GE Ettan IPGphor 3, Instrument User Manual

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Ettan IPGphor 3 Isoelectric Focusing System function and description
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Ettan IPGphor 3 Instrument User Manual 11-0034-58 Edition AB
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1 Ettan IPGphor 3 Isoelectric Focusing System function
and description
The Ettan™ IPGphor™ 3 Isoelectric Focusing System performs the first dimension
isoelectric focusing (IEF) step in 2-dimensional (2-D) electrophoresis. The system
comprises the Ettan IPGphor 3 unit, combined with separate strip holders or a
manifold. Two types of manifolds are available; the ceramic manifold and the
light manifold. The IEF separation protocols are programmed, run and controlled
from the Ettan IPGphor 3 control panel or from a PC running the Ettan IPGphor
Control Software.
The Ettan IPGphor 3 Isoelectric Focusing System includes the following features:
• an integrated high-voltage DC power supply delivering up to 10 kVolts
• temperature control by Peltier elements
• light exclusion during the run. Compatible with CyDye™ labeled proteins
and other light sensitive stains
• IEF runs are programmed from the Ettan IPGphor 3 control panel with up to
ten user-defined IEF protocols, each with up to 9 steps per protocol
• programmable functions including: rehydration time, platform temperature,
current limit, voltage limit for each step, voltage gradient or step and step
duration
• Ettan IPGphor 3 Control Software. From a PC, protocols are created,
graphically displayed and stored on the Ettan IPGphor 3 unit. The software
records the run parameters over time and presents data as graphs and log
files. Data is saved or can be exported to Microsoft® Excel
• Ettan IPGphor 3 Control Software allows easy programming and
recommended protocols are generated by providing instrument
configuration, IPG strip length and pH gradient
• strip holders for rehydration and automated running
• Ettan IPGphor Cup Loading Manifold for easy running of up to 12 IPG strips.
Cup loading improves focusing results particularly when using basic IPG
strips
IMPORTANT! The Ettan IPGphor 3 system is intended for research use only
and should not be used in any clinical or in Vitro procedures for diagnostic
purposes.