Riccione 14 I 20156 Milano Tel 39 02 39 23 14 1 Fax 39 02 39 23 14 39 info hach lange it www h...

Page 132: ...132 Contact Information ...

Page 133: ...caused by any repair or attempted repair not authorized by Hach Company Any product not used in accordance with the instructions furnished by Hach Company Freight charges to return merchandise to Hach Company Freight charges on expedited or express shipment of warranted parts or product Travel fees associated with on site warranty repair This warranty contains the sole express warranty made by Hac...

Page 134: ...134 Limited Warranty ...

Page 135: ... Integral 94 96 Interfaces 29 Internet 58 106 L Language 19 M Main Menu 22 Maintenance 121 Mark 103 Measurement Process 54 55 72 Measuring sequence 73 Multi Wavelength 22 64 66 90 91 92 93 N Name 54 Noise Check 108 O Operating environment 13 Operator ID 23 creating 23 delete 23 Optical Checks 106 107 108 110 111 112 113 Output Checks 115 P Password 32 activate 32 PC and Printer 29 31 Print data 31...

Page 136: ...6 87 90 Touch Screen 21 U Unpack the instrument 13 Update 54 58 106 User Programs 22 61 V Variables 54 56 78 Verification Kit 113 Version 54 55 View Table 101 W Warranty 133 Wavelength 87 90 Wavelength Check 107 Wavelength Range 95 Wavelength Scan 22 38 39 40 41 94 95 98 Wavelength setting 101 Wavelength spectrum 94 ...

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